Abstract: Objective: To compare the mRNA expression profiles of periodontal tissues surrounding tooth between normal rats and rats with orthodontic force, to screen different genes and perform GO analysis by microarray technology. Methods: Six SD rats were randomly selected and divided into experimental group and control group. The experimental group established rat orthodontic tooth movement model. According to the results of pre-experiment, the rats were sacrificed at 0h and 12h, respectively, and the maxillary first molar peripheral 1 mm periodontal tissues were harvested and sampled, then total RNA was extracted for rat whole genome microarray detection. GO analysis of differentially expressed genes was performed and RT-qPCR was used to confirm the results on core samples. Results: Microarray results showed that, in contrast to control group, a total of 463 differentially expressed genes, including 250 up-regulated genes and 213 down-regulated genes existed in the experimental group. Among the differentially expressed genes, there were 8 up-regulated genes involved in NF- kappa B signaling pathway. And the GO analysis involved 1189 different functional categories. Conclusion: Inflammation and neutrophil chemotaxis related genes up-regulated in periodontal tissue during orthodontics tooth movement.

Key words: Orthodontics tooth movement , Nuclear transcription factor-κB , Inflammation , Neutrophil , Chemotaxis