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16 June 2020, Volume 36 Issue 5 Previous Issue    Next Issue

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A Review on Recent Update and Development in Implant Prosthesis and Its Complications ZHOU Yanmin, WANG Hanchi 2020, 36(5): 405-409.  DOI: 10.13701/j.cnki.kqyxyj.2020.05.001 Abstract ( 347 )   PDF (825KB) ( 434 )   The implant-supported prosthesis is a good substitute for traditional denture because of its good retention, comfort and recovery function. In addition to the traditional implant-supported overdentures and fixed implant-supported dentures, With the development of technology, zirconia material, cad-cam system, all-on-four concept have been applied in implant repair. Although implant technology has become a predictable and effective treatment, but also with clinical complications. Mastering the complication types of different repair designs can effectively prevent early complications, which is conducive to the therapeutic effect of implant repair. This article reviews the recent progress of prosthesis and complications in implant dentistry. References | Related Articles | Metrics

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Application and Biosafety of Graphene Materials in Stomatology WANG Nan, ZHOU Yanmin 2020, 36(5): 410-412.  DOI: 10.13701/j.cnki.kqyxyj.2020.05.002 Abstract ( 305 )   PDF (736KB) ( 386 )   Graphene materials have attracted much attention in the field of stomatology due to their superior properties. The research involves antimicrobial properties, osteogenic differentiation, tissue engineering scaffolds, drug delivery, and so on. However, some studies have shown that graphene and its derivatives may cause damage to cells and organs. Therefore, researchers begin to pay attention to the biosafety of graphene. In this paper, the application of graphene materials in stomatology is discussed, and the biosafety of graphene materials is reviewed. This paper may provide reference basis for the research and clinical application of related biological materials. References | Related Articles | Metrics

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Advancement of Optical Imaging on Precise Assessment for Surgical Margin of Oral Squamous Cell Carcinoma GAO Antian, LIN Zitong 2020, 36(5): 413-415.  DOI: 10.13701/j.cnki.kqyxyj.2020.05.003 Abstract ( 255 )   PDF (736KB) ( 238 )   Oral squamous cell carcinoma (OSCC) is the most common malignant tumors in the head and neck. The treatment of OSCC mainly focuses on surgical enlarged resection. As the tumors are located in the oral and maxillofacial region, enlarged resection will have a great impact on the appearance and function of patients, which can cause a significant decline in the quality of life of patients after surgery. On the other hand, insufficient resection can cause recurrence after surgery. Therefore, precise excision of lesions is of great significance for the survival and quality of life of patients with OSCC. In this paper, we reviewed the recent researches using optical imaging including fluorescence imaging, targeted fluorescence imaging, narrow band imaging, high-resolution microendoscopy, and raman spectroscopy on the precise assessment for surgical margin of OSCC. References | Related Articles | Metrics

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Research Progress on Smear Layer Removing Ability in Root Canals of Organic Acid Irrigation Solutions YUE Xin, SHEN Jing 2020, 36(5): 416-419.  DOI: 10.13701/j.cnki.kqyxyj.2020.05.004 Abstract ( 234 )   PDF (753KB) ( 294 )   A smear layer is produced during the instrumentation, which could be found both on the canal wall and into some dental tubules. Insufficient cleaning of the smear layer could result in failure of root canal therapy finally. Therefore, smear layer removing ability of root canal irrigation solutions had been researched by many scholars. Recently, one of the main root canal irrigation solutions for smear layer removal was organic acids and organic acid-based mixtures irrigation solutions. This article summarizes the current research progress on smear layer removing ability of organic acid irrigation solutions. It is suggested that the organic acid-based mixtures irrigation solutions, maleic acid, and tannic acid could be used the priority to remove the smear layer, which provides some reference for the clinic endodontic treatment. References | Related Articles | Metrics

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Development of Telemedicine and Its Application in Stomatology LU Bin, HE Fuming 2020, 36(5): 420-422.  DOI: 10.13701/j.cnki.kqyxyj.2020.05.005 Abstract ( 290 )   PDF (735KB) ( 358 )   In recent years, the state is working to improve the uneven distribution of medical resources and actively promote telemedicine. However, how to develop telemedicine in the field of stomatology has not been reported in China. Referring to the literature, the author finds that foreign countries have studied this field for many years, and formed the concept of teledentistry. This paper summarizes the literature of telemedicine and stomatology at home and abroad, and expounds the development of telemedicine and its application in stomatology. By introducing the experience of the development of teledentistry in foreign countries, the aim is to develop and perfect the new field of telestomatology in China. At the same time, this paper can help to strengthen the domestic stomatologist's awareness of telemedicine, and provide reference to carry out telemedicine. References | Related Articles | Metrics

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Identification of Hub Genes in Tongue Squamous Cell Carcinoma Based on Weighted Gene Co-expression Network Analysis LI Tianke, ZHANG Ying, ZHANG Suxin, CHEN Zhong, BAO Yang, ZHANG Guanhua 2020, 36(5): 423-427.  DOI: 10.13701/j.cnki.kqyxyj.2020.05.006 Abstract ( 229 )   PDF (3342KB) ( 346 )   Objective: To excavate hub genes closely related to tongue squamous cell carcinoma (TSCC). Methods: GSE34105 dataset was downloaded from Gene Expression Omnibus Database (GEO). Key modules were screened by weighted gene co-expression network analysis (WGCNA). Hub genes were screened by topological parameters of nodes in module networks, and survival analysis was used for external verification of hub genes. GO and KEGG enriched analysis and GSEA analysis were performed to obtain the biological processes involved in hub genes. Results: The turquoise module (Cor=0.88, P<0.05) which was most closely related to TSCC was obtained. Two hub genes, CAP1 and TMBIM6, were obtained based on Cytoscape, which were adverse prognostic factors. Their high expression was significantly associated with shortening the survival time of patients (P<0.05). GSEA analysis of their high expression group was mainly involved in cysteine and methionine metabolism, Vibrio cholerae infection, oxidative phosphorylation, DNA replication, basal excision repair, cell meiosis, protein production, fructose and mannose metabolism, Helicobacter pylori Epithelial signal transduction in infection. Conclusion: This study screened two hub genes by constructing a gene co-expression network, which may become a potential biomarker of TSCC gene, affecting the occurrence and prognosis of TSCC. References | Related Articles | Metrics

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Expression and Prognostic Significance of microRNAs in Peripheral Blood of Patients with Oral Squamous Cell Carcinoma YANG Kaicheng, YANG Lei, ZHAO Jianguang, LUO Fengyu, CHEN Yanping, CUI Zifeng, CHEN He, MAN Shasha 2020, 36(5): 428-432.  DOI: 10.13701/j.cnki.kqyxyj.2020.05.007 Abstract ( 231 )   PDF (832KB) ( 236 )   Objective: To screen differentially expressed microRNAs (miRNAs) in peripheral blood of patients with oral squamous cell carcinoma (OSCC) by qRT-PCR, and further to explore the relationship between differentially expressed miRNAs and clinical parameters and prognosis. Methods: The changes of miRNA in peripheral blood of patients with OSCC were observed by qRT-PCR, and the relationship between miRNA and clinicopathological parameters and prognosis was analyzed. Results: A total of 84 kinds of miRNAs were detected, of which 8 were up-regulated more than twice in peripheral blood of patients with OSCC and 18 were down-regulated less than 0.5 times. The expression of miR-222-3p and miR-423-5p in peripheral blood was significantly correlated with pTNM stage and lymph node metastasis (P＜0.05). The patients with high expression of miR-222-3p and low expression of miR-423-5p had a worse prognosis (P＜0.05). Multivariate analysis showed that the overexpression of miR-222-3p was an independent prognostic factor for the OSCC patients. Conclusions: miR-222-3p and miR-423-5p were abnormally expressed in the peripheral blood in OSCC patients, and they were expected to be biomarkers for early diagnosis and predicting prognosis of OSCC. References | Related Articles | Metrics

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Expression and Significance of EpCAM and β-catenin in Mucoepidermoid Carcinoma of Salivary Glands ZHU Yunna, ZHENG Huixia, XIAO Hong, WAN Huili, LV Jiale, LIANG Gang, LI Ning 2020, 36(5): 433-436.  DOI: 10.13701/j.cnki.kqyxyj.2020.05.008 Abstract ( 187 )   PDF (2270KB) ( 363 )   Objective: To investigate the expression and clinicopathologic significance of EpCAM and β-catenin in mucoepidermoid carcinoma (MEC). Methods: The expressions of EpCAM and β-catenin in 57 cases of MEC and 20 cases of normal salivary gland tissues were detected by immunohistochemical staining (Envision method). Results: The positive expression rate of EpCAM and β-catenin in MEC was significantly higher than that in normal tissues (P=0.002, P=0.001). The positive expression rates of EpCAM in MEC with high, medium, and low differentiation were 36.36%, 61.11%, and 82.35%, respectively (P=0.015), and the positive expression rates of β-catenin were 45.45%, 72.22%, and 88.24%, respectively, with statistically significant differences (P=0.016). Both EpCAM and β-catenin expression were correlated with patient age (P=0.004, P=0.004). EpCAM was correlated with lymph node metastasis (P=0.024), and the expression of EpCAM and β-catenin was unrelated to the gender of patients and the location of tumor. The expression of EpCAM and β-catenin in MEC was positively correlated (R=0.48, P < 0.001). Conclusion: Expressions of EpCAM and β-catenin were associated with MEC malignancies. Joint tests could help to evaluate MEC, assist in predicting prognosis, and provide basis for the targeted treatment of MEC. References | Related Articles | Metrics

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Correlation between Decorin and Oral Squamous Cell Carcinoma LAI Yongxian, NIE Minhai, CHEN Xiao, LIU Xuqian 2020, 36(5): 437-442.  DOI: 10.13701/j.cnki.kqyxyj.2020.05.009 Abstract ( 170 )   PDF (6529KB) ( 165 )   Objective: To investigate the expression and significance of Decorin (DCN) in oral squamous cell carcinoma (OSCC), and the possible mechanism by which DCN affects the development of OSCC. Methods: The expressions of the DCN, epidermal growth factor receptor (EGFR), oncogene C-Myc, and cyclin dependent kinase inhibitor (P21) in OSCC tissues and normal gingiva tissues were detected by immunohistochemistry. After being transfected with DCN plasmid, the expressions of EGFR, C-Myc, and P21 in HSC-3 cells were detected by Real time PCR and Western blot. Results: With the decreasing of differentiation degree of OSCC, the positive expressions of DCN and P21 proteins were decreased gradually, while the EGFR and C-Myc proteins were increased gradually (P<0.05). Compared with HK group and control group, the mRNA and protein levels of EGFR and C-Myc in transfection group were decreased, while the mRNA and protein level of P21 was increased (P<0.05). Conclusion: DCN is related to the occurrence and development of OSCC, which might reach the goal by regulating the expressions of EGFR, C-Myc, and P21. References | Related Articles | Metrics

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Differential Expression of Extracellular Matrix Protein DMP1-C and DMP1-N in Condylar Cartilage Before and After Occlusal Trauma Treatment LIU Peihong, MA Su, LI Yongheng, WANG Haixia 2020, 36(5): 443-448.  DOI: 10.13701/j.cnki.kqyxyj.2020.05.010 Abstract ( 180 )   PDF (6334KB) ( 122 )   Objective: To analyze the molecular mechanism of occlusal trauma on condylar mineralization in rats and to determine the clinical significance of occlusal adjustment through observing the histological changes of rats condylar cartilage and subchondral trabeculae and the differential expression of ECM protein DMP1-C and DMP1-N terminal in condylar cartilage. Methods: The rat models with 2 weeks occlusal trauma, 2 weeks after removal of 2 weeks occlusal trauma, and 4 weeks after removal of 2 weeks occlusal trauma. HE staining was used to observe the histological changes of condylar cartilage and subchondral trabeculae among the anterior, middle, and posterior of the condylar process, and immunohistochemistry was used to observe the differential expression of ECM protein DMP1-C and DMP1-N among the anterior, middle, and posterior of the condylar process before and after traumatic treatment. Results: Compared with the control group, the changes of condylar process in 2 weeks occlusal trauma group were significantly prominent in anterior area: mastocyte regions were widen or disappearing, calcified cartilage regions were significantly widen, condylar cartilage cells were in disorder; the expression of DMP1-C increased significantly, and the expression of DMP1-N decreased significantly. Subchondral trabeculae was in no orderly pattern. The rats condylar cartilage mastocyte regions and the calcified cartilage regions in 2 weeks treatment group were gradually recovered, and the arrangement of condylar cartilage cells become regular and gradually distributed along the stress direction; the expression of DMP1-C was lower than 2 weeks occlusal traumatism group but higher than normal control group. There were no significant difference between 4 weeks treatment group and control group in the thickness of condylar cartilage, the layers of cells, the distribution of cartilage cell and the expression of DMP1-C and DMP1-N. Conclusion: Occlusal trauma accelerated the process of endochondral ossification in rats condylar process, with the changes of the expression of ECM mineralization protein DMP1-C and DMP1-N, simulating the treatment after occlusal adjustment, the tissue of condylar process, subchondral bone mineralization, and the expression of DMP1 could back to normal. References | Related Articles | Metrics

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Long-term Prognosis and Influencing Factors of Autologous Tooth Transplantation after Root Development SUN Zhenlong, XUE Peng, TI Yunfei 2020, 36(5): 449-453.  DOI: 10.13701/j.cnki.kqyxyj.2020.05.011 Abstract ( 213 )   PDF (2530KB) ( 247 )   Objective: To investigate the long-term prognosis and influencing factors of autologous tooth transplantation after root development. Methods: 64 patients and 72 teeth with autologous tooth transplantation after root development were retrospectively chosen from January 2008 to January 2015 and the related clinical data and apical X-ray film were reviewed after operation. The influencing factors of clinical prognosis were analyzed according to age, sex, and root canal therapy experience. The 5-year cumulative survival rate and success rate of transplanted teeth were evaluated by Kaplan-Meier method and Log-Rank method. Results: The 5-year cumulative survival rate and cumulative success rate of 72 teeth with autologous transplanted teeth were respectively 83% and 65%. There was no significant difference in the cumulative survival rate and success rate between patients with age < 27 years old and ≥27 years old (P>0.05). There was no significant difference in the cumulative survival rate and success rate between patients gender (P>0.05). There was no significant difference in the cumulative survival rate between patients with or without root canal therapy (P>0.05). However, the success rate of patients with root canal therapy were significant higher than patients without root canal therapy (P<0.05). Conclusion: Autogenous tooth transplantation after root development possess good clinical prognosis and can be used as the important candidate for the restoration of missing teeth. Improving root canal therapy after operation is helpful to higher the long-term success rate of tooth transplantation. References | Related Articles | Metrics

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Finite Element Analysis on Upper Dental Arch Expansion using Invisible Appliance with Different Material Stiffness SU Tao, WANG Xingxing, XIANG Biao, WU Gang, ZOU Min 2020, 36(5): 454-458.  DOI: 10.13701/j.cnki.kqyxyj.2020.05.012 Abstract ( 285 )   PDF (1833KB) ( 286 )   Objective: To analyze the stress value, distribution characteristics of periodontal ligament, and the distance between the center of rotation and the root tip when using invisible appliance with different material stiffness to expand upper dental arch. Methods: Three-dimensional finite element models with elastic modulus at 415.6, 816.31, and 2400MPa were established. The upper premolars and molars were designed to buccally move 0.3mm to analyze the stress distribution. Results: Under experimental condition, the stress distribution tendency of group A, group B, and group C was similar. Specific performances: buccal cervical 1/3 was compressive stress, toward the apex gradually transiting to the tensile stress. The stress distribution on the lingual side was contrast. Specific performances: the lingual cervical 1/3 was tensile stress, toward the apex gradually transiting to the compressive stress. In three models, stress concentrated in the areas of apex, root bifurcation, and neck. The area of stress distribution was: Group A>Group C>Group B. In these groups, center of teeth rotation was located in the area of the junction of middle 1/3 and apex 1/3. Expanding upper dental arch, the elastic modulus of the appliance increased and the stress increased. Conclusion: The bigger the hardness of clear aligners, the bigger the stress in periodontal ligament and aligners. Upper premolars and molars tended to move buccally. References | Related Articles | Metrics

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Influencing Factors of Orthodontic Treatment on Root Resorption of Root Canal Therapy REN Yamin, WANG Yanan, CHEN Jinhong, GUO Renming, LIU Hanzhe, CUI Shuxia 2020, 36(5): 459-461.  DOI: 10.13701/j.cnki.kqyxyj.2020.05.013 Abstract ( 258 )   PDF (1827KB) ( 196 )   Objective: To study the effect of fixed orthodontic treatment on root resorption after root canal therapy and related factors. Methods: 96 patients with complete intraoral root canal treatment before orthodontic fixed treatment were selected. Cone beam computed tomography (CBCT) before and after orthodontic treatment was used to measure the length of root resorption after orthodontic treatment and to analyze the influence factors of orthodontic treatment on root resorption. Results: After orthodontic treatment, the root resorption of root canal therapy teeth was less than that of contralateral living pulp teeth of the same name (P<0.001). Gender was not a factor influencing root resorption in root canal therapy after orthodontic treatment (P＞0.05). Age was a factor influencing root resorption in root canal therapy after orthodontic treatment (P<0.05). The risk of root resorption in adults was greater than that in children. Dental position was a factor influencing root resorption in root canal therapy after orthodontic treatment (P<0.001). And the risk of root resorption in anterior teeth was greater than that in posterior teeth. Conclusion: The risk of root resorption in root canal therapy teeth is lower than that in contralateral living pulp teeth. Age and tooth position are the influencing factors of root resorption in root canal therapy teeth after fixed orthodontic treatment. References | Related Articles | Metrics

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Morphological Characteristics of Spee Curves of Different Ages in Class Ⅱ ZONG Lina, WANG Xiaoqin 2020, 36(5): 462-464.  DOI: 10.13701/j.cnki.kqyxyj.2020.05.014 Abstract ( 212 )   PDF (1116KB) ( 280 )   Objective: To explore the depth of Spee curve between Angle II patients with different ages. Methods: The three-dimensional digital model and lateral slices of the skull of 88 Class II patients who were admitted to the Department of Orthodontics, the First Hospital of Shanxi Medical University from July 2015 to July 2019 were collected. They were divided into two groups according to age: youth group (13 years old ≤ age ≤ 18 years old) and adult group (age > 18 years old). The components were grouped into a high angle group, a mean angle group, and a low angle group according to the GoGn-SN angle. The Spee curve depth of each group was measured and statistical analysis was performed. Results: There was a statistically significant difference in the depth of Spee curve between the Young's class II and the adult group (P＜0.05). There was a statistically significant difference in the depth of the Spee curve between the Class II and different vertical bone types (P＜0.05). Conclusion: The Spee curve depth of Angle II patients gradually decreases with age. References | Related Articles | Metrics

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Evaluation the Treatment Effect of Rapid Maxillary Expansion Combined with Maxillary Protraction of Class Ⅲ Malocclusion by GALL Line Bahaernisa·REZHAKE, LI Zhaoyang, HU Yichun, Dilifeire·TUERHONG, JIA Jun, Zhlihumaer·NUERAIHEMAITI, Gulibaha·MAIMAITILI 2020, 36(5): 465-468.  DOI: 10.13701/j.cnki.kqyxyj.2020.05.015 Abstract ( 238 )   PDF (2101KB) ( 183 )   Objective: To make an analysis on the appearance change after rapid maxillary expansion combined with maxillary protraction based on Andrews six elements of oralfacial harmony. Methods: A total of 25 patients with maxillary deficiency were collected. Before and after the treatments, cephalometric radiographs were measured using GAll line as a reference plane. The measuring results were compared with traditional evaluation index. Results: After treatment, all patients had obvious maxillary anterior displacement and mandibular posterior displacement. The differences of SNA, ANB, PP-FH, MP-FH, L1-MP, U1-SN, MP-FH, B-GALL, Pog-GALL, U1FA-GALL, U1-GALL, L1-GALL, LL-E, LL-GALL, and Ns-Sn-Pos before and after treatment were statistically significant. However, the differences of SNB, Wits, and Y axis were not statistically significant. Conclusion: The results of measurement indexes using GAll line as the reference plane were basically consistent with the results of traditional evaluation indexes. This evaluation method could effectively and intuitively reflect the improvement of the relationship among teeth, jaw, and face after the orthodontic treatment. References | Related Articles | Metrics

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Effects of miR-146a Agonist on Expressions of Inflammatory Factors, IRAK1, and TRAK6 in Periodontal Tissues during Orthodontic Recurrence GAO Rui, YAN Weijun, AN Jingtao, SONG Bing, SHAO Ping 2020, 36(5): 469-472.  DOI: 10.13701/j.cnki.kqyxyj.2020.05.016 Abstract ( 189 )   PDF (1716KB) ( 109 )   Objective: To investigate the effect of miR-146a on the expression of inflammation-related genes and related signaling pathways in periodontal tissues of rats during orthodontic recurrence. Methods:24 male SD rats were divided into normal control group (group C) and orthodontic tooth movement model group. Devices of the model group were removed after 14 days, with randomly divided into model group (group N), model control group (with agomiR-control, group P), and model treatment group (with agomiR-146a, group T). Group N was sacrificed immediately after the devices were removed. Group T was injected with agomir-146a and group P was injected with the same amount of negative control for 7 days. Periodontal tissues of each group were collected after 7 days. The expression of miR-146a in tissue was detected by RT-PCR. The genes and proteins expressions of IRAK-1 and TRAF6 were detected by RT-PCR and Western blot. The expression of IL-6, IL-1β and TNF-α in periodontal tissue were detected by ELISA. The changes of periodontal tissue morphology were observed by HE staining. Results:Compared with group C, the expression level of miR-146a in group P was significantly decreased and the difference was significant (P<0.01). The expressions of IRAK-1, TRAF6, and inflammatory indicators were significantly increased (P<0.01). The infiltration of inflammatory cells was increased and the absorption of alveolar bone was obvious. After the injection of miR-146a agonist in group T, the expression of miR-146a was increased, and the expression of IRAK-1, TRAF6, and inflammatory indicators were significantly reduced (P<0.05). The infiltration of inflammatory cells was less, and the absorption of alveolar bone was mild. However, there were no significant changes in the expression of each index in group N (P>0.05). Conclusion:miR-146a agonist can reduce the expression of TRAF6 and IRAK1 to relieve periodontal inflammation caused by orthodontic recurrence. References | Related Articles | Metrics

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Investigation and Analysis of Aggressive Periodontitis in Nanjing Area in Recent 6 Years ZHOU Wei, TANG Ya, LI Houxuan 2020, 36(5): 473-476.  DOI: 10.13701/j.cnki.kqyxyj.2020.05.017 Abstract ( 244 )   PDF (773KB) ( 158 )   Objective: To investigate the visits of patients with aggressive periodontitis in Nanjing. Methods: The electronic medical records and panoramic films of patients with aggressive periodontitis diagnosed in the Department of Periodontology, Nanjing Stomatological Hospital, Medical School of Nanjing University from January 1, 2013 to December 31, 2018 were collected through the electronic medical record system and PACS image analysis system. According to the medical records, the causes for visit, diagnostic rate, rate of tooth loss, treatment, and alveolar bone destruction of patients with aggressive periodontitis under 35 years old were statistically analyzed. Results: The main cause for visit of the patients with aggressive periodontitis was tooth loosening. The overall diagnostic rate within 6 years was 1.7%. Tooth loss accounted for 30.7% of the patients with aggressive periodontitis. 81.31% patients received periodontal treatment. The proportions of surgery and maintenance were 13.2% and 30.8%, respectively. Conclusion: Aggressive periodontitis in Nanjing presents a high incidence, a high rate of tooth loss, and great harm. However, the proportion of patients receiving systematic treatment is low. Therefore, popular science propaganda and education should be strengthened so as to raise patients’ awareness of prevention. References | Related Articles | Metrics

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Screening and Identification of microRNA on BSP Gene of Human Periodontal Ligament Cells Depressed by Lipopolysaccharide ZHANG Yuan, LI Xinyue 2020, 36(5): 477-480.  DOI: 10.13701/j.cnki.kqyxyj.2020.05.018 Abstract ( 176 )   PDF (1069KB) ( 140 )   Objective: To screen and identify miRNA which regulate gene expression of BSP and to analyze miRNA to regulate BSP gene expression during the osteogenic differentiation of hPDLCs depressed by LPS from P.g. Methods: Bioinformatics prediction and dual luciferase reporter gene analysis system were used to screen and verify the miR-129 that could be targeted to regulate BSP gene. miR-129 overexpression and expression inhibition vector plasmids were obtained to transfect hPDLCs. After treated each group of cells with 10mg/L P.g. LPS for 8 hours, the expression level of BSP was detected by qRT-PCR and western blotting. Results: Treatments with 10mg/L of P.g. LPS for 8 hours up-regulated the expression of miR-129, whereas BSP mRNA expression was induced. The expression levels of BSP in the experimental groups were lower than those in the mimic control group when miR-129 expression increased (P＜0.05). The protein expression levels of BSP in the experimental groups were higher than those in the inhibitor control group when miR-129 expression decreased(P＜0.05). Conclusion: In the process of P.g. LPS affecting osteogenic activity of hPDLCs, miR-129 may reduce its osteogenic activity by inhibiting BSP. References | Related Articles | Metrics

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Effect of Three-dimensional Pre-formed Titanium Mesh in Maxillary Anterior Region YUE Ke, DONG Heng, CHEN Li, YANG Guangwen, CHEN Lin, LIU Hui, ZHOU Na, MOU Yongbin 2020, 36(5): 481-485.  DOI: 10.13701/j.cnki.kqyxyj.2020.05.019 Abstract ( 237 )   PDF (2281KB) ( 435 )   Objective: To analyze the clinical effects of simultaneous implantation with three-dimensional pre-formed titanium mesh in the maxillary anterior region. Methods: Thirty-five patients who received delayed maxillary anterior teeth implantation from 2016 to 2018 were divided into two groups according to the types of bone grafting: three-dimensional pre-formed titanium mesh group and traditional GBR group, with a total of 45 implants. CBCT was used to measure the changes of alveolar bone volume in the bone grafting area at different stages. The gingival tissue esthetics and implant health index were also evaluated and analyzed. Results: Comparing the difference of alveolar bone absorption (L3) of two groups between bone grafting procedure (T1) and definitive restoration (T3), the three-dimensional titanium mesh group was less than (0.98±0.08 mm) L3 in the traditional GBR group (1.51±0.14 mm). The pink esthetic score (PES) of three-dimensional titanium mesh group (10.50±0.27) was higher than that of the traditional GBR group (9.45±0.19). The probing depth (PD), the gingival index (GI), and the plaque index (PLI) of the two groups in T3 showed no statistical difference. Conclusion: Three-dimensional pre-formed titanium mesh can provide more stable horizontal bone augmentation and better aesthetic effect for implant restoration in anterior maxilla. References | Related Articles | Metrics

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Clinical Efficacy and Bacteriological Analysis on Er:YAG Laser with Three Parameters in Treatment of Early Peri-implantitis JIA Yongna, GOU Xiaorui, JIANG Dandan, TANG Xiaoxue, ZHOU Zheng 2020, 36(5): 486-489.  DOI: 10.13701/j.cnki.kqyxyj.2020.05.020 Abstract ( 170 )   PDF (1685KB) ( 127 )   Objective: To evaluate the clinical efficacy and anaerobic bacteria content of Er:YAG laser irradiation with different energy parameters on early peri-implantitis. Methods: 90 patients with early peri-implantitis were included, who were randomly devided into four groups A, B, C, and D. Baseline values were recorded: plaque index (PLI), bleeding index (BI), probing depth (PD), and defect depth (DD). A, B and C groups were irradiated with Er:YAG laser at 40mj, 60mj, and 80mj, respectively. The degree of pain was recorded by visual analogue scale (VAS) during operation. Group D was treated with minocycline hydrochloride. Clinical treatment was 1 time per week for 3 times. The total clinical efficacy after the 8 weeks were compared. Gingival crevicular fluid for each tooth was collected to culture and analyze the content of anaerobic bacteria before treatment, after laser irradiation, and after scaling. Results: There was no significant difference in the rate of total clinical effective after 8 weeks for each group (P>0.05), but the change of bacterial content for each group was statistically significant (P<0.05). Conclusion: Among the safety range, the rate of total clinical effective for Er:YAG laser with different energy parameters on early peri-implantitis was not obvious after 8 weeks. However, the microbial changes were significant, and as the laser energy increasing, the bactericidal property was stronger. References | Related Articles | Metrics

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Effect of TRPM4 Channel on Proliferation and Migration of Human Buccal Mucosal Fibroblasts WANG Xueteng, NIE Minhai, ZUO Dongchuan, DENG Hao, ZENG Jin 2020, 36(5): 490-495.  DOI: 10.13701/j.cnki.kqyxyj.2020.05.021 Abstract ( 192 )   PDF (4794KB) ( 163 )   Objective: To verify the expression of TRPM4 in buccal mucosal fibroblasts and explore its effects on migration and proliferation of buccal fibroblasts. Methods: Human buccal mucosa fibroblasts were isolated and cultured in vitro, and the expression of TRPM4 in fibroblasts was evaluated by reverse transcription polymerase chain reaction (RT-PCR) and immunofluorescence. Whole cell patch clamp were performed to record the whole cell currents of TRPM4 channels. The effects of 9-phenanthrene or TRPM4 specific siRNA on the proliferation and migration of fibroblasts were estimated by MTT assay and cell scratch test, respectively. Results: TRPM4 was functional expressed in human buccal mucosa fibroblasts. Suppression the function of TRPM7 channel by 9-phenanthrene or specific siRNA inhibited the proliferation and migration capability of the cells. Conclusion: TRPM4 channels is involved in the regulation of proliferation and migration in human buccal mucosa fibroblasts. References | Related Articles | Metrics