Effects of Progranulin Derived Engineered Protein Atsttrin on Osteogenic Differentiation of Periodontal Ligament Stem Cells

doi:10.13701/j.cnki.kqyxyj.2019.07.007

Abstract

Abstract: Objective: To explore the positive effect of Atsttrin on osteogenic differentiation of PDLSCs and the reversing effect of Atsttrin on TNF-α-inhibited PDLSCs osteogenic differentiation. Methods: PDLSCs were obtained and treated with 25 μg/L Atsttrin, 10 μg/L TNF-α, and 10 μg/L TNF-α + 25 μg/L Atsttrin, respectively. Alkaline phosphatase activity (ALP) and gene/protein expression levels of ALP and runt-related transcription factor-2 (Runx2) were assessed. The formation of mineralized nodules and determination of calcium content was observed. Results: Atsttrin enhanced the ALP activity and osteogenesis gene/protein expressions of PDLSCs, and promoted the formation of mineralized nodules. At three time points, the highest osteogenic differentiation and mineralization ability of PDLSCs were found in the 25 μg/L Atsttrin group. Conclusion: Atsttrin can directly promote the osteogenic differentiation and mineralization of PDLSCs, and can reverse the inhibitory effect of TNF-α on osteogenic differentiation of PDLSCs.

Key words: periodontal, ligament stem cells, cell differentiation , atsttrin

YU Miao, LI Ti, BAI Jianwen, WANG Limei, DUAN Xiaoqi, SUN Long, SUN Qinfeng. Effects of Progranulin Derived Engineered Protein Atsttrin on Osteogenic Differentiation of Periodontal Ligament Stem Cells[J]. Journal of Oral Science Research, 2019, 35(7): 643-646.

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