Berberine Stimulates Osteogenesis/Odontogenic Differentiation of Human Dental Pulp Stem Cells

doi:10.13701/j.cnki.kqyxyj.2020.07.008

Abstract

Abstract: Objective: To investigate the effect of berberine on proliferation and osteogensis/odontogenic differentiation of human dental pulp stem cells. Methods: Human dental pulp stem cells (hDPSCs) were isolated and cultured. The phenotype was identified by flow cytometry. hDPSCs were treated with different concentrations of berberine (0, 1, 3, 10, and 30 μmol/L). Cell proliferation and osteogenesis/odontogenic differentiation were detected by CCK-8, ALP activity assay, and q-PCR. Results: hDPSCs were successfully isolated and identified. CCK-8 results showed that within 7 days of culture, berberine at 0-10 μmol/L concentration had no effect on hDPSCs proliferation. However, 30 μmol/L group inhibited cell proliferation at day 7. ALP activity assay detected that berberine significantly promoted the activity of alkaline phosphatase at the concentration of 1 and 3μmol/L. q-PCR also demonstrated that berberine could up-regulate the mRNA expression of genes related to osteogenesis and odontogenic, and the promotion effect was most significant when the concentration was 1 and 3 μmol/L. Conclusion: Berberine can promote osteogenesis/odontogenic differentiation of hDPSCs at 1-3 μmol/L.

Key words: berberine, human dental pulp stem cells, osteogenesis differentiation, odontogenic differentiation

MA Lan, YU Yijun, LIU Hanxiao, LIU Chao, MIAO Leiying. Berberine Stimulates Osteogenesis/Odontogenic Differentiation of Human Dental Pulp Stem Cells[J]. Journal of Oral Science Research, 2020, 36(7): 639-643.

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