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28 May 2019, Volume 35 Issue 5 Previous Issue    Next Issue

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Application of 125I Radioactive Seed Implantation in Treatment of Oral and Maxillofacial Malignant Tumors. SUN Jin-hu, LI Meng 2019, 35(5): 415-418.  DOI: 10.13701/j.cnki.kqyxyj.2019.05.001 Abstract ( 239 )   PDF (874KB) ( 241 )   How to improve the cure rate and survival rate of oral and maxillofacial malignant tumor, and maintain a higher quality of life, have been one of the focus of the scholars study. Radical resection of oral and maxillofacial malignant tumor often leads to severe facial deformity and dysfunction, such as oral cavity, so now the chemotherapy or radiotherapy combined with surgical treatment is often used. In recent years, studies have shown that close range radiotherapy with 125I radioactive seed implantation is effective in reducing tumor size and reducing recurrence of oral and maxillofacial malignant tumors. In this paper, the clinical application of 125I radioactive seed in the treatment of oral and maxillofacial malignant tumors is described. References | Related Articles | Metrics

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Research Progress on Relationship between Chronic Periodontitis and Alzheimer’s Disease. NIE Ran, ZHOU Yan-min 2019, 35(5): 419-422.  DOI: 10.13701/j.cnki.kqyxyj.2019.05.002 Abstract ( 369 )   PDF (768KB) ( 290 )   Chronic periodontitis is a destructive disease of dental support tissues which originated by dental plaque and generated by host immune-inflammatory responses and other factors. Its occurrence and development are closely associated with general systemic disease. Alzheimer's disease is one of the most common progressive neurocognitive disorder diseases. Recently, studies have found that chronic periodontitis is mutually associated with Alzheimer's disease. In this review, we conclude the correlation between chronic periodontitis and Alzheimer's disease, and summarize the mechanisms how chronic periodontitis participates in and promotes the progression of Alzheimer's disease. References | Related Articles | Metrics

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Research Progress of Traditional Chinese Medicine in Treatment of Oral Submucous Fibrosis. GUO Jin-cai, XIE Hui 2019, 35(5): 423-426.  DOI: 10.13701/j.cnki.kqyxyj.2019.05.003 Abstract ( 318 )   PDF (756KB) ( 523 )   Oral submucous fibrosis (OSF) mainly focuses on drug therapy. Traditional Chinese medicine has made a great progress in the treatment of the disease in recent years. This paper firstly explains the mechanism of OSF, and then summarizes the clinical research progress in recent years from natural medicines, proprietary Chinese medicine, and prescriptions, with aim to provide reference for clinical medication of OSF. References | Related Articles | Metrics

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Research Progress of Idiopathic Condylar Resorption. CHENG Hui-na, ZHOU Qing 2019, 35(5): 427-429.  DOI: 10.13701/j.cnki.kqyxyj.2019.05.004 Abstract ( 341 )   PDF (746KB) ( 430 )   Idiopathic condylar resorption is a serious facial deformity caused by unexplained resorption of condylar, which led to progressive tooth opening and mandibular retrusion. The main clinical manifestations are early posterior teeth contact and type Ⅱ malocclusion. Patients often seek medical treatment because of progressive occlusion of anterior teeth. The facial morphology usually changes during the treatment, which greatly affects the life quality of the patients. The treatment methods and prognosis are controversial, so people pay more and more attention in recent years. In this paper, the possible etiology, pathogenesis, clinical diagnosis, and treatment of the disease are systematically reviewed so as to provide the basis for further research on the special condyle absorption. References | Related Articles | Metrics

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Understanding and Controversy of Mandibular Idiopathic Condylar Resorption. XU Fan, ZHOU Qing 2019, 35(5): 430-432.  DOI: 10.13701/j.cnki.kqyxyj.2019.05.005 Abstract ( 268 )   PDF (745KB) ( 306 )   Idiopathic condylar resorption is a disease characterized with progressive condylar resorption of unknown origin. However, the understanding of ICR is still in infancy. In this article, we will review a lot of literature and discuss the understanding and controversy of ICR from the definition, etiology and pathology, diagnosis and differential diagnosis, and treatment. References | Related Articles | Metrics

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Significance of TGF-β1 Expression and Clinicopathological Characteristics on Prognosis for Oral Squamous Cell Carcinoma Patients. LU Zhan-yi, HU Qin-gang, CHEN Sheng, YANG Yan, DING Liang, NI Yan-hong 2019, 35(5): 433-438.  DOI: 10.13701/j.cnki.kqyxyj.2019.05.006 Abstract ( 235 )   PDF (4588KB) ( 320 )   Objective: To investigate transforming growth factor beta1 (TGF-β1) expression at tumor center and invasive tumor front and the prognostic value in oral squamous cell carcinoma (OSCC). Methods: Paraffin-embedded sections of 104 patients with primary OSCC were collected at Nanjing Stomatological Hospital. The sections were stained by immunohistochemistry (IHC) and focused on regions at tumor center (TC) and invasive tumor front (ITF). Results: Positive expression rates of TGF-β1 between TC and ITF were comparable (P>0.05). Moreover, TGF-β1 expression at TC and ITF positively associated with TNM stages, DOI, nerve invasion and recurrence (P<0.05). Kaplan-Meier analysis showed that high expression of TGF-β1 no matter at TC or ITF was significantly correlated with lower overall survival, recurrence-free survival, and disease-free survival (P<0.05). Notably, Cox multivariate analysis indicated that high expression of TGF-β1 at TC correlated to low overall survival in OSCC patients. Conclusion: TGF-β1 at TC could be an independent prognostic factor for overall survival in OSCC patients. References | Related Articles | Metrics

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Expression and Clinical Significance of SOX-9 in Human Oral Squamous Cell Carcinoma. LIU Yan-ling, ZHAO Xi, YU Li, NIE Min-hai 2019, 35(5): 439-442.  DOI: 10.13701/j.cnki.kqyxyj.2019.05.007 Abstract ( 237 )   PDF (1935KB) ( 489 )   Objective: To investigate the expression and clinical significance of SOX-9 in oral squamous cell carcinoma (OSCC). Methods: The expression of SOX-9 protein in normal oral mucosa, atypical hyperplasia of oral epithelium, and oral squamous cell carcinoma was detected by immunohistochemistry, and the correlation between SOX-9 protein and clinicopathological factors was analyzed. Results: The positive expression rates of SOX-9 in normal oral mucosa, atypical epithelial hyperplasia, and oral squamous cell carcinoma were 10% (2/20), 32.1% (9/28), and 79.4% (27/34), respectively. SOX-9 increased in three tissues in turn. The expression of SOX-9 increased in the process of tumor development, and the difference of expression in each group was statistically significant (P<0.001). The expression of SOX-9 was related to tumor volume, lymph node metastasis, and tumor stage. Single factor analysis showed that tumor volume, lymph node metastasis , tumor staging, and SOX-9 positive expression were related to survival time (P<0.05). After Cox multivariate analysis, only lymph node metastasis, tumor stage, and SOX-9 positive expression were independent factors affecting survival time (P<0.05). Conclusions: SOX-9 is highly expressed in oral squamous cell carcinoma. SOX-9 expression is associated with survival rate in patients with oral squamous cell carcinoma. References | Related Articles | Metrics

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Effect of Human Umbilical Cord Mesenchymal Stem Cells on Invasion and Migration of Cal-27 Cells from Tongue Squamous Cell Carcinoma. ZHANG Xin-yu, WU Shuang-yan, WU Hong, XU Zhu-qing, WANG Yun-ying, XU Xiao-na, LI Xin, ZHENG Jian-jin 2019, 35(5): 443-447.  DOI: 10.13701/j.cnki.kqyxyj.2019.05.008 Abstract ( 208 )   PDF (2211KB) ( 259 )   Objective: To investigate the effect of human umbilical cord mesenchymal stem cells (HUC-MSCs) on the invasion and migration of Cal-27 cells in tongue squamous cell carcinoma. Methods: HUC-MSCs conditioned medium was prepared. Cal-27 cells were cultured and the effect of HUC-MSCs secreted factors on Cal-27 cell migration was detected by cell scratch test. The invasiveness and migration of Cal-27 cells before and after co-culture were compared in Transwell cells. Expression levels of genes related to invasion and migration of Cal-27 cells were detected by RT-PCR before and after co-culture. The changes of MMP2 and MMP9 protein expression in tumor invasion and metastasis-related protein molecules were detected by western blot. Results: The migration distance of Cal-27 cells was shorter than that of the control group. After co-culture, the number of invasion and migration of Cal-27 cells decreased. The expression levels of aggression-related genes and proteins were down-regulated. Conclusion: The co-culture of HUC-MSCs and Cal-27 cells can inhibit the invasion and migration of Cal-27 cells. References | Related Articles | Metrics

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Effect of anti-Streptococcus Mutans Yolk Antibody on Virulence Factors and Plaque Biofilm induced by Streptococcus Mutans. ZHOU Li-li, XU Yan, JIANG Peng, XU HAN-ying, XIN Bao-jian, SHEN Ji-long, CHENG Ting 2019, 35(5): 448-452.  DOI: 10.13701/j.cnki.kqyxyj.2019.05.009 Abstract ( 289 )   PDF (2509KB) ( 248 )   Objective: The anti-Streptococcus mutans egg yolk immunoglobulin (anti-S. mutans-IgY) was prepared and purified, and its effect on the extracellular polysaccharides capacity, glucosyltransferase activity, biofilm formation and activity of S. mutans was observed. Methods: Ten 150 day-old laying hens were immunized with S. mutans and IgY was isolated from the egg yolk by the water dilution (WD) method and 2 steps of ammonium sulfate purification. ELISA was used to detect changes in titer after immunization. The anthrone method was used to observe the water soluble glucan (WSG) and water insoluble glucan (WIG), the Neson-Somogyi method was used to determine the activity of GTF enzyme, and the XTT method was used to study the effect of IgY on the total amount of biofilm. Results: After 4 immunizations, the highest titer of the antibody was 1:256000, which was maintained for 40 days. IgY inhibited the WIG synthesis of S.mutans, and decreased the S.mutans reducing sugar and enzyme activity (P<0.01). The XTT method showed that the inhibition rate of IgY to S. mutans biofilm was17.02%-74.13%. Microscopy showed that the green viable bacteria and structure decreased and the biofilm formation was significantly inhibited as the concentration of the drug solution increased. Conclusion: IgY can inhibit the proliferation of S. mutans and has an anti-caries effect. References | Related Articles | Metrics

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Effects of Rein Infiltration and Bioactive Glass on Early Caries. DAI Jian-mei, LI Feng-hua, Qiu Xi-li, DONG Xin 2019, 35(5): 453-456.  DOI: 10.13701/j.cnki.kqyxyj.2019.05.010 Abstract ( 256 )   PDF (1403KB) ( 196 )   Objective: To study the effects of combined application of rein infiltration and bioactive glass on enamel caries. Method: 51 extracted human teeth prepared as artificial incipient enamel caries were randomly divided into 5 groups. They were treated with resin infiltration (group A), 0.6% bioactive glass (group B), 0.1% sodium fluoride solution (group C), 0.6% bioactive glass solution (group D), and blank control group (E group) for 4 weeks. Scanning electron microscope observation and surface microhardness measure were made before and after demineralization. Result: SEM observation showed that the surface porosity of A-D group was significantly reduced after 4 weeks treatment, and surface smoothness of group D was the best. After treatment, the values of ΔSMH1 ranged from large to small in the order of D, C, A, B, E. There was significant difference in the ΔSMH1 values between groups except group A and group B. Conclusion: The resin infiltration combined with bioactive glass could effectively repair early enamel caries. References | Related Articles | Metrics

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Electron Microscopic Observation on Bonding Interface between Nano-hydroxyapatite Composite Resin and Enamel. WANG Zhi-ping, ZHAO Meng-meng, DONG Zhi-heng, NIU Yue-yue, WANG Qian, WANG Qingshan 2019, 35(5): 457-460.  DOI: 10.13701/j.cnki.kqyxyj.2019.05.011 Abstract ( 201 )   PDF (1445KB) ( 269 )   Objective: To analyze and compare the fitness of material and teeth through observing the bonding interface between nano hydroxyapatite composite resin and enamel. Methods: 30 healthy first molars were prepared and randomly divided into three groups: group A, group B, and group C. Group A was filled with new nano-hydroxyapatite composite resin. Group B was filled with Karisma composite resin, and group C with glass ionomer cement. After the cold and hot cycle aging test, the teeth and material filling position was cut by low speed Emery slice under flowing water, which was then polished into a thin slice. The bonding interface between the filling material and enamel was observed under scanning electron microscope and the gap was measured. Results: The interfacial gap between nano-hydroxyapatite composite resin and enamel was small, the gap between Karisma composite resin and enamel was larger, and the gap between glass ionomer cement and enamel was the largest. There was significant difference among the three groups (P<0.001). Conclusion: The new nano-hydroxyapatite composite resin has good adhesion to enamel. References | Related Articles | Metrics

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Comparison of Inflammatory Factors and Immune Function between HIV-infected and Non-HIV-infected Patients with Chronic Apical Periodontitis. ZHAO Heng-yue, HE Wen-juan 2019, 35(5): 461-465.  DOI: 10.13701/j.cnki.kqyxyj.2019.05.012 Abstract ( 196 )   PDF (3832KB) ( 211 )   Objective: To compare the differences of inflammatory factors and immune function between HIV-infected and non-HIV-infected patients with chronic apical periodontitis. Methods: The excised pathological tissues were obtained from the teeth of 48 patients (24 HIV-infected patients and 24 non-HIV-infected patients) with chronic apical periodontitis. No patients were treated with preoperative endodontic treatment. The samples were histopathologically and immunohistochemically analyzed by an optical microscope. The immune expression was divided into 2 levels: focal to weak and moderate to strong. Results: The expressions of CD3, CD4, CD8, CD68, IFN-g, IL-6, and IL-18 in HIV-infected group were lower than those in non-HIV-infected group, and the expression of TNF-α in infected group was higher than that in non-HIV-infected group (P<0.05). There was no significant difference in CD20 expression between two groups (P>0.05). Conclusion: HIV infection has an effect on the expression of inflammatory factors and immune function in patients with chronic apical periodontitis. References | Related Articles | Metrics

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Preliminary Study on Effect of Yolk Antibody against Prevotella Intermedia on Gingivitis during Pregnancy in Rats. JIANG Peng, XU Yan, ZHOU Li-li, XU Han-ying, XIN Bao-jian, YE Xin-ru, SHEN Ji-long, CHENG Ting 2019, 35(5): 466-470.  DOI: 10.13701/j.cnki.kqyxyj.2019.05.013 Abstract ( 244 )   PDF (2105KB) ( 190 )   Objective: To prepare and purify specific yolk immunoglobulin (IgY) against Prevotella intermedia(P.intermedia, Pi)and to evaluate its therapeutic effect on experimental gingivitis in pregnant rats. Methods: Specific IgY was prepared by two-step ammonium sulfate precipitation method. Twenty-four female SD rats were randomly divided into 4 groups: group A (blank control group), group B (negative group): modeled, group C (positive control group): modeled + tinidazole solution, and group D (experimental group): modeled + specific IgY solution. The therapeutic effect of specific IgY was observed by gingival index (GI), plaque index (PLI), body weight change, RT-qPCR, and histopathological sections. Results: Through the treatment of experimental gestational gingivitis in rats, the GI, PLI (P<0.001) and body weight (P<0.05) were improved, and the composition ratio of P. intermedia was significantly decreased after the treatment of subgingival plaque (P<0.05). Conclusion: Anti-P.intermedia--specific yolk antibody can relief the gingival inflammation in rats during pregnancy. References | Related Articles | Metrics

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Effect of β-cryptoxanthin on Level of Inflammatory Factors in Experimental Periodontitis. LI Dong-xue, WANG Guo-fang, CUI Xiao-yu, LI Xiao-hong, XUAN Ya-ru, WANG Shou-ru 2019, 35(5): 471-475.  DOI: 10.13701/j.cnki.kqyxyj.2019.05.014 Abstract ( 239 )   PDF (2332KB) ( 177 )   Objective: To study the effect of β-cryptoxanthin on inflammatory factors in rats with experimental periodontitis and to explore its mechanism. Methods: 30 male Sprague-Dawley rats were randomly divided into normal group (group N), periodontitis group (group P), and β-cryptoxanthin intervention group (group E), with 10 rats of each. Experimental periodontitis model was induced by ligation of bilateral maxillary second molars cervix and injection with lipopolysaccharide (LPS). At the same time, β-cryptoxanthin (12μl/each) was injected in group E at the same site. All groups were treated every 48 hours for three times. Blood was collected and the bilateral maxilla were immediately separated on day 7, the right part of the tissues were subjected to histological analysis, serum and the left gingival tissues were detected by ELISA for IL-1β, TNF-α, and PGE2. Results: The levels of IL-1β, TNF-α and PGE2 in group E and group P were higher than those in group N, the infiltration of inflammatory cells was increased and the alveolar bone absorption was obvious. Compared with group P, the levels of IL-1β, TNF-α, and PGE2 in group E were significantly lower, the infiltration of inflammatory cells was less, and the absorption of alveolar bone was mild. Conclusion: β-cryptoxanthin can decrease the level of inflammatory factors and the inflammatory cells infiltration in tissues, thereby reducing the inflammation of periodontaltissues and alleviating alveolar bone resorption. References | Related Articles | Metrics

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Clinical Observation of Gingival Shaping by Temporary Crown during Second-stage Surgery of Implantation. LIU Xiao-qing, ZHOU Hong 2019, 35(5): 476-479.  DOI: 10.13701/j.cnki.kqyxyj.2019.05.015 Abstract ( 276 )   PDF (1733KB) ( 223 )   Objective: To evaluate the correlation between the esthetic score and the satisfaction of the patients by temporary crown gingival shaping performed during the maxillary anterior single-tooth implant second-stage surgery. Methods: 30 patients with single anterior teeth loss were selected according to the inclusion criteria before surgery. The temporary crown was used to shape the gingiva during the second-stage surgery. Permanent restoration was completed after 3-6 months. The pink esthetic score (PES) and white esthetic score (WES) were used for aesthetically evaluation 1 year after permanent restoration and the visual analog scale (VAS) was used for subjective satisfaction of patients. The correlation between PES and WES, VAS and PES, and VAS and WES was analyzed. Results: The average score of PES and WES were (8.03+0.85) and (8.10+0.40). PES value and WES value were significantly positive correlated (r=0.90,P<0.01). The average score of VAS was (8.68+0.58). VAS was significantly positive correlated with PES value and WES value (r=0.93,P<0.01; r=0.81, P<0.01). Conclusion: Permanent restoration of the maxillary anterior single-tooth implant with temporary crown shaping during second-stage surgery can achieve good aesthetic effect and high satisfaction. There is a high correlation between PES and WES, VAS and PES, and VAS and WES. References | Related Articles | Metrics

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Effect of Different Loading Time on Osseointegration between Mini-implant and Bone under Large Loading Force. QIU Yu-bei, GUO Wei-zhong, CHEN Jiang 2019, 35(5): 480-482.  DOI: 10.13701/j.cnki.kqyxyj.2019.05.016 Abstract ( 199 )   PDF (924KB) ( 181 )   Objective: To investigate the effect of different loading time on the osseointegration between mini-implant and bone under large loading force. Methods: Twenty-four mini-implants were implanted into the mandibles of six beagle dogs. 350g force was loaded onto the corresponding mini-implants immediately (0 w group), 2 weeks (2w group), 4 weeks (4w group), and 8 weeks (8w group) after implantation. Maximum removal torque (MRT) was tested and histology of the interface of implant-bone tissue was observed using HE staining after 4 weeks of loading. Results: Two of the mini-implants in 2w group were lost and 1 in 0w group was unstable. Other mini-implants were stable. The MRT of the 8w group was higher than that of 4w group (P=0.03). The interface of mini-implant-bone tissue in 8w group was composed of mature bone tissue, while it was composed of newly formed bone in 4w group. The lowest MRT was detected from the 0w group and 2w group, and no significant difference was found between these two groups (P=0.78). Furthermore, more obvious fibrous tissue was observed in the 2w group in contrast to 0w group. Conclusion: Some risks may occur if loaded immediately or 2 weeks after implantation. It is suggested to load 4 weeks later after implantation. References | Related Articles | Metrics

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Effect of TGF-β1 Gene Modified Bone Marrow Stromal Cells Combined with Bio-Oss Collagen on Repairing Canine Jaw Defects. GUO Hong-liang, ZHENG Ji-wei, WANG Peng-lai, LIU Zong-xiang, YUAN Chang-yong, LI Gan, LI Xiao-fei, GENG Xiao-qing 2019, 35(5): 483-487.  DOI: 10.13701/j.cnki.kqyxyj.2019.05.017 Abstract ( 210 )   PDF (1102KB) ( 177 )   Objective: To observe the effect of TGF-β1 gene modified Bone Marrow Stromal Cells (BMSC) combined with Bio-Oss collagen on jaw defects reconstruction. Methods: 18 beagle dogs were randomly divided into three groups. Control group: implant+BMSC+gelatin+Bio-Oss collagen group A: implant+BMSC transfected with 0.5 μg/L TGF-β1+gelatin+Bio-Oss collagen; group B: implant+BMSC transfected with 10 μg/L TGF-β1+gelatin+Bio-Oss collagen. Histological view was applied to measure the repair effect. Meanwhile, the mRNA and protein expressions of OC, BSP and COL-I were detected through RT-PCR and Western blot, respectively. Results: HE staining showed that bone continuity and maturity in group A were superior to those in group B. RT-PCR results showed that the expressions of mRNA in the experimental group A were significantly higher than those in the control group (P<0.05). However, compared with the control group, the mRNA expression levels in the experimental group B were not statistically significant (P>0.05). In addition, Western blot results showed that expressions of OC, BSP, and COL-I protein in the group A were significantly higher than those of control group and group B (P<0.05), while that of group B was slightly higher than that of control group, but without statistically significant (P>0.05). Conclusion: 0.5 μg/L TGF-β1 gene has a better repair effect on promoting osteogenic differentiation of BMSC. References | Related Articles | Metrics

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Effects of Bone Morphogenetic Protein Receptor 2 on Ameloblastic Differentiation of Induced Pluripotent Stem Cells. LIU Zhi, QIN Qing, GAO Peng, SUN Cong, ZHANG Yue-qi 2019, 35(5): 488-492.  DOI: 10.13701/j.cnki.kqyxyj.2019.05.018 Abstract ( 192 )   PDF (2118KB) ( 165 )   Objective: To investigate the effects of bone morphogenetic protein receptor 2 (BMPR-Ⅱ) on the ameloblastic differentiation of induced pluripotent stem cells (iPS). Methods: The mouse embryo fibroblasts treated with mitomycin-C were used as the feeder cells for iPS cells. Real-time PCR and western blot were used to measure the gene and protein expression of different BMP receptors by iPS cells cultured with control medium or ASF-CM. Immunohistochemical staining and flow cytometry were used to detect the expression of ameloblastin, enamelin, and cytokeratin-14 by BMPR-Ⅱ siRNA-pretreated iPS cells cultured with control medium or ASF-CM. Results: The gene and protein expression of BMPR-Ⅱ by iPS cells treated with ASF-CM were significantly higher than those of the controls (P<0.05). ASF-CM treatment significantly increased the gene and protein expression of ameloblastin, enamelin, and cytokeratin-14 (P<0.05), which could be obviously reversed by BMPR-Ⅱ siRNA pre-treatment (P<0.05). Conclusion: BMPR-Ⅱ plays an important role during the regulation of the ameloblastic differentiation of iPS cells. References | Related Articles | Metrics

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Study on Transdifferentiation of Adipose-derived Stem Cells into Salivary Gland Acinar-like Cells. YU Yang, XU Wen-pin, JIAO Kun, LIU Dong-rui, XING Ru-xiao, YE Jia-peng, ZHANG Wei 2019, 35(5): 493-496.  DOI: 10.13701/j.cnki.kqyxyj.2019.05.019 Abstract ( 185 )   PDF (2004KB) ( 274 )   Objective: To investigate whether adipose-derived stem cells (ADSCs) can be transformed into acinar-like cells with secretory function in the microenvironment simulated by submandubular gland cells (SMGCs). Methed: ADSCs were isolated from neonatal SD rats and cultured in vitro. Submandibular gland cell lysate was used to induce differentiation. To observe the morphological changes and expression of α-amylase antibody and cytokeratin-8 antibody, the content of amylase in the supernatant of cells induced at different periods was detected using amylase detection kit, and the conversion efficiency was analyzed. Results: The cells extracted from adipose tissue of rats had the ability to differentiate into multiple directions, which confirmed that they were adipose derived stem cells. After induced by submandibular gland cell lysate, α-amylase antibody and cytokeratin-8 antibody staining were positive, but negative in control group. This confirmed that the cells were successfully transformed into acinar-like cells, and the content of amylase in the supernatant increased with time, which indicated that the induction efficiency increased with time. Conclusion: Adipose derived stem cells can be transformed into acinar-like cells with secretory and α-amylase function, and their transformation efficiency increased with time. References | Related Articles | Metrics

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Effect of Osteopontin on Polarization of Macrophage in Vitro. FENG Li-hua, WANG Min, XIA Hai-bin, HUANG Kai-yao 2019, 35(5): 497-501.  DOI: 10.13701/j.cnki.kqyxyj.2019.05.020 Abstract ( 303 )   PDF (3531KB) ( 253 )   Objective: To study the effect of exogenous osteopontin (OPN) on the polarization of macrophage in vitro. Methods: Mouse RAW264.7 macrophages were cultured in vitro, and divided into groups as follows: (1) OPN dose-dependent experiment: the experimental group were treated with 0.1,0.5,1.0 mg/L OPN respectively in vitro; (2) M1 polarization experiment: set control group, LPS group, OPN group, LPS+OPN group; (3) M2 polarization experiment: there were control group, IL-4 group, OPN group, and IL-4+OPN group. After stimulated for 24 hours, quantitative real-time PCR (qRT-PCR) was used to measure mRNA levels of M1-type genes inducible nitric oxide synthase (iNOS), tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and M2-type genes CD206, Arginase-1 (Arg-1), and interleukin-10 (IL-10). Western blot was used to assay iNOS and Arg-1 protein. The expression of iNOS and CD206 was detected by immunofluorescence assay (IFA). Results: mRNA levels of M1 and M2-type genes both increased significantly in a dose-dependent manner on OPN group (P<0.05). The expression of M1-type genes was the highest on LPS+OPN group, while expression of M2-type genes was the highest on IL-4+OPN group (P<0.05). The Western blot and IFA results were consistent with qRT-PCR results. Conclusion: OPN may induce M1 or M2 polarization of macrophage, and has synergetic effect with LPS and IL-4, as a pleiotropic cytokine. References | Related Articles | Metrics

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Effect of Rosiglitazone on Osteoclastic Differentiation of RAW264.7 Cells under Estrogen. HU Ying-ying, HA Shan-shan, ZHU Ning-jing, SONG Ya-ling, WANG Chang-ning 2019, 35(5): 502-506.  DOI: 10.13701/j.cnki.kqyxyj.2019.05.021 Abstract ( 197 )   PDF (1344KB) ( 193 )   Objective: To investigate the effect and mechanism of rosiglitazone on osteoclastic differentiation of RAW264.7 cells under estrogen. Methods: The murine macrophage RAW264.7 cells lines were induced by RANKL in the presence or absence of rosiglitazone and/or estrogen for 5 days. The number of osteoclasts was detected by TRAP staining and the expression of osteoclast related genes and transcription factors were detected by qRT-PCR. The effects of rosiglitazone and/or estrogen on cell viability were detected by MTT. Results: Rosiglitazone and estrogen increased cell viability. RANKL induced osteoclast formation and the number of osteoclasts, osteoclast related genes and translation factors increased under rosiglitazone or estrogen stimulation (P<0.05). However, rosiglitazone inhibited osteoclast formation, and osteoclast related genes and translation factors were significantly decreased under estrogen (P<0.05). Conclusion: Rosiglitazone could inhibit osteoclast formation by TRAF6 and NFATc1 under estrogen. References | Related Articles | Metrics