Effects of Integrin α6 on Cell Adhesion in Human Dental Pulp Stem Cells under Simulated Microgravity.

doi:10.13701/j.cnki.kqyxyj.2016.04.011

Abstract

Abstract: Objective: To study the effects of integrin α6 on cell adhesion of human dental pulp stem cells seeded on PLGA scaffolds under simulated microgravity. Methods: hDPSCs were seeded on PLGA scaffolds and cultured under the conditions of normal gravity or simulated microgravity for 72 hours. The protein expression level of integrin α6, integrin αv, integrin β1, FAK and phospho-FAK were measured by Western blot. Integrin α6 siRNA was transfected into hDPSCs and DAPI immunofluorescence staining was performed to detect the adhesion ability of hDPSCs. Western blot was conducted to detect the related-protein expression levels. Results: Expression of integrin α6 and phospho-FAK were up-regulated in the microgravity group (P＜0.05) compared to control group, while there were no statistical differences in the expression levels of integrin αv, integrin β1 and FAK. Moreover, adhesion ability and phospho-FAK expression of hDPSCs were decreased after transfected with integrin α6 siRNA (P＜0.05), while there were no statistical differences in the expression levels of integrin β1 and FAK. Conclusion: Increased adhesion ability of hDPSCs seeded on PLGA scaffolds may be associated with the up-regulation of integrin α6 and its downstream molecule FAK.

CLC Number:

R780.1

YANG Dian-song, PAN Shuang, HE Li-na, LI Yan-ping, ZHANG Lin, NIU Yu-mei.. Effects of Integrin α6 on Cell Adhesion in Human Dental Pulp Stem Cells under Simulated Microgravity.[J]. Journal of Oral Science Research, 2016, 32(4): 361-364.

References

[1] Suh H. Tissue restoration, tissue engineering and regenerative medicine [J]. Yonsei Med J,2000,41(6)∶681-684
[2] L.He, S.Pan, Y.Li, et al. Increased proliferation and adhesion properties of human dental pulp stem cells in PLGA scaffolds via simulated microgravity [J]. Int Endod J, 2015 Feb 19. doi: 10.1111/iej.12441
[3] Takeda M, Magaki T, Okazaki T,et al.Effects of simulated microgravity on proliferation and chemosensitivity in malignant glioma cells [J]. Neurosci Lett, 2009,463(1)∶54-59
[4] Plett PA, Frankovitz SM, Abonour R, et al. Proliferation of human hematopoirtic bone marrow cells in microgravity [J]. In Vitro Cell Dev Biol Anim, 2001,37(2)∶73-78
[5] Cerwinka WH, Sharp SM, Boyan BD, et al. Differentiation of human mesenchymal stem cell spheroids under microgravity conditions [J] Cell Regen (Lond) ,2012 Jun 28;1(1):2. doi: 10.1186/2045-9769-1-2
[6] Zhu Q, Safavi KE, Spangberg LS. Integrin expression in human dental pulp cells and their role in cell attachment on extracellular matrix proteins [J]. J Endod,1998,24(10)∶641-644
[7] Yun HM, Lee ES, Kim MJ, et al. Magnetic Nanocomposite Scaffold-Induced Stimulation of Migration and Odontogenesis of Human Dental Pulp Cells through Integrin Signaling Pathways [J]. PLoS One, 2015 Sep 18;10(9)∶e0138614. doi: 10.1371
[8] Giancotti FG, Ruoslahti E. Integrin Signaling [J]. Science, 1999, 285(5430)∶1028-1032
[9] Geiger B, Bershadsky A, Pankov R, et al. Transmembrane extracellular matrix cytoskeleton crosstalk [J]. Nat Rev Mol Cell Biol,2001,2(11)∶793-805
[10] Teo BK, Wong ST, Lim CK, et al. Nanotopography modulates mechanotransduction of stem cells and induces differentiation through focal adhesion kinase [J]. ACS Nano, 2013, 25, 7(6)∶4785-4798
[11] Lwigale PY, Thurmond JE, Norton WN, et al.Simulated microgravity and hypergravity attenuate heart tissue [J]. Cells Tissues Organs, 2000, 167(2-3)∶171-183
[12] Guignandon A, Lafage-Proust MH , Usson Y, et al. Cell cycling determines integrin-mediated adhesion in osteoblastic ROS 17/2.8 cells exposed to space-related conditions [J]. FASEB J,2001,15(11)∶2036-2038

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