Abstract: Objective: To investigate the effect of BaTO₃/P(VDF-TrFE) piezoelectric nanocomposite membrane with different barium titanate contents on the adhesion, spreading, and proliferation of rat bone marrow mesenchymal stem cells to provide the basis for optimizing the biological properties of the electroactive materials. Methods: BaTO₃/P(VDF-TrFE) piezoelectric nanocomposite films containing different contents of barium titanate nanoparticles (0vol%, 1vol%, 3vol%, 5vol%) were prepared by solution casting method. The surface morphology and microstructures were characterized by scanning electron microscopy and atomic force microscopy. X-ray diffraction was used to detect the chemical composition of the materials. The ferroelectric tester was used to test the piezoelectric properties of the materials. The rat BMSCs of P3-P5 generation were cultured on BaTO₃/P (VDF-TrFE) piezoelectric nanocomposites containing different barium titanate after polarization treatment. The adhesion spread and proliferation of BMSCs were detected by scanning electron microscopy and CCK-8, respectively. SPSS13.0 software was used to analyze the statistical analysis by one-way ANOVA. Results: The piezoelectric properties of nanocomposite films showed barium titanate content dependence. Conclusion: BaTO₃/P(VDF-TrFE) piezoelectric nanocomposite films containing 5 vol% barium titanate nanoparticles can promote the adhesion, spreading, and proliferation of BMSCs.

Key words: Nanocomposite films, Piezoelectric activity, Stem cells, Cell spreading, Cell proliferation