Lipopolysaccharide Enhanced the Expressions of HR in Periodontal Ligment Stem Cells

doi:10.13701/j.cnki.kqyxyj.2018.06.018

Abstract

Abstract: Objective: To investigate whether LPS could regulate the expression of histone demethylases in periodontal ligament stem cells (PDLSCs). Methods: Different doses of LPS were used to stimulate the PDLSCs. The qRT-PCR was used to detect the expression of histone demethylases at the mRNA level. Results: The qRT-PCR results showed that the expression levels of HR were up-regulated at 24 and 48h after LPS treatment in a time dependent manner. And 1, 5, and 10 μg/mL LPS could increase the expression level of HR in a dose dependent manner at 48h. 10 μg/mL LPS inhibited the mineralization of PDLSCs. Conclusion: LPS could induce the expression level of histone demethylase HR in PDLSCs.

Key words: Lipopolysaccharide, Histone demethylase, Periodontal ligament stem cells, Hairless, Osteogenic differentiation

ZHANG Chen, YAO Rui, FAN Zhi-peng. Lipopolysaccharide Enhanced the Expressions of HR in Periodontal Ligment Stem Cells[J]. Journal of Oral Science Research, 2018, 34(6): 648-652.

References

[1] Xu J, Yu B, Hong C,et al. KDM6B epigenetically regulates odontogenic differentiation of dental mesenchymal stem cells [J]. Int J Oral Sci, 2013, 5(4):200-205
[2] Zhang Y, Yan M , Yu QF,et al. Puerarin prevents LPS-induced osteoclast formation and bone loss via inhibition of Akt activation [J]. Biol Pharm Bull, 2016,39(12):2028-2035
[3] Mo IF, Yip KH, Chan WK,et al.Prolonged exposure to bacterial toxins downregulated expression of toll-like receptors in mesenchymal stromal cell-derived osteoprogenitors [J]. BMC Cell Biol, 2008, 9:52
[4] An H, Yu Y, Zhang M,et al.Involvement of ERK, p38 and NF-κB signal transduction in regulation of TLR2, TLR4 and TLR9 gene expression induced by lipopolysaccharide in mouse dendritic cells [J]. Immunology, 2002,106(1):38-45
[5] De Santa F,Narang V, Yap ZH,et al. Jmjd3 contributes to the control of gene expression in LPS-activated macrophages [J]. EMBO J, 2009, 28(21):3341-3352
[6] Das ND, Choi MR, Jung KH,et al. Functional analysis of histone demethylase Jmjd2b on lipopolysaccharide-treated murine neural stem cells (NSCs) [J]. Neurotox Res, 2013,23(2):154-165
[7] 曹钰,杜娟,范志朋.BCOR基因敲除促进骨髓间充质干细胞成骨分化的研究[J].北京口腔医学,2013, 21(2):61-64
[8] 王月君,刘大勇,范志朋,等.维生素C促进根尖牙乳头间充质干细胞组蛋白去甲基化酶KDM2B和KDM5C表达的研究[J].口腔医学研究,2015,31 (5):466-469
[9] 高润涛,范志朋.组蛋白去甲基化酶KDM6A促进骨髓间充质干细胞成骨分化研究[J].北京口腔医学,2014,22 (1):5-8
[10] Zhu W,Liang M.Periodontal ligament stem cells: current status, concerns, and future prospects [J]. Stem Cells Int, 2015, 2015:972313
[11] Djabali K, Aita VM ,Christiano AM. Hairless is translocated to the nucleus via a novel bipartite nuclear localization signal and is associated with the nuclear matrix [J]. J Cell Sci, 2001, 114(2):367-376
[12] Thompson CC. Hairless is a nuclear receptor corepressor essential for skin function [J]. Nucl Recept Signal, 2009, 7:e010
[13] Liu L, Kim H, Casta A,et al. Hairless is a histone H3K9 demethylase [J].FASEB J, 2014, 28(4):1534-1542
[14] Lien WH, Guo X, Polak L,et al. Genome-wide maps of histone modifications unwind in vivo chromatin states of the hair follicle lineage [J]. Cell Stem Cell, 2011, 9(3):219-232
[15] Gilbert L, He X, Farmer P,et al. Inhibition of osteoblast differentiation by tumor necrosis factor-alpha [J]. Endocrinology, 2000,141(11):3956-3964