Journal of Oral Science Research

Journal of Oral Science Research ›› 2017, Vol. 33 ›› Issue (12): 1286-1290.DOI: 10.13701/j.cnki.kqyxyj.2017.12.012

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Study on Bacteriostasis of Sodium Aescinate-loaded Nanomesoporous Silica on Enterococcus Faecalis.

HUANG Da-wei1, LI Feng-hua2*, LEI Zhuo2, JIANG Hai-min3.   

  1. 1. Department of Stomatology, Maternal and Child Health Care Hospital of Xiamen, Xiamen 361003, China;
    2. Xiangya School of Stomatology, Central South University, Changsha 410008, China;
    3. Department of Stomatology, Zhongshan Hospital, Xiamen University, Xiamen 361003, China.
  • Received:2017-05-25 Online:2017-12-20 Published:2018-01-03

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Abstract: Objective: To investigate the bacteriostasis of sodium aescinate-loaded nanomesoporous silica before and after animo-modified (SA-SBA-15 and SA-NH2-SBA-15) and calcium hydroxide (Ca(OH)2) on Enterococcus faecalis. Methods: The MIC of SA-NH2-SBA-15, SA-SNA-15, and Ca(OH)2 on Enterococcus faecalis were determined by liquid dilution susceptibility experiment. The diameter of inhibition zone on Enterococcus faecalis was observed and measured with the method of liquid diffusion. To evaluate the antibacterial effect, SA-NH2-SBA-15, SA-SBA-15, and Ca(OH)2 were cultured with bacterial solution for 168 h,and then 0.1 mL solution was removed for anaerobic cultivation at each 24h. Results: The liquid dilution test showed: the MIC of SA-NH2-SBA-15 and SA-SBA-15 was 3.5mg/mL, the MIC of Ca(OH)2 was 4.0mg/mL. When the concentrations of SA-NH2-SBA-15, SA-SBA-15 and Ca(OH)2 was higher than the MIC, the inhibition zone size increased along with the concentrations (P<0.05). From 72 h to 168 h, the CFU of SA-NH2-SBA-15 and SA-SBA-15 was less than that of Ca(OH)2. After 96 h, the CFU of SA-NH2-SBA-15 was obviously less than that of SA-SBA-15 (P<0.05). Conclusion: Three kinds of drugs had a certain inhibitory effect on Enterococcus faecalis. The bacteriostatic effect of SA-NH2-SBA-15 and SA-SBA-15 was better than that of Ca(OH)2. The efficacy of SA-NH2-SBA-15 on Enterococcus faecalis was longer than that of SA-SBA-15 and Ca(OH)2.

Key words: Mesoporous silica, Enterococcus faecalis , Sodium aescinate, Calcium hydroxide

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