Impact of High Glucose on Porphyromonas Gingivalis LPS induced Inflammation of Periodontal Ligament Cells

doi:10.13701/j.cnki.kqyxyj.2020.12.010

Abstract

Abstract: Objective: To investigate the impact of high glucose on inflammatory mediators release and NF-κB expression and on activation of human periodontal ligament cells induced by P.gingivalis lipopolysaccharide. Methods: Primary cultured hPDLCs were challenged in P.gingivalis LPS (1μg/mL), high glucose (25mmol/L), and high glucose+P.gingivalis LPS for 6 and 12 hours. The hPDLCs were cultured in physical concentration of glucose as control. The expression of TNF-α and IL-1β were detected by ELISA. The expression of NF-κB p65 and p-NF-κB p65 were measured by qRT-PCR and western-blot. Results: High glucose and P.gingivalis LPS could promote the expression of inflammatory cytokines TNF-α and IL-1β, and promote the expression and activation of NF-κB p65 in hPDLCs. The co-stimulation of high glucose and P.gingivalis LPS could enhance the expression of inflammatory cytokines and activation of NF-κB p65. Conclusion: High glucose may enhance the inflammation induced by P. gingivalis LPS through activation of NF-κB.

Key words: Porphyromonas gingivalis, lipopolysaccharide, high glucose, periodontitis, diabetes

SUN Tianxiang, MIAO Di, WANG Baoyan, CHEN Yue. Impact of High Glucose on Porphyromonas Gingivalis LPS induced Inflammation of Periodontal Ligament Cells[J]. Journal of Oral Science Research, 2020, 36(12): 1123-1127.

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