IL-21 Regulates RANKL Expression and Proliferation Activity in Human Periodontal Ligament Cells via Notch Signal Pathway

doi:10.13701/j.cnki.kqyxyj.2016.05.009

Abstract

Abstract: Objective: To explore the effect of interleukin-21 (IL-21) via Notch signal pathway on the expression of RANKL/OPG and proliferation in hPDLCs and to investigate the possible role of IL-21 in bone resorption of apical periodontitis. Methods: hPDLCs were treated with IL-21 in different concentrations (0, 10, 25, 50 μg/L). Then the optimal concentration (50μg/L) was used in the time-dependent experiment. The expressions of RANKL/OPG were detected by real-time PCR and ELISA. The expressions of RANKL/OPG were detected by real-time PCR and ELISA. Real-time PCR and western-blot were conducted to detect the expression of Notch1 in hPDLCs treated by IL-21. After pretreatment with GSI (5μmol/L) to inhibit Notch1 signaling pathway, the expressions of RANKL/OPG of hPDLCs treated with IL-21 (50μg/L) were investigated. CCK8 was carried out to detect the proliferation activity in the different groups of hPDLCs. Results: The expressions of RANKL were significantly up-regulated by IL-21. IL-21 decreased the cell proliferation activity in hPDLCs. Conclusion: IL-21 up-regulated the expression of RANKL and reduced proliferation activity in hPDLCs via Notch signal pathway.

CLC Number:

R780.1

XING Quan, QIN Wei, ZHANG Xiao-lei, FAN Ming-wen.. IL-21 Regulates RANKL Expression and Proliferation Activity in Human Periodontal Ligament Cells via Notch Signal Pathway[J]. Journal of Oral Science Research, 2016, 32(5): 460-463.

References

[1] NAIR P N. Pathogenesis of apical periodontitis and the causes of endodontic failures [J]. Critical reviews in oral biology and medicine : an official publication of the American Association of Oral Biologists, 2004, 15(6)∶348-381.
[2] Takayanagi H. Inflammatory bone destruction and osteoimmunology [J]. Journal of periodontal research, 2005, 40(4)∶287-293
[3] De Brito LC, Teles FR, Teles RP, et al. T-lymphocyte and cytokine expression in human inflammatory periapical lesions [J]. Journal of endodontics, 2012, 38(4)∶481-485
[4] Xiong H, Wei L, Peng B. Immunohistochemical localization of IL-17 in induced rat periapical lesions [J]. Journal of endodontics, 2009, 35(2)∶216-220
[5] Ajuz NC, Antunes H, MENDONCA T A, et al. Immunoexpression of interleukin 17 in apical periodontitis lesions [J]. Journal of endodontics, 2014, 40(9)∶1400-1403
[6] Leonard WJ, Zeng R, Spolski R. Interleukin 21: a cytokine/cytokine receptor system that has come of age [J]. Journal of leukocyte biology, 2008, 84(2)∶348-356
[7] Kwok SK, Cho ML, Park MK, et al. Interleukin-21 promotes osteoclastogenesis in humans with rheumatoid arthritis and in mice with collagen-induced arthritis [J]. Arthritis and rheumatism, 2012, 64(3)∶740-751
[8] 胡菊花,李倩,王艳青,等.白细胞介素-21和核因子κB受体活化因子配体在人根尖囊肿和肉芽肿中的表达及临床意义[J].华西口腔医学杂志,2015,33(3)∶244-248
[9] Naveh GR, Lev-tov Chattah N, Zaslansky P, et al. Tooth-PDL-bone complex: response to compressive loads encountered during mastication-a review [J]. Archives of oral biology, 2012, 57(12)∶1575-1584
[10] Li Y, Zheng W, Liu J S, et al. Expression of osteoclastogenesis inducers in a tissue model of periodontal ligament under compression [J]. J Dent Res, 2011, 90(1)∶115-120
[11] Heymann MF, Herisson F, Davaine JM, et al. Role of the OPG/RANK/RANKL triad in calcifications of the atheromatous plaques: comparison between carotid and femoral beds [J]. Cytokine, 2012, 58(2)∶300-306
[12] Kwan TAT S, Amiable N, Pelletier JP, et al. Modulation of OPG, RANK and RANKL by human chondrocytes and their implication during osteoarthritis [J]. Rheumatology, 2009, 48(12)∶1482-1490
[13] Belibasakis GN, Meier A, Guggenheim B, et al. Oral biofilm challenge regulates the RANKL-OPG system in periodontal ligament and dental pulp cells [J]. Microbial pathogenesis, 2011, 50(1)∶6-11
[14] Regan J, Long F. Notch signaling and bone remodeling [J]. Current osteoporosis reports, 2013, 11(2)∶126-129
[15] 胡芝爱,潘洁,李煜昱,等.DAPT对成牙骨质细胞增殖活性的影响[J].口腔医学研究, 2016, 32(2)∶117-121
[16] Kikuta J, Yamaguchi M, Shimizu M, et al. Notch signaling induces root resorption via RANKL and IL-6 from hPDL cells [J]. J Dent Res, 2015, 94(1)∶140-147