Preliminary Study on Dual-species Biofilm of Enterococcus faecalis and Streptococcus mutans.

doi:10.13701/j.cnki.kqyxyj.2020.12.011

Abstract

Abstract: Objective: To measure the biomass and observe the structure of dual-species biofilm of Streptococcus mutans UA140-mrfp and Enterococcus faecalis ATCC 29212 using BioFlux System. Methods: Biofilm volume was measured by crystalline violet staining after mixed culture of Streptococcus mutans and Enterococcus faecalis. After 30 min standing, dual-species biofilm was cultured in BioFlux200 system under 0.8 dyne continuous shear force for 12 hours. The biofilm structure was observed under fluorescence microscope. Results: The volume of dual-species biofilm was not statistically different from that of mono-species biofilm. However, the dual-species biofilm could form reticulated distribution biofilms in microfluidic environment. Conclusion: Compared with static biofilm models, BioFlux system could form integrated dual-species biofilm.

Key words: microfluidic, biofilm, Streptococcus mutans, Enterococcus faecalis

ZHANG Mengjie, LU Wei, HOU Mengyao, JIANG Xiu, WANG Feihu, TAO Rui. Preliminary Study on Dual-species Biofilm of Enterococcus faecalis and Streptococcus mutans.[J]. Journal of Oral Science Research, 2020, 36(12): 1128-1131.

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