Effects of N-butanol Extract of Cucumissativus Seeds on Proliferation and Odontogenic Differentiation of Human Dental Pulp Stem Cells.

doi:10.13701/j.cnki.kqyxyj.2018.04.011

Abstract

Abstract: Objective: To investigate the effects of n-butanol extract of Cucumis sativus seeds (CSB) on proliferation and odontogenic differentiation of human dental pulp stem cells (hDPSCs). Methods: HDPSCs were isolated and exposed to different concentrations of CSB solutions. The proliferation ability was evaluated by Cell Counting Kit-8 (CCK-8) assay. The odontogentic potential of hDPSCs was assessed by alkaline phosphatase (ALP) staining and activity assay. In addition, Western blot analysis was conducted to identify the expressions of dentin sialophosphoprotein (DSPP) and Collagen I (COL I) which were related proteins on odontogenic differentiation of hDPSCs. Results: CCK-8 assay showed that CSB significantly enhanced the proliferation of hDPSCs at a concentration of 100 mg/L, while CSB remarkably suppressed the growth of hDPSCs at a higher concentration of 500 mg/L. The hDPSCs cultured with 100 mg/L CSB obviously induced positive ALP staining and had higher ALP activity compared with the control group. Furthermore, Western blot analysis demonstrated that the expression levels of odontogenic proteins DSPP and COL I were significantly up-regulated in 100 mg/L CSB-treated hDPSCs compared with the control group. Conclusion: The results indicate that an appropriate concentration of CSB could enhance the proliferation and odontogenic differentiation of hDPSCs.

Key words: N-butanol extract of cucumis sativus seeds, Human dental pulp stem cells, Proliferation, Odontogenic differentiation

CHE Jing-yi, LI Yan-ping, PAN Shuang, HE Li-na, NIU Yu-mei. Effects of N-butanol Extract of Cucumissativus Seeds on Proliferation and Odontogenic Differentiation of Human Dental Pulp Stem Cells.[J]. Journal of Oral Science Research, 2018, 34(4): 380-383.

References

[1] 田桂丽,苗晗,张圣平,等.黄瓜籽化学成分及其功效[J].中国蔬菜,2014,8(1)∶7-11
[2] 裴启洋,佟鑫,贾天柱.黄瓜子不同提取部位对体外培养大鼠成骨细胞的影响[J].中成药,2014,36(4)∶680-684
[3] 于天瑶,潘爽,何丽娜,等.Rho激酶抑制剂Y-27632对人牙髓干细胞增殖能力的影响[J].口腔医学研究,2016,32(3)∶278-281
[4] Collart-Dutilleul PY, Secret E, Panayotov I, et al. Adhesionand proliferation of human mesenchymal stem cells fromdental pulp on porous silicon scaffolds [J]. ACS Appl Mater Interfaces, 2014, 6(3)∶1719-1728
[5] Gronthos S, Brahim J, Li W, et al. Stem cell properties of human dental pulp stem cells [J]. J Dent Res, 2002, 81(8)∶531-535
[6] Al Akeel R, Al-Sheikh Y, Mateen A, et al.Evaluation of antibacterial activity of crude protein extracts from seeds of six different medical plants against standard bacterial strains [J]. Saudi J Biol Sci, 2014, 21(2)∶147-151
[7] Kim JG, Son KM, Park HC, et al.Stimulating effects of quercetin and phenamil on differentiation of human dental pulp cells [J]. Eur J Oral Sci, 2013, 121(6)∶559-565
[8] Wang X, Gao A, Chen Y, et al.Preparation of cucumber seed peptide-calcium chelate by liquid state fermentation and its characterization [J]. Food Chem, 2017, 229∶487-494
[9] 蔡伟明,李延平.黄瓜籽多肽的抗骨质疏松作用[J].中国医院药学杂志,2011,31(16)∶1333-1335
[10] Yoshida S, Wada N, Hasegawa D, et al. Semaphorin 3A induces odontoblastic phenotype in dental pulp stem cells [J]. J Dent Res, 2016, 95(11)∶1282-1290
[11] Suzuki S, Haruyama N, Nishimura F, et al. Dentin sialophosphoprotein and dentin matrix protein-1: two highly phosphorylated proteins in mineralized tissues [J]. Arch Oral Biol,2012,57(9)∶1165-1175
[12] Guo W, He Y, Zhang X, et al. The use of dentin matrix scaffold and dental follicle cells fordentin regeneration [J]. Biomaterials, 2009, 30(35)∶6708-6723

[1]	GUAN Mengying, HE Lina, PAN Shuang, LI Yanping, LIU Huimei, NIU Yumei. Effects of Canonical Wnt Signaling Pathway on Proliferation, Migration, and Odontogenic Differentiation of hDPSCs [J]. Journal of Oral Science Research, 2020, 36(9): 861-865.
[2]	QIU Yinfeng, TANG Ying, SHEN Yifen, LIU Chao, SHEN Hao, GU Yongchun, YU Jinhua. Effects of Fluoride Exposure on Cell Proliferation and Osteogenic Differentiation of Periodontal Ligament Stem Cells [J]. Journal of Oral Science Research, 2020, 36(9): 866-870.
[3]	MA Lan, YU Yijun, LIU Hanxiao, LIU Chao, MIAO Leiying. Berberine Stimulates Osteogenesis/Odontogenic Differentiation of Human Dental Pulp Stem Cells [J]. Journal of Oral Science Research, 2020, 36(7): 639-643.
[4]	CHEN Zhenyu, PAN Yingjing, ZHANG Yan, HE Xi, HUANG Dahong. Effects of Puerarin on Biological Properties of MC3T3-E1 under High Concentration of Glucocorticoid Circumstance [J]. Journal of Oral Science Research, 2020, 36(6): 544-547.
[5]	SHANG Jiaxin, SU Rui, MA Chong, LI Xinyue. Effects of Polishing Methods on Surface Morphology of and Cell Proliferation of Human Gingival Fibroblasts on Two Kinds of Filling Materials [J]. Journal of Oral Science Research, 2020, 36(6): 581-584.
[6]	WANG Xueteng, NIE Minhai, ZUO Dongchuan, DENG Hao, ZENG Jin. Effect of TRPM4 Channel on Proliferation and Migration of Human Buccal Mucosal Fibroblasts [J]. Journal of Oral Science Research, 2020, 36(5): 490-495.
[7]	YIN Xiaowei, ZHANG Shuang, DENG Haotian, HE Lina, LI Yanping, ZHANG Weiwei, PAN Shuang, NIU Yumei. Effect of Integrin Alpha 6 on Proliferation and Odontoblast Differentiation of Human Dental Pulp Stem Cells [J]. Journal of Oral Science Research, 2020, 36(12): 1132-1136.
[8]	ZHANG Weijia, PAN Shuang, LI Yanping, HE Lina, WU Zuodong, NIU Yumei. Effect of In Vitro Indirect Co-culture of Rabbit Dental Pulp Stem Cells with Schwann Cells on Proliferation and Neuronal Differentiation of Rabbit Dental Pulp Stem Cells [J]. Journal of Oral Science Research, 2020, 36(10): 968-972.
[9]	YAN Na, HUANG Tao, ZHANG Zhongyue, ZHANG Shuhua, WANG Lihua, HAN Guoliang. Functions of Platelet-rich Fibrin on Proliferation and Osteogenic Differentiation of Dental Pulp Stem Cells [J]. Journal of Oral Science Research, 2020, 36(10): 973-977.
[10]	HUANG Yizhi, GUO Jun, YANG Jian. Effects of Platelet-rich Fibrin Extract on Periodontal Ligament Cells [J]. Journal of Oral Science Research, 2020, 36(1): 41-45.
[11]	TANG Xiaoyue, ZHU Hao, CAO Can. Effects of Resveratrol on Proliferation and Differentiation of Osteoblasts Induced by High Glucose through SIRT1/FOXO1 Pathway [J]. Journal of Oral Science Research, 2020, 36(1): 79-84.
[12]	XIE Long, SANG Lei, SONG Zhuoying, JIANG Yanjun. Diagnostic Values of Salivary MicroRNA-375 for OSCC and Its Effects on Biological Function of OSCC Cells [J]. Journal of Oral Science Research, 2019, 35(9): 883-888.
[13]	HOU Tingting, PAN Shuan, LI Yanping, HE Lina, XIAO Wanlu, NIU Yumei. Naringin in Nanometer Hydroxyapatite Collagen Scaffold Enhanced the Proliferation and Osteoblastic Differentiation of Human Dental Pulp Stem Cells in Vivo [J]. Journal of Oral Science Research, 2019, 35(8): 744-747.
[14]	CHENG Haiyan, SHEN Lanhua, ZHANG Rui, MENG Lingna, LIU Di, XING Beiyu, TAO Guannan. Estrogen and Progesterone Modulates Proliferation and Differentiation of Human Periodontal Ligament Cells [J]. Journal of Oral Science Research, 2019, 35(7): 657-660.
[15]	SHAN Qiusheng, LI Guolin, XU Jiaqi. TH287 Inhibits Proliferation and Metastasis of CAL27 [J]. Journal of Oral Science Research, 2019, 35(7): 704-707.