Antibacterial Properties of Titania Nanotubes with Different Diameters

doi:10.13701/j.cnki.kqyxyj.2020.03.010

Abstract

Abstract: Objective: study the antibacterial properties of titania nanotubes with three different diameters. Methods: Titania nanotubes with three different diameters were fabricated on titanium surfaces through electrochemical anodization at 10, 30, and 60 V (denoted as NT10, NT30, and NT60). The sample topography was assessed with SEM. The crystalline phase analysis was conducted using X-ray diffractomer. Contact angle measurements were examined using a contact angle measuring system, and roughness was examined using atomic force microscope. The antibacterial properties of titania nanobues were evaluated by in vitro co-culture with Staphylococcus aureus and Porphyromonas Gingivalis. The live bacteria were observed with SEM and quantitated by the plate-counting method. Results: The diameters of titania nanotubes were about 30 nm (NT10), 100 nm (NT30), and 200 nm (NT60), respectively. All three types of nanotubes had anatase peaks as indicated by X-ray diffractomer. The contact angles on nanotubes were smaller than those on the control titanium, and reached the lowest on NT60. The roughness values of the nanotubes were smaller than those of control titanium, and reached the lowest on NT30. The bacterial on the titania nanotubes were significantly decreased when compared to the titanium. The adhesion of Staphylococcus aureus reached the lowest on NT60, and the adhesion of Porphyromonas Gingivalis reached the lowest on NT30. Conclusion: The titania nanotubes fabricated on the surface of titanium all inhibited the adhesion of bacteria to some extent.

Key words: titania nanotubes, antibacterial properties, staphylococcus aureus, porphyromonas gingivalis

XU Zhiqiang, ZENG Xiufeng, HONG Shaonan, ZENG Xiuxia, HE Yuqi, HUANG Junhui. Antibacterial Properties of Titania Nanotubes with Different Diameters[J]. Journal of Oral Science Research, 2020, 36(3): 230-234.

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