Effects of miR-146a Agonist on Expressions of Inflammatory Factors, IRAK1, and TRAK6 in Periodontal Tissues during Orthodontic Recurrence

doi:10.13701/j.cnki.kqyxyj.2020.05.016

Abstract

Abstract: Objective: To investigate the effect of miR-146a on the expression of inflammation-related genes and related signaling pathways in periodontal tissues of rats during orthodontic recurrence. Methods:24 male SD rats were divided into normal control group (group C) and orthodontic tooth movement model group. Devices of the model group were removed after 14 days, with randomly divided into model group (group N), model control group (with agomiR-control, group P), and model treatment group (with agomiR-146a, group T). Group N was sacrificed immediately after the devices were removed. Group T was injected with agomir-146a and group P was injected with the same amount of negative control for 7 days. Periodontal tissues of each group were collected after 7 days. The expression of miR-146a in tissue was detected by RT-PCR. The genes and proteins expressions of IRAK-1 and TRAF6 were detected by RT-PCR and Western blot. The expression of IL-6, IL-1β and TNF-α in periodontal tissue were detected by ELISA. The changes of periodontal tissue morphology were observed by HE staining. Results:Compared with group C, the expression level of miR-146a in group P was significantly decreased and the difference was significant (P<0.01). The expressions of IRAK-1, TRAF6, and inflammatory indicators were significantly increased (P<0.01). The infiltration of inflammatory cells was increased and the absorption of alveolar bone was obvious. After the injection of miR-146a agonist in group T, the expression of miR-146a was increased, and the expression of IRAK-1, TRAF6, and inflammatory indicators were significantly reduced (P<0.05). The infiltration of inflammatory cells was less, and the absorption of alveolar bone was mild. However, there were no significant changes in the expression of each index in group N (P>0.05). Conclusion:miR-146a agonist can reduce the expression of TRAF6 and IRAK1 to relieve periodontal inflammation caused by orthodontic recurrence.

Key words: orthodontic recurrence, miR-146a, periodontal tissue, inflammation

GAO Rui, YAN Weijun, AN Jingtao, SONG Bing, SHAO Ping. Effects of miR-146a Agonist on Expressions of Inflammatory Factors, IRAK1, and TRAK6 in Periodontal Tissues during Orthodontic Recurrence[J]. Journal of Oral Science Research, 2020, 36(5): 469-472.

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