Effects of Titania Nanotubes with Different Diameters on Bone Marrow Mesenchymal Stem Cells.

doi:10.13701/j.cnki.kqyxyj.2017.07.012

Abstract

Abstract: Objective:To investigate the effects of titania nanotubes (TNTs) with different diameters on bone marrow mesenchymal stem cells (BMSCs). Methods: TNTs with three different diameters were fabricated on Ti surfaces through electrochemical anodization. MTT method was used to determine the cell adhesion and cell proliferation abilities. Alkaline phosphatase activity, collagen secretion, and extracellular matrix mineralization were used to evaluate the osteogenic differentiation of BMSCs. Results: TNTs with a diameter of 30 nm significantly promoted the adhesion and proliferation of BMSCs at the early stage, while there was no obvious difference at the later stage. TNTs with a diameter of 200 nm showed the best ability to promote the osteogenic differentiation. However, it clearly impaired cell adhesion and proliferation. The initial cell adhesion and cell proliferation on TNTs with a diameter of 100 nm were slightly repressed. Furthermore, they could promote the osteogenic differentiation. Conclusion: The optimal diameter of TNTs for BMSCs migth be 100nm.

Key words: Titania nanotubes , Bone marrow mesenchymal stem cells , Cell adhesion , Cell proliferation, Osteogenic differentiation

CLC Number:

R783.1

XU Zhi-qiang,HUANG Si-jia,HE Yu-qi,ZOU Geng-sen,QIU Zhu-wen,CHEN Jiang.. Effects of Titania Nanotubes with Different Diameters on Bone Marrow Mesenchymal Stem Cells.[J]. Journal of Oral Science Research, 2017, 33(7): 737-741.

References

[1] Nair M, Elizabeth E. Applications of Titania Nanotubes in Bone Biology [J]. Journal of Nanoscience & Nanotechnology, 2015, 15(15)∶939-955
[2] Lord MS, Foss M, Besenbacher F. Influence of nanoscale surface topography on protein adsorption and cellular response [J]. Nano Today,2010,5(1)∶66-78
[3] Jin S. TiO2nanotubes for bone regeneration [J]. Trends in biotechnology, 2012, 30(6)∶315-322
[4] Park J, Bauer S, Von dMK, et al. Nanosize and vitality: TiO2 nanotube diameter directs cell fate [J]. Nano Letters, 2007, 7(6)∶1686-1691
[5] Oh S, Jin S. Stem cell fate dictated solely by altered nanotube dimension [J]. Proceedings of the National Academy of Sciences,2009,106(7)∶2130-2135
[6] 梁建鹤,肖秀峰,刘榕芳,等.宽电压范围下阳极氧化制备TiO2纳米管阵列及其热稳定性[J].无机化学学报,2010,26(1)∶112-119
[7] Lan MY, Liu CP, Huang HH, et al. Diameter-sensitive biocompatibility of anodic TiO2 nanotubes treated with supercritical CO₂ fluid [J]. Nanoscale Research Letters,2013,8(1)∶1-8
[8] Neoh KG, Hu X, Zheng D, Kang ET. Balancing osteoblast functions and bacterial adhesion on functionalized titanium surfaces [J]. Biomaterials,2012,33(10)∶2813-2822
[9] Wang Y, Wen C, Hodgson P, et al. Biocompatibility of TiO2 nanotubes with different topographies [J]. Journal of Biomedical Materials Research Part A, 2014, 102(3)∶743-751
[10] Minagar S, Li Y, Berndt CC, et al. The influence of titania-zirconia-zirconium titanate nanotube characteristics on osteoblast cell adhesion [J]. Acta Biomaterialia,2015,12(1)∶281-289
[11] 王祝愉, 张晓磊.骨组织细胞传递生物力学刺激的信号通路的研究进展[J].口腔医学研究,2015,31(10)∶1050-1052
[12] Kilian KA, Bugarija B, Lahn BT, et al. Geometric cues for directing the differentiation of mesenchymal stem cells [J]. Proceedings of the National Academy of Sciences of the United States of America, 2010, 107(11)∶4872-4877
[13] Wall I, Donos N, Carlqvist K, et al. Modified titanium surfaces promote accelerated osteogenic differentiation of mesenchymal stromal cells in vitro [J]. Bone, 2009, 45(1)∶17-26
[14] Cavalcantiadam EA, Aydin D, Hirschfeldwarneken VC, et al. Cell adhesion and response to synthetic nanopatterned environments by steering receptor clustering and spatial location [J]. Advanced Online Publication Articles for Hfsp Journal, 2008, 2(5)∶276-285

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