Expression of Periostin Gene in Osteogenic Differentiation of Bone Marrow Mesenchymal Stem Cells in Inflammatory Microenvironment

doi:10.13701/j.cnki.kqyxyj.2020.12.015

Abstract

Abstract: Objective: To investigate the expression level of Periostin gene in osteogenic differentiation of wistar rat bone marrow mesenchymal stem cells (rBMSCs) in inflammatory microenvironment. Methods: rBMSCs were isolated and cultured by whole bone marrow adherence method. The cell morphology was observed and the osteogenic differentiation of the third generation rBMSCs was induced. The cell inflammatory response was induced by 10ng/mL TNF-α. Then, the cell proliferation activity was detected by CCK-8 and the gene expression levels of IL-1β and TNF-α were measured by real-time quantitative PCR. The third generation of rBMSCs was divided into control group and inflammation group for osteogenic induction. The deposition amounts of calcium ions were measured after alizarin red staining. The expression levels of Periostin in the cells were detected by qRT-PCR and Western blot on the 14th day of osteogenic induction. Results: rBMSCs were successfully isolated and cultured by whole bone marrow adherence method. The gene expression levels of TNF-α and IL-1β in the inflammation group were 1.8 times (P<0.05) and 2.1 times (P<0.01) of those of control group, respectively. Compared with the control group, the inflammation group had lower bone formation ability and lower expression of related osteogenic genes. The expression levels of Periostin gene and protein in the inflammation group after osteoinduction were significantly lower than those in the control group (P<0.05). Conclusion: The expression level of Periostin gene in osteogenic differentiation of rBMSCs induced by inflammatory microenvironment decreased, and the osteogenic capacity of rBMSCs decreased.

Key words: bone marrow mesenchymal stem cells, inflammation, osteogenic differentiation, Periostin

LU Yongchao, WANG Wei, LV Ao, LIU Yiqing, DU Yi. Expression of Periostin Gene in Osteogenic Differentiation of Bone Marrow Mesenchymal Stem Cells in Inflammatory Microenvironment[J]. Journal of Oral Science Research, 2020, 36(12): 1148-1152.

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