Journal of Oral Science Research

Journal of Oral Science Research ›› 2019, Vol. 35 ›› Issue (2): 142-146.DOI: 10.13701/j.cnki.kqyxyj.2019.02.010

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Expression of Homo Sapiens Longevity Assurance Homologue 2 of Yeast LAG1 in Oral Squamous Cell Carcinoma and Its Effect on Cell Proliferation and Invasion.

WANG Xiao-long1, CAO Shun-shun1, ZHANG Peng1, SHU Chuan-ji1, XIA Lin2*   

  1. 1. Department of Stomatology, Edong Medical Group Huangshi Central Hospital, Affiliated Hospital of Hubei Institute of Technology, Huangshi 435000, China;
    2. Department of Head and neck oncology, Edong Medical Group Huangshi Central Hospital, Affiliated Hospital of Hubei Institute of Technology, Huangshi 435000, China.
  • Received:2018-08-21 Online:2019-02-18 Published:2019-02-25

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Abstract: Objective : To investigate the expression of homo sapiens longevity assurance homologue 2 of yeast LAG1 (LASS2) in oral squamous cell carcinoma, and explore the effect of silencing LASS2 gene expression on proliferation and invasion of CAL27 cells. Methods: A total of 64 cases of patients with oral squamous cell carcinoma were selected. The expressions of LASS2 gene and protein in oral squamous cell carcinoma and adjacent tissues were detected by using real-time quantitative PCR and Western blot. CAL-27 cells were cultured and divided into siRNA-LASS2 group, negative control group, and blank group. The expression of LASS2 gene in cells was detected by using real-time quantitative PCR. The cell proliferation was detected by using MTT assay. The cell migration was detected by using scratch test. The cell invasive was detected by using Transwell chamber. Results: The relative expression levels of LASS2 mRNA and protein in the oral squamous cell carcinoma tissues were lower than those in the adjacent tissues (P<0.05). The relative expression levels of LASS2 mRNA and protein in oral squamous cell carcinoma tissues were related to TNM stage, differentiation degree, clinical stage, and lymph node metastasis (P<0.05). The relative expression level of LASS2 mRNA in the siRNA-LASS2 group was lower than those in the negative control group and the blank group (P<0.05). The absorbance A values at 1d, 2d, 3d, and 4d in the siRNA-LASS2 group were higher than those in the negative control group and the blank group (P<0.05). The cell migration rates at 24h and 48h in the siRNA-LASS2 group were higher than those in the negative control group and the blank group (P<0.05). The number of invasive cells in the siRNA-LASS2 group was higher than that in the negative control group and the blank group (P<0.05). Conclusion: LASS2 was lowly expressed in oral squamous cell carcinoma tissues. Specific inhibition of LASS2 expression in oral squamous cell carcinoma cells could promote cell proliferation and accelerate cell migration and invasion.

Key words: Oral squamous cell carcinoma, Homo sapiens longevity assurance homologue 2 of yeast LAG1 Cell proliferation, Invasiveness