Abstract: Objective: To study the influence of special AT-rich binding protein 2 (Satb2) overexpressionon the proliferation and migration of human dental pulp stem cells (hDPSCs). Methods: Lentiviral-mediated gene was used to transfer Satb2 overexpression into hDPSCs, while control groups were normal hDPSCs and hDPSCs infected with empty vector. Cellular proliferation was determined by CCK8 assay. Immunofluorescence staining was performed to observe the structure of microtubules. Scratch-wound assay and migration assay were used to detect the migration of Satb2-overexpressing hDPSCs. Results: After 1, 3, 5, 7 days of cultivation, the optical density (OD) in the Satb2-overexpressing hDPSCs group was significantly higher than that in the control groups (P＜0.05). The microtubules in Satb2-overexpressing hDPSCs group were more slender than those in the control groups. Scratch-wound and migration assay showed that overexpression of Satb2 promoted the migration of hDPSCs. Conclusion: Overexpression of Satb2 promoted the proliferation and migration of hDPSCs.

Key words: Special , AT-rich binding protein 2, Human dental pulp stem cells , Proliferation, Migration