Quantitative Analysis of Cranial Suture Distraction Osteogenesis in Mice

doi:10.13701/j.cnki.kqyxyj.2020.08.012

Abstract

Abstract: Objective: To observe cranial suture distraction osteogenesis process quantitatively and to lay the foundation for further research. Methods: 30 healthy C57BL/6J mice aged 4-5 weeks were randomly divided into sham operation group and expansion group (n=15 per group). Mice in each group were sacrificed at 7, 14, and 28 days after stretch. Newly formed bone was analyzed by Micro CT scan and histological staining. Results: Sagittal sutures in expansion group were enlarged by (3.55±0.53) mm averagely(P<0.05). Micro CT and histological analysis showed that there was new bone formation in the distraction area, which sited mostly at edge of parietal bones. Conclusion: The cranial suture distraction osteogenesis could be directly restored. Bone parameters could be comprehensively and quantitatively analyzed using Micro CT, which has a broad application prospect for the study of suture distraction osteogenesis.

Key words: cranial suture, distraction osteogenesis, quantitative analysis

WANG Jialu, ZHANG Weibing. Quantitative Analysis of Cranial Suture Distraction Osteogenesis in Mice[J]. Journal of Oral Science Research, 2020, 36(8): 753-756.

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