Journal of Oral Science Research ›› 2018, Vol. 34 ›› Issue (1): 10-13.DOI: 10.13701/j.cnki.kqyxyj.2018.01.003

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Effect of Primary Cilia on Expression of Osteogenic-related Genes of Human Periodontal Ligament Cells

JIANG Si-qi1, YIN Feng-ying2, ZHANG Fan3, WANG Min1, XIA Hai-bin1*   

  1. 1. School of Stomatology, Wuhan University, Wuhan 430079, China;
    2. West China College of Stomatology, Sichuan University, Chengdu 610041, China;
    3. Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430070, China
  • Received:2017-08-03 Online:2018-01-28 Published:2018-01-26

Abstract: Objective: To study the expression of osteogenic-related genes of human periodontal ligament cells (hPDLCs) by knocking down IFT88 to inhibit the formation of primary cilia. Methods: Control shRNA and shIFT88 were infected into hPDLCs. The expression of IFT88 was detected by qRT-PCR and Western blot. The formation of primary cilia was detected by immunofluorescence and the expressions of osteogenic-related genes COL1A1, OCN, Runx2, and BMP2 were detected by qRT-PCR. Results: The PKLO.1-shIFT88 was stably expressed in hPDLCs and inhibited the expression of IFT88 effectively (P<0.01). The formation of primary cilia was partly suppressed (P<0.01). The expressions of osteogenic-related genes like COL1A1, OCN, Runx2, and BMP2 were decreased significantly (P<0.01). Conclusion: Knockdown IFT88 inhibited the formation of primary cilia and reduced the expressions of osteogenic-related genes of hPDLCs.

Key words: Intraflagellar transport 88, Primary cilia, Human periodontal ligament cells, Osteogenic-related genes

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