Effect of TRAF6 Silencing by siRNA on Proliferation of Human Periodontal Ligament Cells Treated with LPS.

doi:10.13701/j.cnki.kqyxyj.2017.04.003

Abstract

Abstract: Objective: To investigate the effect of siRNAtargeting TRAF6 on proliferation of human periodontal ligament cells(hPDLC) under LPS challenge. Methods: hPDLC was isolated from human periodontal ligament. The immunohistochemistry was used to examinethe expression of cytokeratin and vimentin. Lipofectamine was used to transfect TRAF6 siRNA and control siRNA into hPDLC, and then the cells were stimulated by LPS. RT-PCR was employed to determine TRAF6 mRNA. CCK-8 was used to test the proliferation of hPDLC under LPS challenge. Results: Immunohistochemistry confirmed that the cells were hPDLC. Compared with the control group, the mRNA expression of TRAF6 in TRAF6 siRNA group was decreased. The A value of TRAF6 knockdown cells was significantly decreased compared with thecontrol group(P<0.001).The proliferation of TRAF6 siRNA group treated with 10μg /mL LPS was also significantly decreased compared with other groups(P<0.001). Conclusion: The results suggest that TRAF6 knockdown inhibits the proliferation upon LPS challenge, which indicates thatTRAF6 maybe a target for periodontitis therapy.

Key words: TRAF6 , siRNA , Lipopolysaccharides, Cell proliferation

CLC Number:

R781.4

CHEN Qing-yong, LI Xia, ZHANG Fang,WANG Jin-hua.. Effect of TRAF6 Silencing by siRNA on Proliferation of Human Periodontal Ligament Cells Treated with LPS.[J]. Journal of Oral Science Research, 2017, 33(4): 358-361.

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