Expression of STAT6 in Salivary Gland Adenoid Cystic Carcinoma and Its Effect on Cell Proliferation and Invasion

doi:10.13701/j.cnki.kqyxyj.2018.11.016

Abstract

Abstract: Objective: To investigate the expression of STAT6 in salivary gland adenoid cystic carcinoma (SACC), and the effects of down-regulation of STAT6 gene expression on cell proliferation and invasion. Methods: A total of 53 cases of SACC were selected. And 40 cases of normal gland tissues from benign parotid gland tumors were taken as the control group. Immunohistochemical method was used to detect the expressions of STAT6 proteins in SACC and adjacent tissues. The ACC-2 cells were cultured and divided into STAT6 interference sequence group, negative control group, and blank group. Small interfering RNA (siRNA) technology was used to down-regulate expression of STAT6 gene of cells in STAT6 interference sequence group. Real-time fluorescence quantitative PCR was used to detect the expression of STAT6 gene in cells. MTT colorimetric assay was used to detect cell proliferation. Transwell assay was used to detect cell migration and invasion. Results: The positive expression rate of STAT6 protein in SACC was 71.70%, which was higher than that of control group (P=0.000). The positive expression of STAT6 protein in SACC was related to TNM stage, lymph node metastasis, and distant metastasis (P<0.05). The relative expression level of STAT6 mRNA in cells in STAT6 interference sequence group was lower than that in negative control group and blank group (P<0.05). The absorbance values at 24-96h of cells in STAT6 interference sequence group was lower than those in negative control group and blank group (P<0.05). The number of migration cells and the number of invasive cells in STAT6 interference sequence group were lower than those in negative control group and blank group (P<0.05). Conclusion: The expression of STAT6 protein in the SACC tissues was high. Down-regulation of STAT6 gene expression could inhibit the cell proliferation, migration, and invasion.

Key words: Salivary gland adenoid cystic carcinoma, Signal transducer and activator of transcription 6, Cell proliferation, Cell invasion

PEI Hao, XIA Dong-jing, HUANG Ying-ying. Expression of STAT6 in Salivary Gland Adenoid Cystic Carcinoma and Its Effect on Cell Proliferation and Invasion[J]. Journal of Oral Science Research, 2018, 34(11): 1212-1216.

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