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28 December 2019, Volume 35 Issue 12 Previous Issue    Next Issue

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Co-inhibitory Immune Checkpoints in Oral Squamous Cell Carcinoma SUN Zhijun, WU Lei 2019, 35(12): 1107-1110.  DOI: 10.13701/j.cnki.kqyxyj.2019.12.001 Abstract ( 348 )   PDF (812KB) ( 509 )   Clinical breakthroughs have been made in the treatment of cancer by immune checkpoint, especially by the antibody of PD-1.However, there are still many cancer patients who are not responsive to immune checkpoint blockade. Therefore, it is necessary to further study the mechanism of immune checkpoint, which could be exploited for oral squamous cell carcinoma (OSCC). In this review, we summarize the expression and the mechanism of partial co-inhibitory immune checkpoint molecules and discuss the possibility of targeting co-inhibitory immune checkpoints for the treatment of OSCC. References | Related Articles | Metrics

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Research Progress on Mechanism of Cleft Palate Caused by MicroRNA GUO Jianan, HE Wei 2019, 35(12): 1111-1114.  DOI: 10.13701/j.cnki.kqyxyj.2019.12.002 Abstract ( 254 )   PDF (752KB) ( 398 )   MicroRNAs are a class of small non-coding RNAs that participate in the development of embryos by regulating the expression of their target genes. For a long time, scholars have focused on protein-coding mRNA. However, in recent years, with the in-depth study of non-coding RNAs, especially miRNAs, the mechanism of their role in palatal development is gradually elucidated. Therefore, this review summarizes the recent progress on the mechanism of cleft palate caused by miRNAs, hoping to provide a new reference and perspective for the cleft lip and palate researchers. References | Related Articles | Metrics

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Root Resorption of Clear Aligner Treatment XIA Lulu, HUA Xianming 2019, 35(12): 1115-1118.  DOI: 10.13701/j.cnki.kqyxyj.2019.12.003 Abstract ( 313 )   PDF (959KB) ( 384 )   Clear aligner is popular to patients for the advantages of invisibility, comfortability, aesthetics, hygiene, and predictability. Root resorption is an issue that orthodontists cannot avoid in clinical practice. Compared with conventional fixed appliances, aligners have obvious technical advantages, but their root resorption problems cannot be avoided. Clear aligner is a private custom-made removable appliance. Therefore, the root resorption in clear aligner treatment is not the same as that in the conventional fixed appliance treatment. The material and mechanical properties are different among aligners of different company. This article intends to review the researches about the root resorption in clear aligner treatment and the factors' analyses so as to provide a theoretical basis for the clinical application of clear aligners. References | Related Articles | Metrics

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Research Progress on Sclerostin Antibody LIANG Tingting, GUO Lvhua 2019, 35(12): 1119-1121.  DOI: 10.13701/j.cnki.kqyxyj.2019.12.004 Abstract ( 501 )   PDF (735KB) ( 355 )   Sclerostin is a negative regulator of bone formation and inhibits bone formation by inhibiting the Wnt/β-catenin signaling pathway. Sclerostin antibody (Scl-Ab) ensures the transmission of the Wnt/β-catenin signaling pathway by antagonizing the negative regulation of sclerostin on bone formation. Compared to other osteogenic drugs, Scl-Ab can stimulate osteogenic activity without stimulating osteoclast activity. A variety of Scl-Ab have been developed. Romosozumab, the most representative antibody, has completed clinical phase III trials, demonstrating that it can significantly promote bone formation and inhibit bone resorption. However, clinical efficacy and safety of Scl-Ab is still unclear and requires further investigation and discussion. Therefore, this review was conducted in order to provide theoretical basis for experimental research and clinical treatment. References | Related Articles | Metrics

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Research Progress of Matrix Metalloproteinases in Caries LI Jingya, HUANG Yang 2019, 35(12): 1122-1124.  DOI: 10.13701/j.cnki.kqyxyj.2019.12.005 Abstract ( 290 )   PDF (737KB) ( 307 )   The proteolylic enzymes need Zn2+ and Ca2+ as cofactors, hence the name of matrix metalloproteinases(MMPs). Their family members can degrade most of the extracellular matrix. Type I collagen in dentin is also the substrate of MMPs. Most studies have shown that matrix metalloproteinases not only participate in maxillofacial neoplasms and mucosal diseases, but also participate in the pathological process of the soft and hard tissues of teeth, and periapical and periodontal lesions. MMPs have an vital function on the pathological changes of dental hard tissues and the process of restoration and treatment. MMPs can be detected, no matter whether it is sound or decayed dentin. This review has discussed the effect of MMPs in caries. References | Related Articles | Metrics

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Establishment of Hypoxic Oral Cancer Model by Elevating Pedicled Flap on Dorsal Skin in Nude Mouse SONG Yong, MA Tingting, WANG Junlin, LIU Yanjie, CHEN Yucong, HE Hujie 2019, 35(12): 1125-1128.  DOI: 10.13701/j.cnki.kqyxyj.2019.12.006 Abstract ( 194 )   PDF (5337KB) ( 258 )   Objective: To construct a hypoxic oral cancer model in nude mouse and provide a basis for tumor hypoxic-related studies in vivo. Methods: The pedicled skin flaps supplied by unilateral deep iliac circumflex artery were designed on the back of nude mice. After the flaps were elevated and sutured in situ, the optimal site of hypoxia was determined by morphological observation and HIF-1alpha detection. After tumor formation by inoculation of oral cancer cells subcutaneously in nude mice, flaps with tumors were incised and valved in the same way. Immunohistochemical staining was used to detect the expression of HIF-1alpha in oral cancer cells of nude mice. Results: When the length-width ratio of pedicled dorsal skin flaps in nude mice was 2-3:1, the color of the flaps was normal, the structure and morphology of skin squamous cells in distal 1/3 parts kept unchanged in which the expression of HIF-1alpha was significantly up-regulated. Furthermore, the expression of HIF-1alpha in tumor cells was significantly increased 48 hours after tumorigenesis by flapping, which confirmed the dynamic changes of hypoxic state in tumors. Conclusion: The present study provided a simple and feasible way to establish hypoxic model of oral cancer supplied by unilateral deep circumflex iliac artery in nude mice which can be applied as a good animal model for hypoxic-related research in vivo. References | Related Articles | Metrics

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Expression and Function of Splicing Factor hnRNP F in Head and Neck Squamous Cell Carcinoma XIONG Yingjie, JIA Rong 2019, 35(12): 1129-1131.  DOI: 10.13701/j.cnki.kqyxyj.2019.12.007 Abstract ( 187 )   PDF (983KB) ( 244 )   Objective: To investigate the expression and function of hnRNP F in head and neck squamous cell carcinoma (HNSCC). Methods: The expression of hnRNP F in head and neck squamous cell carcinoma was analyzed in TCGA and Oncomine database. The relationship between hnRNP F expression and disease-free survival in HNSCC was analyzed in cbioportal database. CAL-27 cells were transfected with anti-hnRNP F siRNA or non-specific siRNA. The growth curve of cells was analyzed by cell counting. Results: HnRNP F was overexpressed in HNSCC patients. Patients with high expression of hnRNP F showed significantly poorer disease-free survival than those with low expression of hnRNP F. Knockdown of hnRNP F significantly inhibited CAL-27 cell growth. Conclusion: hnRNP F may play important roles in the development of HNSCC. References | Related Articles | Metrics

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Prognostic Factors of Primary Intraosseous Squamous Cell Carcinoma of the Jaw GUO Lingyan, CHEN Fen, WU Pingfan, LI Yu, LEI Zhengge, CHEN Linlin 2019, 35(12): 1132-1136.  DOI: 10.13701/j.cnki.kqyxyj.2019.12.008 Abstract ( 182 )   PDF (743KB) ( 373 )   Objective: To determine the prognostic factors of primary intraosseous squamous cell carcinoma(PIOSCC)of the jaw. Methods: Patients with PIOSCC of the jaw admitted to the Department of Oral and Maxillofacial Surgery,Affiliated Stomatological Hospital of Nanchang University (2012-2018) were selected as the study subjects. The clinical, radiological, and histopathological data of these patients were collected and followed up. Descriptive statistical analysis was used to determine the prognostic factors of the disease. Results: A total of 21 PIOSCC patients were included in this study. The mean age at diagnosis was 53.7 years (ranged from 41 to 77 years). Of the 21 patients, there were 13 males and 8 females, and 8 cases in maxilla and 13 cases in mandible. During the follow-up period, 2 cases were died of local recurrence and 5 cases were died of distant metastasis. The overall survival rate was 66.7%. Tumor size, cervical lymph node metastasis, and clinical stage of TNM were the negative prognostic factors. Conclusion: The prognosis of patients is poor if the tumor is more than 4 cm in size or with cervical lymph node metastasis. References | Related Articles | Metrics

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Correlation between Invasive Depth of Gingival Squamous Cell Carcinoma and Lymph Node Metastasis YU Ying, QU Weiguo, SU Yingying, ZHANG Qian, FENG Kai 2019, 35(12): 1137-1140.  DOI: 10.13701/j.cnki.kqyxyj.2019.12.009 Abstract ( 226 )   PDF (4475KB) ( 403 )   Objective: To analyze the correlation between infiltration depth and other related influencing factors and lymph node metastasis, and to determine whether the depth of invasion can be used as a predictor of lymph node metastasis. Methods: Thirty patients with gingival squamous cell carcinoma were enrolled in the study. 30 patients with preoperative lymph node negative (cN0) were enrolled in the study. The clinical factors (age, gender, T classification) and pathological factors (invasion depth, degree of differentiation, invasion mode, and peritumoral lymphocyte density) of gingival squamous cell carcinoma were reviewed. Results: Univariate logistic regression analysis showed that the depth of invasion and tumor invasion were correlated with lymph node metastasis. There was no significant correlation between gender, age, pathological grade, cTNM stage, and peritumoral lymphocyte infiltration density and lymph node metastasis. In multivariate logistic regression analysis, only the depth of invasion was an independent predictor of cervical lymph node metastasis. Conclusion: Invasion depth is an important factor in the regional lymph node metastasis of gingival squamous cell carcinoma. It is important for the evaluation of regional lymph node metastasis and can be used as a predictor of lymph node metastasis. References | Related Articles | Metrics

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Protective Effects of Hyaluronic Acid on Synovial Fibroblasts from Temporomandibular Joint Against Oxidative Stress Induced by Hydrogen Peroxide CAI Hengxing, LI Yingjie, LONG Xing 2019, 35(12): 1141-1144.  DOI: 10.13701/j.cnki.kqyxyj.2019.12.010 Abstract ( 163 )   PDF (2196KB) ( 223 )   Objective: To observe the effects of oxygen free radicals on the synovial fibroblasts from the temporomandibular joint and the protective effects of hyaluronic acid against the oxidative stress. Methods: Synovial tissues were harvested from the rabbit temporomandibular joint and cultured en bloc. The synovial fibroblasts gained at 3-8 passages were used for further study. Hydrogen peroxide was introduced to mimic the effect of oxygen free radicals within the temporomandibular joint, and the hyaluronic acid was added to counteract the effects of reactive oxygen species. Results: There was no difference in the vitality of synovial fibroblasts between H2O2 groups (5 μmol/L and 10 μmol/L) and control group (P＞0.05),while the 100 μmol/L H2O2 overinhibited the viability of the synovial fibroblasts. 50μmol/L H2O2 appropriately inhibited the activity of synovial fibroblasts (60%) and was selected as the ideal stimulus. In the serial concentrations of hyaluronic acid, the 0.01% and 0.05% groups significantly recovered the activity of synovial fibroblasts in contrast to the low or high concentrations of HA (P＜0.05). Conclusion: Proper oxidative stress to the synovial fibroblasts can be induced by 50 μmol/L H2O2, while the 0.01% and 0.05% HA can protect the synovial fibroblasts from the damage of oxygen free radicals. References | Related Articles | Metrics

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CBCT Imaging of Temporomandibular Joint in Patients with Unilateral Crossbite and Temporomandibular Joint Disc Displacement GU Wenyan, ZHOU Yuchuan, CHEN Qingli, JIA Mengying, GONG Zhongcheng 2019, 35(12): 1145-1148.  DOI: 10.13701/j.cnki.kqyxyj.2019.12.011 Abstract ( 222 )   PDF (2689KB) ( 173 )   Objective: To study the CBCT imaging features of temporomandibular joint in patients with unilateral crossbite and temporomandibular joint disc displacement (DD). Methods: A total of 52 patients with unilateral crossbite and DD were enrolled in the clinical data and CBCT imagings were performed. The condyle-joint socket was reconstructed using Mimics 22.0 software, and the joint depth, joint nodule slope, joint space were measured. Data were analyzed using SPSS22.0 software. Results: The anterior space was increased in the patients with unilateral crossbite and DD. According to the position of the crossbite in the oral cavity (whether the anterior and posterior teeth were crossbite), the anterior and upper spaces in patients whose anterior and posterior teeth were non-crossbite were bigger than those had crossbite (P<0.05). Conclusion: Crossbite can cause imaging changes in the condyle-joint socket, resulting in widening of the anterior space of the affected side. CBCT can better show the changes of the skeletal structure and joint space of the temporomandibular joint. References | Related Articles | Metrics

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Curative Effect of Three Different Therapy Methods for Temporomandibular Joint Synovitis LV Yaoguang, SONG Zhiqiang, JIA Mengying, SHAO Bo, ZENG Xuemin, GONG Zhongcheng 2019, 35(12): 1149-1152.  DOI: 10.13701/j.cnki.kqyxyj.2019.12.012 Abstract ( 237 )   PDF (732KB) ( 277 )   Objective: To compare the efficacy difference of three methods in the treatment of temporomandibular joint synovitis. Methods: 56 cases of the patients who were diagnosed as TMJ synovitis were collected and divided into 3 groups They were treated as: the injection of sodium hyaluronate on articular upper cavity, injection combining with local smearing of diclofenac diethylamine emulgel, and the injection combining with stabilization splint. Two weeks later, the differences of VAS score and temporomandibular joint dysfunction index (Fricton index) between three methods were compared. Results: Compared with the only injection group, the VAS grade of the groups combined with diclofenac diethylamine emulgel smearing and combined with stabilization splint decreased significantly (P<0.05), and the group combined with splint was more effective (P<0.05). As to the Fricton index, there was also a difference for the DI value and CMI value among three group (P<0.05). However, the PI in the group combined with stabilization splint decreased significantly (P<0.05). Conclusion: All three methods have therapeutical effect on the temporomandibular joint synovitis, while the injection combined with stabilization splint is more effective on the temporomandibular joint synovitis, and has better relaxation effect on muscular tenderness. References | Related Articles | Metrics

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Application of 3D Printed Cyst Plug in Mandibular Cystic Lesions LIANG Yan, MA Wen, NIE Yan, FU Shuai, ZHANG Changbin, CUI Qingying, WANG Lidong, LI Ming 2019, 35(12): 1153-1156.  DOI: 10.13701/j.cnki.kqyxyj.2019.12.013 Abstract ( 268 )   PDF (2062KB) ( 245 )   Objective: To investigate the application of 3D printed cyst plug in the mandibular cystic lesion. Methods: Forty patients with mandibular cystic lesion were randomly divided into the control group and 3D printed cyst plug group. The control group was made with traditional cyst plug, and 3D printed cyst plug group was digitally designed surgical plan and 3D printed cyst plug through a preoperative model and oral examination. Two groups of patients were followed up for a long time. The reduction rate of the cyst volume was compared, and the patients' satisfaction with the cyst plug was studied. Results: The volume of the cyst was reduced in both groups, and the reduction of cyst volume in the first 3 months after surgery was more obvious than that in the next 3 months. The group of 3D printed cyst plug was better than the control group in the comfort (P＜0.05) and wearing (P＜0.05), but there were no significant differences in the retention between two groups (P＞0.05). Conclusion: The 3D printed cyst plug is an effective device to keep the pressure balance of cyst after decompression. References | Related Articles | Metrics

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A New Type of 3D Printed Orthodontic Microimplant Template TANG Rui, SUN Jianwei, LI Chenghua, LIU Xin 2019, 35(12): 1157-1161.  DOI: 10.13701/j.cnki.kqyxyj.2019.12.014 Abstract ( 245 )   PDF (3239KB) ( 329 )   Objective: To develop a 3D printed orthodontic microimplant template which could be applied in clinical orthodontic implantation of microimplant anchorage and improve the implantation accuracy. Methods: The patients needed to be implanted microimplant anchorage were sent for CBCT and intraoral scan. The CT data stored in DICOM format and STL data scanned with intraoral scan were imported into 3Shape Implant Studio for fusion, so as to reconstruct the 3D model of maxillary and mandible bone. On the maxillary and mandible bone model reconstructed, the implant guide plate was designed according to the requirements of vertical bone surface of the microimplant, i.e. no damage to the root, maxillary sinus and other tissues, and as far as possible parallel to the root of the tooth, and the guide plate was printed with Formlabs2 3D printer. During the operation, the position and angle of the implant were determined by the guide plate, and the microimplant was implanted through the guide tube of the plate. After operation, the patients should take CBCT scanning that was used to evaluate the position and angle of the implant. Results: It was proved by CBCT that when the new type of orthodontic microimplant template was used during the operation, the position and angle of the microimplant were accurate and the microimplant did not loosen and fall out 3 months after the operation. Conclusion: 3D printed orthodontic microimplant guide plate was developed to improve the accuracy of implant placement and angle, prevent tooth root damage, and improve implant stability. References | Related Articles | Metrics

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Changes of Lip Morphology from Frontal View in Orthodontic Patients with Bimaxillary Protrusion PAN Yue, YANG Andi, LEI Lang 2019, 35(12): 1162-1165.  DOI: 10.13701/j.cnki.kqyxyj.2019.12.015 Abstract ( 250 )   PDF (1210KB) ( 461 )   Objective: To analyze the changes in the frontal morphology of lips during orthodontic treatment. Methods: Thirty-nine female patients (23.1±4.1) years who were diagnosed with bimaxillary protrusion were enrolled in the bimaxillary protrusion group. After the removal of the four first premolars, orthodontic treatment was performed with straight wire arch techniques. The control group consisted of 19 female volunteers (26.0 ± 2.6) years with class I individual normal occlusion. Facial photographs, intraoral images, and lateral cephalograms were taken before and after active orthodontic treatment in the resting position. Image pro-Plus 6.0 software was used to measure the vermilion height and lip area in the frontal photographs. Then incisors retraction and inclination were measured and the correlation of between incisors retraction and lip morphology were explored. Results: The vermilion height and lip area before orthodontic treatment in the bimaxillary protrusion group were significantly greater than the control group, and vermilion height as well as lip area was significantly reduced after orthodontic treatment. No difference in the upper lip area and vermilion height was observed between the patients with bimaxillary protrusions after treatment and the control group, whereas the area of the lower lip in bimaxillary patients after treatment was significantly larger than that of the control group. Orthodontic treatment significantly reduced the tooth inclination of patients with bimaxillary protrusion, and the incisor retraction was weakly correlated with the reduction of the vermilion height in the lateral medium. Conclusion: Lip morphology from the front view was significantly improved in bimaxillary protrusive patients by orthodontic treatment. References | Related Articles | Metrics

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Development and Application of a New Type of Soft Tissue Measuring Ruler Assisted in Obtaining Natural Head Position GUAN Huijuan, FENG Cuijuan, WANG Qingqing, CAO Zhengfei, PANG Zimeng, HU Qingbo 2019, 35(12): 1166-1168.  DOI: 10.13701/j.cnki.kqyxyj.2019.12.016 Abstract ( 173 )   PDF (1140KB) ( 335 )   Objective: To develop a new type of soft tissue measuring ruler to facilitate obtaining the natural head position. Methods: Through repeated research and experiment, a soft tissue measuring ruler was designed to assist in obtaining natural head position. It was compared with traditional computer measurement methods to observe its accuracy. 30 clinical patients were randomly selected, and some soft tissue indexes were measured using the measuring ruler. Soft tissue measurements were also taken on the patient's lateral phase using Winceph 9.0 software. Differences between the two measurement methods were compared. Results: There was no significant difference between two measurement methods (P＞0.05). However, when measuring the same data, the measuring time of the new ruler was much smaller than that with computer (P＜0.05). Conclusion: The soft tissue measuring ruler which assists obtaining natural head position can accurately measure various soft tissue indexes and incisor position, and it is more time-saving and convenient for clinicians. References | Related Articles | Metrics

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Effects of Fas Ligand Overexpression on Osteogenic Differentiation Potential of Human PDLSCs MA Lingzhi, REN Yalan, QIAN Liping, GE Wenbin, LIU Yali, ZHOU Zhi 2019, 35(12): 1169-1172.  DOI: 10.13701/j.cnki.kqyxyj.2019.12.017 Abstract ( 212 )   PDF (2326KB) ( 372 )   Objective: To investigate the effect of FasL overexpression on osteogenic differentiation of human periodontal ligament stem cells (PDLSCs). Methods: Human PDLSCs were cloned and cultured in vitro with limited dilution. FasL overexpression plasmid was constructed and transfected into PDLSCs. The experiment was divided into control group, no-load control group (NC group), and FasL overexpression group (EX group). Osteogenic was induced in each group. Alizarin red staining, real-time PCR, and western blot were used to detect the osteogenic differentiation ability of PDLSCs. Results: The osteogenic differentiation ability of EX group was significantly higher than that of control group and NC group (P<0.05). There was no significant difference between control group and NC group (P>0.05). Conclusion: FasL can promote the osteogenic differentiation of human PDLSCs. References | Related Articles | Metrics

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Experimental Study on Orthodontic Force Response in Periodontal Tissue after Periodontal Regeneration MA Chong, YU Huan, KANG Jian, ZHANG Yuan, TIAN Jing, LI Xinyue 2019, 35(12): 1173-1176.  DOI: 10.13701/j.cnki.kqyxyj.2019.12.018 Abstract ( 170 )   PDF (2687KB) ( 213 )   Objective: To study the effects of orthodontic timing (2 and 12 weeks) on tooth movement speed and periodontal tissue regeneration, and to evaluate the response of periodontal tissue to orthodontic force. Methods: A periodontal bone defect model in Bama miniature pigs was established. Platelet-rich fibrin (PRF) combined with periodontal bone grafting was used to treat bone defect. Orthodontic traction was carried out at 2 and 12 weeks after regeneration. Animals were sacrificed after 6 weeks of orthodontics. Periodontal parameters, micro-CT, histomorphology, and immunohistochemistry were examined. Results: Compared with 12 weeks after regeneration, the orthodontic group at 2 weeks after regeneration had a faster tooth movement speed (P=0.025). Positive correlations of bone mass included higher relative bone volume, number of bone trabecular and thickness of bone trabecular (P=0.019, P=0.014, P=0.024). Negative correlations of bone mass: trabecular segregation, ratio of bone surface area to bone volume, and trabecular mode factor were lower. (P=0.033, P=0.029, P=0.008). Orthodontic bone remodeling was more active, new bone mass was more, and the expression levels of osteoprotegerin and bone morphogenetic protein 2 were higher (P=0.006, P=0.015). Conclusion: Two weeks after periodontal regeneration, the orthodontic teeth move faster, new bone formation is more, and the expressions of osteoprotegerin and osteogenic protein are higher. References | Related Articles | Metrics

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Protective Effect of Low-frequency Magnetic Stimulation on Human Periodontal Ligament Cells Injured by High Glucose ZHENG Guangming, ZHANG Jian, ZHANG Wenyi 2019, 35(12): 1177-1181.  DOI: 10.13701/j.cnki.kqyxyj.2019.12.019 Abstract ( 232 )   PDF (1980KB) ( 185 )   Objective: To investigate the protective effect of low-frequency magnetic stimulation on periodontal membrane injury mediated by high sugar. Methods: Human periodontal membrane cells were cultured by resuscitation in vitro, which were randomly divided into normal control group, high sugar model group, and low-frequency magnetic stimulation group (3 subgroups). Flow cytometry method was used to detect the changes in cell cycle. TUNEL method was used to detect apoptosis. Colorimetric method was used to detect Caspase-3, 6, and 9 activities, and reverse transcription PCR and Western-blot were used to detect CXCL1, CXCL2, and its receptor CXCR2 gene and protein expression levels. Results: Compared with the normal control group, the apoptosis of human periodontal membrane cells increased in the high sugar model. Caspase-3, 6, and 9 activities were all improved (P<0.05). The expressions of CXCL1, CXCL2, and its receptor CXCR2 genes and proteins were significantly reduced (P<0.05). Compared with the high-sugar model group, the cell apoptosis rate in the low-frequency magnetic stimulation group decreased, and the Caspase-3, 6, and 9 activities decreased (P<0.05). CXCL1, CXCL2, and its receptor CXCR2 gene and protein expression increased (P<0.05). The effect on the cell cycle was not obvious (P>0.05). When comparing three subgroups, the change in stimulus intensity above 1.5T was the most obvious (P<0.05). Conclusion: High sugar conditions lead to apoptosis of human periodontal membrane cells, and low-frequency magnetic stimulation has protective effect on human periodontal membrane cell injury induced by high sugar. References | Related Articles | Metrics

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Analysis of Root Surface after Treatments with SRP through Periodontal Endoscope in Vitro LUO Wenqiong, MIAO Lei, PAN Yaping 2019, 35(12): 1182-1186.  DOI: 10.13701/j.cnki.kqyxyj.2019.12.020 Abstract ( 208 )   PDF (5326KB) ( 273 )   Objective: To evaluate the cementum thickness and the root morphology after SRP treatment with or without periodontal endoscope in vitro. Methods: Thirty single root teeth with mobility Ⅲ which need to be extracted were collected and divided into 4 groups randomly: no treatment, Ultra group, Ultra-Hand group, and Ultra-Endo group. Plaque Disclosing Agent was used to measure the residue calculus rate. SEM was applied to observe the surface of teeth. Histology method was taken to measure the thickness of cementum. Results: All three test groups had a much lower calculus residual rate than control group (P<0.05), and both Ultra group and Ultra-Hand group had more calculus residual than Ultra-Endo group (P<0.05). Ultra-Hand group showed a smooth surface with homogeneous patchy distribution and cobblestone appearance with debris. Ultra-Endo group and Ultra group showed roughness, and the cobblestone was the same as the surface of health teeth under EMS. The remaining cementum of Ultra-Endo group was more than those of Ultra group and Ultra-Hand group. Conclusions: Subgingival calculus can be removed more thoroughly and clearly and remain more cementum with the help of periodontal endoscope. References | Related Articles | Metrics

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Study on Relationship between Oxidative Stress and NF-κB-related Inflammatory Factors in Patients with Oral Lichen Planus LI Xin, SUN Yinyin, PAN Yingxiao, WANG Sainan, ZHANG Xinyu, LU Shulai 2019, 35(12): 1187-1190.  DOI: 10.13701/j.cnki.kqyxyj.2019.12.021 Abstract ( 226 )   PDF (749KB) ( 263 )   Objective: To evaluate the oxidative stress status and NF-κB-related inflammatory factors of oral lichen planus patients by saliva samples, and to explore the correlation of them. Methods: Thirty patients with oral lichen planus(the OLP group)and 30 healthy people(the healthy controls)were selected. The OLP group was divided into non-erosive oral lichen planus (NEOLP) group and erosive oral lichen planus (EOLP) group. Indicators of oxidative stress MDA, 8-OHDG, TOS, TAC, and NF-κB related inflammatory factors IL-6, IL-8, and TNF-α were determined by using ELISA. Results: MDA, 8-OHDG, and TOS of OLP group, NEOLP group, and EOLP group were significantly higher than those of healthy people (P<0.05). TAC was significantly lower (P<0.05). The levels of TNF-α, IL-6, and IL-8 in OLP group, NEOLP group, and EOLP group were significantly higher than those in healthy controls (P<0.05). Compared with NEOLP group, MDA and 8-OHDG in EOLP patients increased (P<0.05), while TOC and TOS had no significant difference (P>0.05). There was no significant difference in TNF-α and IL-6 between EOLP patients and EOLP patients (P>0.05), but IL-8 increased significantly (P<0.05). TOS was positively correlated with TNF-α, IL-6, and IL-8 in OLP group, NEOLP group, and EOLP group (P<0.05),while TAC was negatively correlated with TNF-α, IL-6, and IL-8 (P<0.05). Conclusion: There was an increase in oxidative stress and the level of NF-κB related inflammatory factors in OLP patients, and there was a correlation between them. References | Related Articles | Metrics

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Occlusal Reconstruction Treatment of Severely Worn Dentition with CDD/CDM Workflow LIANG Qian, SUN Qianyue, LUO Youcheng, YANG Zinan, WU Zhe 2019, 35(12): 1191-1192.  DOI: 10.13701/j.cnki.kqyxyj.2019.12.022 Abstract ( 294 )   PDF (2383KB) ( 343 )   Completely digital design/completely digital manufacture (CDD/CDM) workflow has been widely used in the restoration of simple dental defects. This article discussed the feasibility of CDD/CDM workflow in the fixed restoration of severely worn dentition. References | Related Articles | Metrics

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A Case of Extensive Subcutaneous Emphysema in Maxillofacial and Neck after High-speed Turbine Tooth Extraction HOU Gaigai, SHANG Zhengjun 2019, 35(12): 1193-1194.  DOI: 10.13701/j.cnki.kqyxyj.2019.12.023 Abstract ( 287 )   PDF (1339KB) ( 400 )   Extensive subcutaneous emphysema in the face and neck after impacted tooth extraction is a relatively rare complication. If it is not handled properly, serious complications may occur. A case of diffuse subcutaneous emphysema of maxillofacial neck and chest after wisdom tooth extraction is reported. References | Related Articles | Metrics