Effect of Primary Cilia on Expression of Osteogenic-related Genes of Human Periodontal Ligament Cells

doi:10.13701/j.cnki.kqyxyj.2018.01.003

Abstract

Abstract: Objective: To study the expression of osteogenic-related genes of human periodontal ligament cells (hPDLCs) by knocking down IFT88 to inhibit the formation of primary cilia. Methods: Control shRNA and shIFT88 were infected into hPDLCs. The expression of IFT88 was detected by qRT-PCR and Western blot. The formation of primary cilia was detected by immunofluorescence and the expressions of osteogenic-related genes COL1A1, OCN, Runx2, and BMP2 were detected by qRT-PCR. Results: The PKLO.1-shIFT88 was stably expressed in hPDLCs and inhibited the expression of IFT88 effectively (P<0.01). The formation of primary cilia was partly suppressed (P<0.01). The expressions of osteogenic-related genes like COL1A1, OCN, Runx2, and BMP2 were decreased significantly (P<0.01). Conclusion: Knockdown IFT88 inhibited the formation of primary cilia and reduced the expressions of osteogenic-related genes of hPDLCs.

Key words: Intraflagellar transport 88, Primary cilia, Human periodontal ligament cells, Osteogenic-related genes

CLC Number:

R781.4

JIANG Si-qi, YIN Feng-ying, ZHANG Fan, WANG Min, XIA Hai-bin. Effect of Primary Cilia on Expression of Osteogenic-related Genes of Human Periodontal Ligament Cells[J]. Journal of Oral Science Research, 2018, 34(1): 10-13.

References

[1] Iwata T, Washio K, Yoshida T, et al. Cell sheet engineering and its application for periodontal regeneration [J]. J Tissue Eng Regen Med, 2015, 9(4)∶343-356
[2] Garlet TP, Coelho U, Silva JS, et al. Cytokine expression pattern in compression and tension sides of the periodontal ligament during orthodontic tooth movement in humans [J]. Eur J Oral Sci, 2007, 115(5)∶355-362
[3] Wescott DC, Pinkerton MN, Gaffey BJ, et al. Osteogenic gene expression by human periodontal ligament cells under cyclic tension [J]. J Dent Res, 2007, 86(12)∶1212-1216
[4] Wang T, Wang P, Cao Z, et al. Effects of BMP9 and pulsed electromagnetic fields on the proliferation and osteogenic differentiation of human periodontal ligament stem cells [J]. Bioelectromagnetics, 2017, 38(1)∶63-77
[5] 曹木青,潘俊敏.纤毛及纤毛相关疾病研究进展[J].中国细胞生物学学报,2012,34(9)∶849-856
[6] Yuan X, Serra R A, Yang S. Function and regulation of primary cilia and intraflagellar transport proteins in the skeleton [J]. Ann N Y Acad Sci, 2015, 1335∶78-99
[7] Serra R. Role of intraflagellar transport and primary cilia in skeletal development [J]. Anat Rec (Hoboken), 2008, 291(9)∶1049-1061
[8] Bodle JC, Rubenstein CD, Phillips M E, et al. Primary cilia: the chemical antenna regulating human adipose-derived stem cell osteogenesis [J]. PLoS One, 2013, 8(5)∶e62554
[9] Kinumatsu T, Shibukawa Y, Yasuda T, et al. TMJ development and growth require primary cilia function [J]. J Dent Res, 2011, 90(8)∶988-994
[10] Qiu N, Xiao Z, Cao L, et al. Disruption of Kif3a in osteoblasts results in defective bone formation and osteopenia [J]. J Cell Sci, 2012, 125(Pt 8)∶1945-1957
[11] 尹凤英,孙慧芳,王敏,等. 人牙周膜细胞和牙周膜组织的初级纤毛观察[J].口腔医学研究, 2016, 32(2)∶131-133
[12] Hampl M, Cela P, Szabo-Rogers H L, et al. Role of Primary Cilia in Odontogenesis [J]. J Dent Res, 2017∶22034517713688
[13] Yuan X, Yang S. Deletion of IFT80 Impairs Epiphyseal and Articular Cartilage Formation Due to Disruption of Chondrocyte Differentiation [J]. PLoS One, 2015, 10(6)∶e0130618
[14] Ohazama A, Haycraft C J, Seppala M, et al. Primary cilia regulate Shh activity in the control of molar tooth number [J]. Development, 2009, 136(6)∶897-903