Estrogen and Progesterone Modulates Proliferation and Differentiation of Human Periodontal Ligament Cells

doi:10.13701/j.cnki.kqyxyj.2019.07.010

Abstract

Abstract: Objective: To detect the proliferation and the expression of related genes of osteogenic differentiation in human periodontal ligament cells (hPDLCs) by estrogen and progesterone. Methods: The hPDLCs were isolated, cultured and stimulated with estrogen and/or progesterone for 24h or 72h. MTT assay was performed to assess the effects of estrogen and/or progesterone on cell proliferation. To quantify the effects of estrogen and/or progesterone on osteogenic differentiation of hPDLCs, real-time PCR was performed in estrogen and/or progesterone-treated hPDLCs. Results: Estrogen and/or progesterone could stimulate the proliferation of the hPDLCs, and promote the expression of PLAP-1, OPG, MGP, ODF, COL-1, and MMP-9 in hPDLCs. Furthermore, estrogen and/or progesterone exerted the synergistic effect in the promotion of cell proliferation and differentiation of hPDLCs. The effect of estrogen or progesterone treatment on osteoblast differentiation of mineralized hPDLCs cells was increased in contrast to the control group (P<0.05). The combined treatment group was significantly higher than the single treatment group (P<0.01). Conclusion: Estrogen and progesterone can synergistically stimulate the proliferation and differentiation of the hPDLCs.

Key words: estrogen , progesterone, human periodontal ligament cells, cell proliferation , differentiation

CHENG Haiyan, SHEN Lanhua, ZHANG Rui, MENG Lingna, LIU Di, XING Beiyu, TAO Guannan. Estrogen and Progesterone Modulates Proliferation and Differentiation of Human Periodontal Ligament Cells[J]. Journal of Oral Science Research, 2019, 35(7): 657-660.

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