Anti-caries Effect of A Bacteriophage Lysin.

doi:10.13701/j.cnki.kqyxyj.2018.10.006

Abstract

Abstract: Objective: To evaluate the bactericidal activity of a bacteriophage lysin ClyR on the clinical strains of streptococcus mutans (S. mutans) and streptococcus sobrinus (S. sobrinus). Methods: Dental plaques of children with severe early childhood caries (SECC) were obtained and cultured for S. mutans and S. sobrinus isolation. Morphology observation, biochemistry identification, and a 16S rDNA sequencing analysis were used to identify all clinical isolates. The bactericidal activity of ClyR on S. mutans and S. sobrinus isolates were determined by turbidity decrease assay and minimum bactericidal concentration (MBC) assay. Transmission electron microscopy (TEM) was used to visualize the effects of ClyR on the bacterial cell wall. Results: 10 S. mutans and 10 S. sobrinus were randomly selected from the isolates to test the susceptibility to ClyR. 8 of 10 S. mutans isolates and 3 of 10 S. sobrinus isolates were susceptible to ClyR, with MBC ranging from 125 mg/L to >1000 mg/L and 125 mg/L to 500 mg/L, respectively. TEM showed that ClyR could degrade the bacteria cell wall, which resulted the osmotic death. Conclusion: ClyR exhibits strong lytic activity against both S. mutans and S. sobrinus isolates, but the S. mutans is better than S. sobrinus, which represents a preventative or therapeutic agents against dental caries.

Key words: Streptococcus mutans, Streptococcus sobrinus, Clinical isolates, Bacteriophage lysin, Dental caries

XU Jing-jing, TAO Ting-liang, LI Yu-hong.. Anti-caries Effect of A Bacteriophage Lysin.[J]. Journal of Oral Science Research, 2018, 34(10): 1052-1056.

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