Effect of Tea Polyphenols on ICAM-1 Expression in LPS-mediated Human Periodontal Ligament Fibroblast Cells.

doi:10.13701/j.cnki.kqyxyj.2018.10.014

Abstract

Abstract: Objective: To investigate the effect of tea polyphenols (TP) on ICAM-1 expression in LPS-treated human periodontal ligament fibroblast cells (HPDLFs). Methods: HPDLFs were obtained from periodontal tissues by modified-cultured method. After induced by LPS, the control group was only treated with LPS, the experiment groups were treated with 100 μg/mL TP (TP100 group) or 200 μg/mL TP (TP200 group). The levels of ICAM-1 secretion in three groups were tested by ELISA. And the mRNA expression of ICAM-1 was detected by real-time PCR. Results: ICAM-1 was inhibited by TP after induced by LPS, and its activity was decreased significantly (P<0.05). The ICAM-1 mRNA levels of TP treated groups were significantly lower than that of LPS group (P<0.05). Conclusion: TP inhibited the expression of ICAM-1 and the effect was more significantly at 100 μg/mL. This result suggested that the anti-inflammatory mechanism of TP might relate to the inhibition of ICAM-1.

Key words: Tea polyphenols, Lipopolysaccharide, Human periodontal ligament cells, Intercellular adhesion molecule-1

FAN Qin, GUAN Xiao-yan, LI Xiao-na, LIU Jian-guo.. Effect of Tea Polyphenols on ICAM-1 Expression in LPS-mediated Human Periodontal Ligament Fibroblast Cells.[J]. Journal of Oral Science Research, 2018, 34(10): 1089-1092.

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