Expression of IL-17 in Chronic Periapical Periodontitis of Deciduous Teeth

doi:10.13701/j.cnki.kqyxyj.2020.04.014

Abstract

Abstract: Objective: To detect the expression and distribution of IL-17 in periapical lesions of deciduous teeth and investigate the possible role of IL-17 in the pathogenesis of chronic periapical periodontitis in deciduous teeth. Methods: Tissue samples were collected from 120 deciduous teeth with chronic periapical periodontitis. Histopathological examination was performed to determine the pathological type and the degree of inflammatory cell infiltration. The distribution characteristics and expressions of IL-17 in tissues were detected by immunohistochemistry and ELISA. Results: Periapical granulomas (65.8%) were the most common pathological type in 120 deciduous teeth, followed by periapical cyst (18.4%) and periapical abscess (15.8%). IL-17 was expressed in three pathological types, mainly in lymphocytes and plasma cells. ELISA results showed that the expressions of IL-17 in three pathological types were lower than that in control group and the degree of inflammation was negatively correlated with IL-17 in periapical granulomas. Conclusion: IL-17 was detected widely in periapical lesions of deciduous teeth. Expression of IL-17 gradually decreased with the inflammation progression. IL-17 may contribute to the inhibition of the progression in chronic periapical periodontitis.

Key words: IL-17, deciduous teeth, chronic periapical periodontitis, periapical granuloma

ZHOU Yan, WANG Yinghui, ZHAO Huanying, SHANG Jiajian.. Expression of IL-17 in Chronic Periapical Periodontitis of Deciduous Teeth[J]. Journal of Oral Science Research, 2020, 36(4): 365-368.

References

[1] Gupta SS, Shetty DC, Urs AB, et al. Role of inflammation in developmental odontogenic pathosis [J]. J Oral Maxillofac Pathol, 2016, 20(1):164.
[2] Márton IJ, Kiss C. Protective and destructive immune reactions in apical periodontitis [J]. Oral Microbiol Immunol, 2000, 15(3):139-150.
[3] 杨栋,李红梅,刘林花,等.TH17及其相关因子在不同牙周炎症状龈沟液中的检测[J].口腔医学研究,2017,33(11):1161-1164.
[4] 吴凯.IL-17和牙周膜成纤维细胞对体外诱导破骨样细胞生成的影响[D].广西医科大学,2017.
[5] Tsai CH, Weng SF, Yang LC, et al. Immunohistochemical localization of tissue-type plasminogen activator and type I plasminogen activator inhibitor in radicular cysts [J]. J Oral Pathol Med, 2004, 33(3): 156-161.
[6] Cua DJ, Tato CM. Innate IL-17-producing cells: the sentinels of the immune system [J]. Nat Rev Immunol, 2010, 10(7): 479-489.
[7] Gaffen SL, Jain R, Garg AV, et al. The IL-23-IL-17 immune axis: from mechanisms to therapeutic testing [J]. Nat Rev Immunol, 2014, 14(9): 585-600.
[8] Mirkovi S, Tadi A, Durdevi Mirkovi T, et al. Comparative analysis of accuracy of diagnosis of chronic periapical lesions made by clinical and histopatological examination [J]. Med Pregl, 2012, 65(7-8): 277-280.
[9] Pereira Faustino IS, Azevedo RS, Takahama AJr. Metalloproteinases 2 and 9 immunoexpression in periapical lesions from primary endodontic infection: possible relationship with the histopathological diagnosis and the presence of pain [J]. J Endod, 2016, 42(4):547-551.
[10] 纪志勇,张佳莉,孟柳燕. 383例根尖周病变的临床病理系统性回顾与分析[J].口腔医学研究, 2013, 29(10) :978-981.
[11] Matsuda V, Kadowaki ACN, Kataoka SHH, et al. A comparison of clinical, histological and radiographic findings in periapical radiolucid lesions [J]. Dental Press Endod, 2011, 1(3): 17-21.
[12] 罗俊.细胞因子IL-17A在牙根吸收过程中表达的实验研究[D].南昌大学,2012.
[13] Ferreira LG, Rosin FC, Corrêa L. Analysis of Interleukin 17A in periapical abscess and granuloma lesions[J]. Braz Oral Res, 2016, 30(1):e34.
[14] Alshwaimi E, Berggreen E, Furusho H, et al. IL-17 receptor a signaling is protective in infection-stimulated periapical bone destruction [J]. J Immunol, 2013, 191(4):1785-1791.
[15] Ono T, Okamoto K, Nakashima T, et al. IL-17-producing γδ T cells enhance bone regeneration [J]. Nat Commun, 2016, 7:10928.
[16] Gladiator A, Wangler N, Trautwein-Weidner K, et al. Cutting edge: IL-17-secreting innate lymphoid cells are essential for host defense against fungal infection [J]. J Immunol, 2013, 190(2): 521-525.
[17] Liu J, Qu H, Li Q, et al. The responses of γδ T-cells against acute Pseudomonas aeruginosa pulmonary infection in mice via interleukin-17 [J]. Pathog Dis, 2013, 68(2):44-51.
[18] Jie Z, Liang Y, Hou L, et al. Intrahepatic innate lymphoid cells secrete IL-17A and IL-17F that are crucial for T cell priming in viral infection [J]. J Immunol, 2014, 192(7):3289-3300.