Abstract: Objective: To prepare antibacterial periodontal guided tissue regeneration membrane by grafting 2-methacryloyloxyethyl phosphorylcholine (2-MPC) onto PLGA film. Methods: 2-MPC was grafted to PLGA surface by ultraviolet induced grafting polymerization. Fourier-transform infrared (FTIR) was used to confirm the success of grafting. The surface morphology of the samples was observed by scanning electron microscopy (SEM). The samples were fixed with 2.5% glutaraldehyde for 10 h after incubating with E. coli and S. aureus at a concentration of 1×10⁶ CFU/mL (in PBS) for 4 h. And then the samples were dehydrated in graded series of ethanol solutions, dried at room temperature, coated with gold, and examined by SEM. In addition, mouse osteoblastic cell line MC3T3-E1 was seeded on the surface of the material and cultured for 12 h. The living cell staining was conducted to observe the cell morphology on the surface of the samples. Results: FTIR and SEM showed that 2-MPC was successfully grafted on the PLGA membrane. The grafted materials possessed excellent resistance to E. coli and S. aureus, and MPC did not significantly influence the biocompatibility of the materials in vitro. Conclusion: PLGA grafted with 2-MPC could be used as a kind of periodontal tissue guided regeneration membrane which has antibacterial property and biocompatibility.

Key words: regeneration membrane of periodontal guide tissue, 2-methacryloxyethyl phosphatidylcholine, poly(lactic-co-glycolic acid) copolymer, Escherichia coli, Staphylococcus aureus