Effect and Mechanism of Astragaloside on Regulating the Inflammatory Response and Osteogenic Differentiation of Human Periodontal Ligament Cells

doi:10.13701/j.cnki.kqyxyj.2020.02.008

Abstract

Abstract: Objective: To observe the effect and mechanism of astragaloside on regulating the inflammatory response and osteogenic differentiation of human periodontal ligament cells (hPDLCs) infected by Porphyromonas gingivalis through NLRP3 inflammasome. Methods: Primary hPDLCs were cultured and digested, and the third generation of hPDLCs was divided into control group, infection group, astragaloside group, astragaloside+ NLR family, and pyrin domain containing 3 (NLRP3) overexpression group. Interleukin (IL)-1β, IL-18, alkaline phosphatase (ALP), NLRP3, cysteinyl aspartate specific proteinase-1 (Caspase-1), Runt-related transcription factor (RUNX2), and osteopontin (OPN) in cells were detected. Alizarin red staining was performed after mineralization induction to observe the mineralized nodules. Results: The contents of IL-1β and IL-18 in the culture medium and the expressions of IL-1β, IL-18, NLRP3, and Caspase-1 in the cells of the astragaloside group were significantly lower than those of the infection group. The activity of ALP in the culture medium, the expression of RUNX2 and OPN in the cells, and the number of mineralized nodules were higher than those of the infection group. The contents of IL-1β and IL-18 in the culture medium and the expressions of IL-1β, IL-18, NLRP3, and Caspase-1 in the cells of astragaloside+NLRP3 overexpression group were significantly higher than those of the astragaloside group. The activity of ALP in the culture medium, the expressions of RUNX2 and OPN in the cells, and the number of mineralized nodules were lower than those of the astragaloside group. Conclusion: Astragaloside can inhibit the inflammatory response of porphyromonas gingivalis infected hPDLCs and promote the osteogenic differentiation by inhibiting the activation of NLRP3 inflammasome.

Key words: periodontal ligament cells, porphyromonas gingivalis, astragaloside, NLR family, pyrin domain, containing 3 inflammasome, inflammatory response, osteogenic differentiation

WANG Suwen, LAI Siyu, XI Lijun, WANG Lihong. Effect and Mechanism of Astragaloside on Regulating the Inflammatory Response and Osteogenic Differentiation of Human Periodontal Ligament Cells[J]. Journal of Oral Science Research, 2020, 36(2): 121-125.

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