Effects of Platelet-rich Fibrin Extract on Periodontal Ligament Cells

doi:10.13701/j.cnki.kqyxyj.2020.01.011

Abstract

Abstract: Objective: To investigate the effect of platelet-rich fibrin extract (PRF) on the proliferation, migration, and osteogenesis of human periodontal ligament cells (hPDLCs). Methods: The hPDLCs were primarily cultured. After identification, the effect of PRF on the proliferation of hPDLCs was detected by MTT. The cells was divided into control group and PRF group (concentration 50%). The ALP activity of hPDLCs cultured for 7d and 14d was detected by alkaline phosphatase assay. The migration of hPDLCs was detected by transwell. The mineralization function of cells was observed by alizarin red staining. The expression of BMP2 and Runx2 was detected by western blotting. Results: MTT results showed that OD value of each group increased gradually with time, and the fastest increase was in PFR2 group. Compared with the control group, ALP activity in PRF group increased significantly on day 7 and 14 (P<0.05). Transwell results showed that the number of migrating cells in PFR group was significantly higher than that in control group (P<0.05). Alizarin red staining showed that the optical density of mineralized nodules in PRF group was higher than that in control group at all time points (P<0.05). Western blotting results showed that the expressions of Runx2 and BMP2 in PFR group were higher than those in control group (P<0.05). Conclusion: PRF can promote the proliferation, migration, and osteogenic differentiation of hPDLCs, and its mechanism of promoting bone formation needs further study

Key words: platelet-rich fibrin, cell proliferation, osteogenesis, human periodontal ligament cells

HUANG Yizhi, GUO Jun, YANG Jian. Effects of Platelet-rich Fibrin Extract on Periodontal Ligament Cells[J]. Journal of Oral Science Research, 2020, 36(1): 41-45.

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