Study on Transdifferentiation of Adipose-derived Stem Cells into Salivary Gland Acinar-like Cells.

doi:10.13701/j.cnki.kqyxyj.2019.05.019

Abstract

Abstract: Objective: To investigate whether adipose-derived stem cells (ADSCs) can be transformed into acinar-like cells with secretory function in the microenvironment simulated by submandubular gland cells (SMGCs). Methed: ADSCs were isolated from neonatal SD rats and cultured in vitro. Submandibular gland cell lysate was used to induce differentiation. To observe the morphological changes and expression of α-amylase antibody and cytokeratin-8 antibody, the content of amylase in the supernatant of cells induced at different periods was detected using amylase detection kit, and the conversion efficiency was analyzed. Results: The cells extracted from adipose tissue of rats had the ability to differentiate into multiple directions, which confirmed that they were adipose derived stem cells. After induced by submandibular gland cell lysate, α-amylase antibody and cytokeratin-8 antibody staining were positive, but negative in control group. This confirmed that the cells were successfully transformed into acinar-like cells, and the content of amylase in the supernatant increased with time, which indicated that the induction efficiency increased with time. Conclusion: Adipose derived stem cells can be transformed into acinar-like cells with secretory and α-amylase function, and their transformation efficiency increased with time.

Key words: Adipose stem cells, Submandibular gland cell lysate, Cell differentiation

YU Yang, XU Wen-pin, JIAO Kun, LIU Dong-rui, XING Ru-xiao, YE Jia-peng, ZHANG Wei. Study on Transdifferentiation of Adipose-derived Stem Cells into Salivary Gland Acinar-like Cells.[J]. Journal of Oral Science Research, 2019, 35(5): 493-496.

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