Therapy of Oral Squamous Cell Carcinoma using Doxorubicin-loading Mesoporous Magnetic Silia Nanoparticles in Vitro.

doi:10.13701/j.cnki.kqyxyj.2017.08.023

Abstract

Abstract: Objective: To investigate the targeting effect of doxorubicin-loading mesoporous magnetic silia nanoparticles (DOX@M-MSNs) on oral squamous carcinomas cells in vitro. Methods: Methyl thiazolyl tetrazolium (MTT), flow cytometry, and laser scanning confocal microscope were used to detect the targeted anti-tumor activity of DOX@M-MSNs against CAL-27. Results: The blank mesoporous magnetic silia nanoparticles showed excellent biocompatibility with cell viability >80% at the maximum tested concentration. With increase of concentration and the going of time, the inhibition of tumor cell proliferation from DOX@M-MSNs was superior to that from free DOX (P<0.05). Flow cytometry showed that DOX@M-MSNs induced higher necrotic rates than free DOX, and with the elapse of time, the necrosis rate increased. Laser scanning confocal microscope showed that the magnetic area of DOX @ M-MSNs in gathered was better than that of non-magnetic regions of the tumor cells through external magnetic field, compared with non-magnetic area, the magnetic area showed a higher cell death. Conclusion: DOX@M-MSNs can selectively recognize oral squamous carcinomas cells, enhance to deliver DOX into the cells, and promote its effect in killing oral squamous carcinomas cells. By applying the magnetic field, the drug delivery efficiency of DOX@M-MSNs can be increased.

Key words: Doxorubicin , Mesoporous silica nanoparticles, External magnetic fields, TArgeting action , Tumor

CLC Number:

R739.8

XUE Ben-zhe, LI Shan-chang, YANG Shao-wei, DONG Bo, QU Xue-yan. Therapy of Oral Squamous Cell Carcinoma using Doxorubicin-loading Mesoporous Magnetic Silia Nanoparticles in Vitro.[J]. Journal of Oral Science Research, 2017, 33(8): 893-897.

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