Role of Streptococcus Sanguis Bacteriocin on Regulating the Activity of Biofilms of Aggregatibacter Actinomycetemcomitans and Candida Albicans

doi:10.13701/j.cnki.kqyxyj.2020.04.013

Abstract

Abstract: Objective: To study the MIC of streptococcus sanguis bacteriocin on the biofilm of A.a and C.a. and to study the effects of Streptococcus sanguis bacteriocin on the biofilm activity of A.a and mixed cultured A.a and C.a. by laser confocal microscopy. Methods: Through low temperature high speed centrifugation, cell fragmentation, salting out, and other methods, Streptococcus sanguis bacteriocin was extracted. The MIC of A.a and mixed cultured A.a and C.a were determined by double dilution method. The biofilm activity was observed after 2h, 6h, 12h, 24h, 48h, and 72h by laser confocal microscope after the action of Streptococcus sanguis bacteriocin on A.a and mixed cultured A.a and C.a. Results: The MIC of separate A.a was 0.25 g/L. The MIC of separate C.a was 0.125 g/L. The MIC of the mixed cultured A.a and C.a was 0.5 g/L . The results of laser confocal microscope showed that the biofilm activity of A.a was strongly inhibited by streptococcus sanguis bacteriocin. The most significant time was 72 h. The biofilm activity of mixed culture A.a and C.a were also strongly inhibited by Streptococcus sanguis bacteriocin. The most significant time was 48 h. Conclusion: Streptococcus sanguis bacteriocin inhibited the biofilm activity of separate A.a and the mixed cultured A.a and C.a.

Key words: streptococcus sanguis bacteriocin, aggregatibacter actinomycetemcomitans, candida albicans, biofilm

WU Yinxuan, MA Shengli, GE Wenyu, LI Hui, WU Lichun, WANG Xin, LI Chunmei. Role of Streptococcus Sanguis Bacteriocin on Regulating the Activity of Biofilms of Aggregatibacter Actinomycetemcomitans and Candida Albicans[J]. Journal of Oral Science Research, 2020, 36(4): 360-364.

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