Journal of Oral Science Research ›› 2020, Vol. 36 ›› Issue (6): 581-584.DOI: 10.13701/j.cnki.kqyxyj.2020.06.018

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Effects of Polishing Methods on Surface Morphology of and Cell Proliferation of Human Gingival Fibroblasts on Two Kinds of Filling Materials

SHANG Jiaxin1, SU Rui2, MA Chong3, LI Xinyue2*   

  1. 1. Medical College, NanKai University, Tianjin 300071, China;
    2. Tianjin Stomatological Hospital, Tianjin 300041, China;
    3. Taikangbaibo Stomatological Hospital, Tianjin 300000, China
  • Received:2019-10-08 Online:2020-07-03 Published:2020-07-06

Abstract: Objective: To observe the surface morphology and biocompatibility of the two polished materials. Methods: The specimens were prepared by using resin-modified glass ionomer GC Fuji Ⅱ LC and nano-composite resin 3M FiltekTM Z350 XT. Silicon particles and super-snap polishing system were used for polishing. The surface morphology of the materials and the root slices were observed by SEM. Human gingival fibroblasts were inoculated on the specimens, and cell proliferation test was conducted by MTT method on the 1st, 3rd, 5th and 7th day. Results: Under 5000 times electron microscope, the surface of resin was smoother than that of glass ionomer, and the surface of resin combined polishing system or silicon particle was smoother than that of glass ion combined polishing system or silicon particle. In the cell proliferation test, the 5d resin combined with super-snap had higher absorbance values than both glassy ion combined with silicon particles and super-snap, and the resin combined with silicon particles had higher absorbance values than glassy ion combined with silicon particles (P<0.05). Conclusion: 3M FiltekTM Z350 XT resin combined with super-snap polishing system can obtain a smoother surface, and the super-snap polishing system can obtain better cell compatibility than the silicon particle polishing treatment.

Key words: composite resin, glass ionomer, human gingival fibroblasts, scanning electron microscope, polishing, cell proliferation