Effect of Caspase-3/8 on Apoptosis of Human Gingival Mesenchymal Stem Cells under Inflammation.

doi:10.13701/j.cnki.kqyxyj.2016.06.008

Abstract

Abstract: Objective: To explore the the effect of Caspase-3/8 on apoptosis of human gingival mesenchymal stem cells under inflammation. Methods: Gingival tissues with or without inflammation were obtained from the third impacted molars and gingival hyperplasia because of orthodontic treatment, respectively. Patients were all about 20-25 years old. Normal gingival mesenchymal stem cells (NGMSCs) and inflammatory gingival mesenchymal stem cells (IGMSCs) were isolated and cultured. Apoptosis rates of IGMSCs and NGMSCs were determined by V-FITC/PI Annexin double staining. Caspase-3 and Caspase-8 expression differences were detected by Real-Time PCR. Results: Apoptosis rate of IGMSCs (10.44%) was significantly more than that of NGMSCs (2.76%). Expressions of Caspase-3 and Caspase-8 in IGMSCs group were significantly higher than those of NGMSCs group (P<0.01) as well as TNF- α and IL-1β gene expressions. Conclusion: Caspase-3/8 plays an important role in regulating the proliferation and apoptosis of mesenchymal stem cells.

CLC Number:

R781.4

ZHANG Yang, LIU Jie, SHI Hai-gang, LI Xiao-guang, ZHANG Wei, LIU Na1, XU Lu-lu.. Effect of Caspase-3/8 on Apoptosis of Human Gingival Mesenchymal Stem Cells under Inflammation.[J]. Journal of Oral Science Research, 2016, 32(6): 584-587.

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