Effects of Estrogen and Progesterone Sustained Release Gel on Alveolar Bone Regeneration of Ovariectomized Periodontitis Rats

doi:10.13701/j.cnki.kqyxyj.2020.01.010

Abstract

Abstract: Objective: To study the effect of estrogen-progesterone sustained-release gel on the regeneration of alveolar bone in OVX periodontitis rats. Methods: Female rats aged 6 months were randomly divided into two groups: the ovariectomized group (extracted rat bilateral ovaries) and the sham operation group (bilateral ovarian adipose tissue was removed by the same method). Serum calcium, phosphorus, alkaline phosphatase, and E2 concentrations were detected 12 weeks after ovariectomized. After 12 weeks, a 0.2 mm ligature was ligated to the unilateral first molar. After ligation for 4 weeks, a rat model of periodontitis was established and bone loss was observed by Micro-CT. After removing the ligation, the rats were divided into sham saline group, sham treatment group, OVX saline group, and OVX treatment group. Estrogen-progesterone sustained-release gel and saline were injected into the periodontal pocket of rats, respectively, and Micro-CT analysis and HE histological staining were performed after 8 weeks of treatment. Results: After ovariectomized, the bone mineral density and calcium, phosphorus, and E2 concentrations of serum were reduced, however, ALP activity was increased. After periodontal ligation, alveolar bone was absorbed in horizontal direction in sham group. However, alveolar bone was absorbed not only in horizontal but also in vertical direction in OVX group, and the root bifurcation was exposed. After application of estrogen and progesterone sustained-release gel, alveolar bone density (BMD) was increased, trabecular bone number (Tb.N) was increased, trabecular bone separation degree (TbTh) was increased, trabecular bone connection number (Tb.P) was increased, and alveolar bone formation was increased. Conclusion: Estrogen and progesterone sustained-release gel promotes the alveolar bone regeneration of OVX periodontitis rats.

Key words: OVX, periodontitis, estrogen and progesterone, sustained release gel

YUAN Gongjie, DU Xinjie, SHEN Lanhua. Effects of Estrogen and Progesterone Sustained Release Gel on Alveolar Bone Regeneration of Ovariectomized Periodontitis Rats[J]. Journal of Oral Science Research, 2020, 36(1): 37-40.

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