Effect of FMNL2 Gene on Invasion, Migration, and NF-κB Signal in Oral Squamous Cell Carcinoma.

doi:10.13701/j.cnki.kqyxyj.2019.02.009

Abstract

Abstract: Objective : To investigate the effect of down-regulation of FMNL2 gene expression on invasion, migration, and NF-κB signal in oral squamous cell carcinoma (OSCC). Methods: FMNL2 specific siRNA (si-FMNL2) and negative control siRNA (NC) were transfected into human oral squamous cell carcinoma cells CAL-27. PDTC was set as NF-κB signal inhibitor. After the cells were treated for 48h, western blotting was used to detect FMNL2, E-cadherin, Vimentin, NF-κBp65, and IκBα protein expression. Results: Compared with the blank group, the cell invasion and migration ability in si-FMNL2 group and PDTC group decreased obviously, the expression of E-cadherin protein increased obviously, and the expression of Vimentin, NF-κBp65, and IκBα protein decreased significantly (P<0.05). Compared with the PDTC group, the cell invasion and migration ability in PDTC+si-FMNL2 group decreased obviously, the expression of E-cadherin protein increased obviously, and the expression of Vimentin protein decreased significantly (P<0.05). Conclusion: Downregulation of FMNL2 gene expression can reverse EMT by inhibiting NF-κB signaling pathway, thus inhibiting the invasion and migration of oral squamous cell carcinoma.

Key words: Oral squamous cell carcinoma, FMNL2 gene, Invasion, Migration, EMT, NF-κB signal

LI Yong-wei, QIU Xun-ding, GU Ming, DENG Yi, XIE Li-li. Effect of FMNL2 Gene on Invasion, Migration, and NF-κB Signal in Oral Squamous Cell Carcinoma.[J]. Journal of Oral Science Research, 2019, 35(2): 137-141.

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