Study on Expression of Phospholipase Cγ in Inflammatory Microenvironment of Enterococcus Faecalis Lipoteichoic Acid

doi:10.13701/j.cnki.kqyxyj.2019.11.018

Abstract

Abstract: Objective: To explore the expression of PLCγ and NFATC1 mRNA in osteoblasts and osteoclasts under the inflammatory microenvironment of Enterococcus faecalis LTA. Methods: Mouse RAW264.7 and MC3T3-E1 were induced into osteoclasts and osteoblasts, respectively. Immunofluorescence was used to observe the localization of PLCγ and NFATC1 after LTA treatment of osteoclasts and osteoblasts. Real-time PCR was used to detect the expression of PLCγ and NFATC1 mRNA after osteoclast and osteoblasts by LTA. CCK8 detects the proliferative activity of LTA after osteoclast and osteoblasts. Results: Osteogenic and osteoblasts were successfully induced. Immunofluorescence showed PLCγ localization in the cytoplasm and NFATC1 localization in the nucleus. PCR results showed that the mRNA expression levels of PLCγ and NFATC1 in osteoblasts were significantly decreased after LTA stimulation (P<0.05). The mRNA expression levels of PLCγ and NFATC1 in osteoclasts were significantly increased after LTA stimulation (P<0.05). Conclusion: PLCγ and NFATC1 may play a role in the inflammatory response and bone destruction of refractory apical periodontitis.

Key words: osteoclasts, osteoblasts, lipoteichoic acid, enterococcus faecalis, phospholipaseCγ

SHI Dongmei, XU Huijun, ZUO Meina, ZHANG Hong, ZHANG Mi, WU Saixuan, DONG Ming, NIU Weidong. Study on Expression of Phospholipase Cγ in Inflammatory Microenvironment of Enterococcus Faecalis Lipoteichoic Acid[J]. Journal of Oral Science Research, 2019, 35(11): 1084-1088.

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