Effect of Human Umbilical Cord Mesenchymal Stem Cells on Invasion and Migration of Cal-27 Cells from Tongue Squamous Cell Carcinoma.

doi:10.13701/j.cnki.kqyxyj.2019.05.008

Journal of Oral Science Research ›› 2019, Vol. 35 ›› Issue (5): 443-447.DOI: 10.13701/j.cnki.kqyxyj.2019.05.008

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Effect of Human Umbilical Cord Mesenchymal Stem Cells on Invasion and Migration of Cal-27 Cells from Tongue Squamous Cell Carcinoma.

ZHANG Xin-yu^1#, WU Shuang-yan^2#, WU Hong¹, XU Zhu-qing¹, WANG Yun-ying³, XU Xiao-na³, LI Xin¹, ZHENG Jian-jin^1*

1. School of Stomatology of Qingdao University, Qingdao 266003, China;
2. Qingdao Stomatological Hospital, Qingdao 266001, China;
3. Central Laboratory of Qingdao Municipal Hospital, Qingdao 266000, China.

Received:2018-09-21 Online:2019-05-28 Published:2019-05-21

Abstract

Abstract: Objective: To investigate the effect of human umbilical cord mesenchymal stem cells (HUC-MSCs) on the invasion and migration of Cal-27 cells in tongue squamous cell carcinoma. Methods: HUC-MSCs conditioned medium was prepared. Cal-27 cells were cultured and the effect of HUC-MSCs secreted factors on Cal-27 cell migration was detected by cell scratch test. The invasiveness and migration of Cal-27 cells before and after co-culture were compared in Transwell cells. Expression levels of genes related to invasion and migration of Cal-27 cells were detected by RT-PCR before and after co-culture. The changes of MMP2 and MMP9 protein expression in tumor invasion and metastasis-related protein molecules were detected by western blot. Results: The migration distance of Cal-27 cells was shorter than that of the control group. After co-culture, the number of invasion and migration of Cal-27 cells decreased. The expression levels of aggression-related genes and proteins were down-regulated. Conclusion: The co-culture of HUC-MSCs and Cal-27 cells can inhibit the invasion and migration of Cal-27 cells.

Key words: Human umbilical cord mesenchymal stem cells, Tongue squamous cell carcinoma Cal-27, Conditioned medium, Invasion and migration, Matrix metalloproteinases

ZHANG Xin-yu, WU Shuang-yan, WU Hong, XU Zhu-qing, WANG Yun-ying, XU Xiao-na, LI Xin, ZHENG Jian-jin. Effect of Human Umbilical Cord Mesenchymal Stem Cells on Invasion and Migration of Cal-27 Cells from Tongue Squamous Cell Carcinoma.[J]. Journal of Oral Science Research, 2019, 35(5): 443-447.

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Effect of Human Umbilical Cord Mesenchymal Stem Cells on Invasion and Migration of Cal-27 Cells from Tongue Squamous Cell Carcinoma.

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