Gene Expression of Human Dental Pulp Stem Cells during Osteogenic Differentiation.

doi:10.13701/j.cnki.kqyxyj.2018.04.010

Abstract

Abstract: Objective: To evaluate the differences in gene expression of human dental pulp stem cells (hDPSCs) after osteogenetic induction.. Methods: hDPSCs were cultured by explant method and the capability of multi-differentiation in hDPSCs was identified. The gene expressions were evaluated by microarray assay and quantitative real-time PCR after osteogenetic induction. Results: hDPSCs could differentiate into osteoblasts and adipocytes. The microarray assay revealed that the expression of 1284 genes were upregulated or downregulated more than twice after osteogenetic induction. Of these, the transcription of osteomodulin (OMD) gene was upregulated by fifty times. The results of qPCR indicated that the expression of OMD was gradually increased during osteogenetic induction. Conclusion: The osteogenetic differentiation of hDPSCs is regulated by multiple genes. OMD is one of these genes, and its expression is time-dependent and coincides with osteogenesis.

Key words: Human dental pulp stem cells, Osteogenetic differentiation, Osteomodulin

LIN Wen-zhen, GAO Li, NIU Chen-guang, HU Xu-chen, YUAN Ke-yong, MA Rui, HUANG Zheng-wei. Gene Expression of Human Dental Pulp Stem Cells during Osteogenic Differentiation.[J]. Journal of Oral Science Research, 2018, 34(4): 375-379.

References

[1] Diomede F, Rajan TS, Gatta V, et al. Stemness maintenance properties in human oral stem cells after long-term passage [J]. Stem Cells Int, 2017, 2017∶5651287
[2] Gronthos S, Mankani M, Brahim J, et al. Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo [J]. Proc Natl Acad Sci U S A, 2000, 97(25)∶13625-13630
[3] Xia XL, Brophy S, Zhou SM. Learning differentially expressed gene pairs in microarray data [J]. Stud Health Technol Inform, 2017, 235∶191-195
[4] Nakamura S, Yamada Y, Katagiri W, et al. Stem cell proliferation pathways comparison between human exfoliated deciduous teeth and dental pulp stem cells by gene expression profile from promising dental pulp [J]. J Endod, 2009, 35(11)∶1536-1542
[5] 高丽,江文欣,牛晨光,等.干细胞表面标志物在牙髓干细胞中的表达[J].上海口腔医学,2015,24(3)∶257-262
[6] Struys T, Moreels M, Martens W, et al. Ultrastructural and immunocytochemical analysis of multilineage differentiated human dental pulp- and umbilical cord-derived mesenchymal stem cells [J]. Cells Tissues Organs, 2011, 193(6)∶366-378
[7] 刘金凤,牛雪微,超博,等.牙髓再生的研究现状[J].口腔医学研究, 2017, 33(1)∶108-111
[8] Augello A, De Bari C. The regulation of differentiation in mesenchymal stem cells [J]. Hum Gene Ther, 2010, 21(10)∶1226-2238
[9] Liu J, Jin T, Chang S, et al. Matrix and TGF-beta-related gene expression during human dental pulp stem cell (DPSC) mineralization [J]. In Vitro Cell Dev Biol Anim, 2007, 43(3/4)∶120-128
[10] Datta P, Ghosh P, Ghosh K, et al .in vitro ALP and osteocalcin gene expression analysis and in vivo biocompatibility of N-methylene phosphonic chitosan nanofibers for bone regeneration [J]. J Biomed Nanotechnol, 2013, 9(5)∶870-879
[11] Sommarin Y, Wendel M, Shen Z, et al. Osteoadherin, a cell-binding keratan sulfate proteoglycan in bone, belongs to the family of leucine-rich repeat proteins of the extracellular matrix [J]. J Biol Chem, 1998, 273 (27)∶16723- 16729
[12] Ninomiya K, Miyamoto T, Imai J, et al. Osteoclastic activity induces osteomodulin expression in osteoblasts [J]. Biochem Biophys Res Commun, 2007, 362(2)∶460-466
[13] Tashima T, Nagatoishi S, Sagara H, et al. Osteomodulin regulates diameter and alters shape of collagen fibrils [J]. Biochem Biophys Res Commun, 2015, 463(3)∶292-296
[14] Rehn AP, Chalk AM, Wendel M. Differential regulation of osteoadherin (OSAD) by TGF-β1 and BMP-2 [J]. Biochem Biophys Res Commun, 2006, 349(3)∶1057-1064