Study on Relationship between Oxidative Stress and NF-κB-related Inflammatory Factors in Patients with Oral Lichen Planus

doi:10.13701/j.cnki.kqyxyj.2019.12.021

Abstract

Abstract: Objective: To evaluate the oxidative stress status and NF-κB-related inflammatory factors of oral lichen planus patients by saliva samples, and to explore the correlation of them. Methods: Thirty patients with oral lichen planus(the OLP group)and 30 healthy people(the healthy controls)were selected. The OLP group was divided into non-erosive oral lichen planus (NEOLP) group and erosive oral lichen planus (EOLP) group. Indicators of oxidative stress MDA, 8-OHDG, TOS, TAC, and NF-κB related inflammatory factors IL-6, IL-8, and TNF-α were determined by using ELISA. Results: MDA, 8-OHDG, and TOS of OLP group, NEOLP group, and EOLP group were significantly higher than those of healthy people (P<0.05). TAC was significantly lower (P<0.05). The levels of TNF-α, IL-6, and IL-8 in OLP group, NEOLP group, and EOLP group were significantly higher than those in healthy controls (P<0.05). Compared with NEOLP group, MDA and 8-OHDG in EOLP patients increased (P<0.05), while TOC and TOS had no significant difference (P>0.05). There was no significant difference in TNF-α and IL-6 between EOLP patients and EOLP patients (P>0.05), but IL-8 increased significantly (P<0.05). TOS was positively correlated with TNF-α, IL-6, and IL-8 in OLP group, NEOLP group, and EOLP group (P<0.05),while TAC was negatively correlated with TNF-α, IL-6, and IL-8 (P<0.05). Conclusion: There was an increase in oxidative stress and the level of NF-κB related inflammatory factors in OLP patients, and there was a correlation between them.

Key words: oral lichen planus, oxidative stress, NF-κB

LI Xin, SUN Yinyin, PAN Yingxiao, WANG Sainan, ZHANG Xinyu, LU Shulai. Study on Relationship between Oxidative Stress and NF-κB-related Inflammatory Factors in Patients with Oral Lichen Planus[J]. Journal of Oral Science Research, 2019, 35(12): 1187-1190.

References

[1] 陈谦明. 主编. 口腔黏膜病学[M]. 第4版. 北京:人民卫生出版社, 2013: 103-110.
[2] Alrashdan MS, Cirillo N, Mccullough M. Oral lichen planus:a literature review and update [J]. Arch Dermatol Res, 2016, 308(8): 539-551.
[3] Buczko P, Zalewska A, Szarmach I, et al. Saliva and oxidative stress in oral cavity and in some systemic disorders [J]. J Physiol Pharmacol, 2015, 66(1): 3-9.
[4] Kattoor AJ, Pothineni NV, Palagiri D, et al. Oxidative stress in atherosclerosis [J]. Curr Atheroscler Rep, 2017, 19(11): 42.
[5] 邹雅丹, 刘玮, 石连杰, 等. 氧化应激在系统性红斑狼疮中的研究进展[J]. 中华医学杂志, 2018,98(27):2213-2216.
[6] Ergun S, Trosalaş C, Warnakulasuriya S, et al. Evaluation of oxidative stress and antioxidant profile in patients with oral lichen planus [J]. J Oral Pathol Med, 2011, 40(4): 286-293.
[7] 满昭昭, 许野, 朱兰兰, 等. 核因子-κB与肿瘤坏死因子α、白介素1β在口腔扁平苔藓中的表达及意义[J]. 口腔医学, 2013, 33(7): 461-464.
[8] Lugrin J, Rosenblatt-Velin N, Parapanov R, et al. The role of oxidative stress during inflammatory processes [J]. Biol Chem, 2014, 395(2): 203-230.
[9] Tvarijonaviciute A, Aznar-Cayuela C, Rubio CP, et al. Evaluation of salivary oxidate stress biomarkers, nitric oxide and C-reactive protein in patients with oral lichen planus and burning mouth syndrome [J]. J Oral Pathol Med, 2017, 46(5):387-392.
[10] Tóthová L, Kamodyová N, cervenka T, et al. Salivary markers of oxidative stress in oral diseases [J]. Front Cell Infect Microbiol, 2015, 5: 73.
[11] Totan A, Miricescu D, Parlatescu I, et al. Possible salivary and serum biomarkers for oral lichen planus [J]. Biotech Histochem, 2015, 90(7): 552-558.
[12] 中华口腔医学会口腔粘膜病专业委员会.口腔扁平苔藓(萎缩型、糜烂型)疗效评价标准(试行)[J].中华口腔医学杂志,2005,40(2):92-93.
[13] 中华口腔医学会口腔黏膜病专业委员会,中华口腔医学会中西医结合专业委员会.口腔扁平苔藓诊疗指南(试行)[J].中华口腔医学杂志,2012,47(7):399-401.
[14] Ergun S, Troşala SC, Warnakulasuriya S, et al. Evaluation of oxidative stress and antioxidant profile in patients with oral lichen planus [J]. J Oral Pathol Med, 2011, 40(4): 286-293.
[15] 董子昱, 张芳. 口腔扁平苔藓唾液标记物研究现状分析[J]. 口腔医学研究, 2018, 34(11): 1168-1171.
[16] Scrobotă I, Mocan T, Cătoi C, et al. Histopathological aspects and local implications of oxidative stress in patients with oral lichen planus [J]. Rom J Morphol Embryol, 2011, 52(4): 1305-1309.
[17] Sezer E, Ozugurlu F, Ozyurt H, et al. Lipid peroxidation and antioxidant status in lichen planus [J]. Clin Exp Dermatol, 2007, 32(4): 430-434.
[18] Agha-Hosseini F, Mirzaii-Dizgah I, Mikaili S, et al. Increased salivary lipid peroxidation in human subjects with oral lichen planus [J]. Int J Dent Hyg, 2009, 7(4): 246-250.
[19] Abdolsamadi H, Rafieian N, Goodarzi MT, et al. Levels of salivary antioxidant vitamins and lipid peroxidation in patients with oral lichen planus and healthy individuals [J]. Chonnam Med J, 2014, 50(2): 58-62.
[20] 李勇伟, 邱勋定, 顾明, 等. 同源形成素样蛋白2基因对口腔鳞状细胞癌侵袭、迁移及核因子-κB信号的影响[J]. 口腔医学研究, 2019, 35(2):137-141.
[21] Wang Y, Zhang H, Du G, et al. Total glucosides of paeony (TGP) inhibits the production of inflammatory cytokines in oral lichen planus by suppressing the NF-κB signaling pathway [J]. Int Immunopharmacol, 2016, 36: 67-72.
[22] Verma A, Singh D, Anwar F, et al. Triterpenoids principle of Wedelia calendulacea attenuated diethynitrosamine-induced hepatocellular carcinoma via down-regulating oxidative stress, inflammation and pathology via NF-κB pathway [J]. Inflammopharmacology, 2018, 26(1): 133-146.
[23] Darczuk D, Kraysciak W, Vyhouskaya P, et al. Salivary oxidative status in patients with oral lichen planus [J]. J Physiol Pharmacol, 2016, 67(6): 885-894.