Influence of Down-regulation of miR-181a-5p Expression on Spontaneous Cell Fusion of CAL-27 and HUVEC

doi:10.13701/j.cnki.kqyxyj.2020.08.013

Abstract

Abstract: Objective: To investigate the effect of miR-181a-5p on the fusion between oral cancer cell line CAL-27 and human vascular endothelial cells (HUVECs). Methods: The spontaneous cell fusion of CAL-27 and HUVEC was confirmed by the stable transfection of CAL-27 and HUVEC with green fluorescent protein and red fluorescent protein. The expression of miR-181a-5p in HUVEC was detected by real-time PCR. The expression of miR-181a-5p after HUVEC miR-181a-5p inhibitor infection was analyzed. The fusion was analyzed by manual counting and flow cytometry. Results: CAL-27 could fuse with HUVEC. By reducing the expression of miR-181a-5p, it was found that the fusion rate of CAL-27 and HUVEC increased (P<0.05). Conclusion: miR-181a-5p is associated with cell fusion, and the decrease of miR-181a-5p can increase the fusion of OSCC and HUVEC.

Key words: tumor, cell fusion, miR-181a-5p

HUANG Chunming, LIU Ke. Influence of Down-regulation of miR-181a-5p Expression on Spontaneous Cell Fusion of CAL-27 and HUVEC[J]. Journal of Oral Science Research, 2020, 36(8): 757-760.

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