Effects of D/L-valine on Porphyromonas Gingivalis and Porphyromonas Gingivalis Biofilm

doi:10.13701/j.cnki.kqyxyj.2020.07.010

Abstract

Abstract: Objective: To study the effects of D-valine and L-valine on porphyromonas gingivalis (P.gingivalis) and its biofilms. Methods: The concentration of D-valine without cytotoxicity was obtained by CCK-8 experiment. The effects of D-valine and L-valine on the bacterial activity of P.gingivalis were evaluated by growth curve experiment. The inhibition of D-valine and L-valine on the biofilm formation of P.gingivalis and the dispersion of mature P.gingivalis biofilm were evaluated by crystal violet staining experiment, extracellular polysaccharide experiment, and scanning electron microscope. Results: D-valine had no cytotoxicity to fibroblasts at 50 mmol/L and 75 mmol/L concentrations. D-valine (75 mmol/L) could effectively inhibit the bacterial activity and biofilm formation of P.gingivalis and destroyed the mature P.gingivalis biofilm. D-valine (50 mmol/L) inhibited the bioactivity and biofilm formation of P.gingivalis for 72 h. L-valine (75 mmol/L and 50 mmol/L) promoted the bacterial activity of P.gingivalis and the formation of biofilm. When D-valine and L-valine existed simultaneously, D-valine functions were shown only. Conclusion: D-valine can inhibit the bacterial activity and biofilm formation of P.gingivalis and destroy the mature biofilm. L-valine can promote the bacterial activity and biofilm formation of P.gingivalis, but it is not antagonistic to D-valine. D-valine can be used as a potential biologic agent to treat bacterial plaque related diseases.

Key words: D-amino acid, D-valine, porphyromonas gingivalis, biofilm

ZHANG Huiyan, LI Baosheng, ZHANG Zhenyang, HAN Chunyu, LI Zuntai, QI Xiaoshuang, FU Qiyue, MENG Weiyan. Effects of D/L-valine on Porphyromonas Gingivalis and Porphyromonas Gingivalis Biofilm[J]. Journal of Oral Science Research, 2020, 36(7): 648-653.

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