Scavenging Effects of Nisin on Enterococcus faecalis Biofilm

doi:10.13701/j.cnki.kqyxyj.2020.07.007

Abstract

Abstract: Objective: To explore the scavenging effect of different concentrations of Nisin on Enterococcus faecalis biofilm. Methods: A 24h-old biofilm model of Enterococcus faecalis was established in vitro. The MTT method was applied to evaluate the scavenging effect of Nisin (A1, B1, C1, D1, E1) on the 24h-old Enterococcus faecalis biofilm. The confocal laser scanning microscopy was used to observe the effect of the tested drugs (A2, B2, C2, D2, E2) on the structures of Enterococcus faecalis biofilms in vitro. Results: Nisin of different concentrations scavenged the biofilms to some extent (P<0.05), yet with higher biofilm biomass than that of PC1 (P<0.05). There were different statistical differences when the experimental groups compared within them. There were statistically significant differences between any two in the experimental groups (P<0.05), except for between C1 and D1 (P>0.05). The images of CLSM showed Nisin could damage the biofilm significantly, leaving the biofilm structure becoming sparse. Conclusion: Nisin could scavenge and disrupt the E.faecalis biofilm to some extent.

Key words: Nisin, Enterococcus faecalis, biofilm

ZHANG Xuyang, LI Hui, LIU Yi, LOU Tingting, BAO Xueying, LI Shiya, HUANG Hui. Scavenging Effects of Nisin on Enterococcus faecalis Biofilm[J]. Journal of Oral Science Research, 2020, 36(7): 635-638.

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