Effects of Collagen Gel Encapsulating Naringin on Proliferation of Human Dental Pulp Stem Cells.

doi:10.13701/j.cnki.kqyxyj.2019.06.008

Abstract

Abstract: Objective: Naringin/collagen gel complex was constructed and its effects on the proliferation of human dental pulp stem cells (hDPSCs) were investigated. Methods: HDPSCs were isolated and cultured in vitro. Proliferation ability of hDPSCs in various concentrations of collagen gel was analyzed using a cell counting kit -8 (CCK-8) assays. HDPSCs and different concentrations of naringin were encapsulated in the collagen gel. CCK-8 assay was used to evaluate the effect of naringin/collagen gel complex on the proliferation of hDPSCs. Results: The optimal collagen gel concentration was 1 g/L, which could enhance the adherence and growth of hDPSCs. In addition, the best ratio of naringin to collagen gel was 0.2∶1, which promoted the growth and proliferation of hDPSCs. Conclision: Naringin/collagen gel complex possesses a good cellular compatibility with hDPSCs. As a scaffold material, it can provide space for hDPSCs to proliferate.

Key words: Human dental pulp stem cells, Naringin, Collagen gel, Cell proliferation

XIAO Wan-lu, PAN Shuang, HOU Ting-ting, LI Yan-ping, HE Li-na, NIU Yu-mei. Effects of Collagen Gel Encapsulating Naringin on Proliferation of Human Dental Pulp Stem Cells.[J]. Journal of Oral Science Research, 2019, 35(6): 541-545.

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