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    20 June 2018, Volume 34 Issue 6 Previous Issue    Next Issue

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    Application of Socket-shield Technique in the Implantation of Esthetics Zone
    MA Guo-wu, XUE Zang-hui
    2018, 34(6): 581-584.  DOI: 10.13701/j.cnki.kqyxyj.2018.06.001
    Abstract ( 716 )   PDF (940KB) ( 316 )  
    Extraction often occurred in esthetics zone because of various factors, and the labial alveolar bone was rapidly collapsed and absorbed, which caused a serious aesthetic threat to the anterior teeth, but also brought great challenge to the subsequent implant treatment. Therefore, scholars had put forward various schemes to keep the labial alveolar bone. Socket-shield technique could maintain the contour of alveolar ridge for a long time,which might become a new implant treatment program in the esthetics zone.
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    Research Progress about the Role of Transglutaminase 2 in Oral Diseases
    HUANG Hai-yan, SONG Ai-mei, SUN Qin-feng
    2018, 34(6): 585-587.  DOI: 10.13701/j.cnki.kqyxyj.2018.06.002
    Abstract ( 290 )   PDF (773KB) ( 319 )  
    Transglutaminase 2 (TG2), also known as tissue transglutaminase, is a widely distributed,protein cross-linking enzyme with multiple biochemical functions. TG2 has been reported to involve in etiopathogenesis of several pathological conditions and the inhibition of enzyme activity is potential for therapeutic management of these diseases. However, there is no review on the relationship between TG2 and oral diseases. In this paper, the role of TG2 in three oral diseases, including chronic periodontitis, drug induced gingival overgrowth, and oral submucous fibrosis is reviewed, which is to provide a new insight to the therapy of some oral diseases.
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    Research on the Relationship between Oral Pathogens and Alzheimer's Disease
    ZONG Ya-wen,WANG Fei-fei,LI Biao,LIU Bin
    2018, 34(6): 588-590.  DOI: 10.13701/j.cnki.kqyxyj.2018.06.003
    Abstract ( 310 )   PDF (779KB) ( 325 )  
    In recent years, bacterial infection is considered to be one of the mechanisms of Alzheimer's disease. Oral diseases, such as caries disease and periodontal disease, and Alzheimer's disease are involved in a variety of inflammatory mediators which are involved in inflammation. This suggests that oral pathogens may participate in the pathological process of Alzheimer's disease. This paper reviews the relationship between oral pathogens and Alzheimer's disease.
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    A Review on Recent Progress of Platelet Rich Fibrin in Maxillary Sinus Elevation
    WANG Han-chi,ZHAO Jing-hui,LIU Chun-ying,WANG Nan,WANG Yao,ZHOU Yan-min
    2018, 34(6): 591-593.  DOI: 10.13701/j.cnki.kqyxyj.2018.06.004
    Abstract ( 299 )   PDF (772KB) ( 225 )  
    The maxillary posterior region usually lacks sufficient bone mass and cannot support the implant. Through maxillary sinus floor lifting, the bone graft material is filled between the sinus mucosa and the bone plate, which can effectively increase the available bone height and keep the implant stable for a long time. As a platelet concentrate product instead of autogenous bone graft, platelet rich fibrin has the advantages of self-osteogenesis, simple preparation, low price, and so on. In this paper, the progress of platelet rich fibrin in replacing bone graft material for osteogenesis in maxillary sinus augmentation is reviewed.
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    Research Progress of MicroRNA Related to Chemotherapy Resistance in Head and Neck Cancer
    YIN Xi-teng,HAN Wei
    2018, 34(6): 594-596.  DOI: 10.13701/j.cnki.kqyxyj.2018.06.005
    Abstract ( 220 )   PDF (781KB) ( 221 )  
    Head and Neck Cancer (HNC) is one of the most common malignancies in the world. At present, cisplatin based chemotherapy and adjuvant radiotherapy is still the first-line treatment for advanced patients. However, the treatment effect of patients is often limited by the tolerance of cancer cells to chemotherapy drugs, which ultimately leads to treatment failure and death. In recent years, miRNAs has been found to play an important role in tumor progression, metastasis, invasion, and drug resistance. Through literature review, we found that the expression of miRNAs can lead to head and neck cancer resistance through a variety of ways, including the mechanisms of multidrug resistance (MDR) transporters, anti-apoptosis, DNA damage repair, epithelial mesenchymal transition (EMT), cancer stem cells (CSCs), drug target change, and metabolism. This article reviews and explores the use of miRNAs adjuvant chemotherapy strategy.
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    Gene Expression, Purification, and Activity Determination of Alanine Racemase from Streptococcus Mutans
    GUO Xiao, QIU Wei, ZHOU Xue-dong, CHENG Lei, LI Ming-yun
    2018, 34(6): 597-600.  DOI: 10.13701/j.cnki.kqyxyj.2018.06.006
    Abstract ( 276 )   PDF (1030KB) ( 191 )  
    Objective: To purify and measure activity of alanine racemase from Streptococcus mutans (S. mutans). Methods: In the study, expression of recombinant pET-smu.1834c was induced by IPTG, and the protein was purified using Ni-NTA resin with eluotropic buffers. Results: The protein encoded by smu.1834c was successfully purified and it was found that the protein could catalyze the conversion of L-Ala into D-Ala in vitro. Conclusion: D-cycloserine (DCS) was able to inhibit the activity of purified Alanine racemase (Alr) in a dose-dependent manner. This study confirmed that smu.1834c was encoded Alr in S. mutans. DCS can inhibit the activity of Alr.
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    Whole Genome Sequence of Bacillus Subtilis TLO3 and Comparative Genomics Analysis among That Strain, B.subtilis 168 and B.subtilis XF-1
    ZHANG Xiao-feng, HUANG Hui-min, YI Gen-yun, WU Sheng-rong, ZHOU Jian-ye, LI Zhi-qiang, BAO Guang-jie
    2018, 34(6): 601-604.  DOI: 10.13701/j.cnki.kqyxyj.2018.06.007
    Abstract ( 263 )   PDF (2818KB) ( 584 )  
    Objective: To analyze the whole genome and molecular biological function of the B.subtilis TLO3. Methods: The whole genome sequence of B. subtilis TLO3 was performed by a combination of the three-generation and second-generation sequencing technology, and using relevant software for its genome assembly, gene prediction, functional annotation and comparative genomics analysis with B. subtilis 168 and XF-1. Results: The complete genome of B. subtilis TLO3 consists of one circular chromosome and plasmid. The number of genes in cell replication, recombination and repair in strain TLO3 was significantly higher than that of XF-1 and 168, and the antimicrobial gene cluster of TLO3 was similar to XF-1. Conclusion: B.subtilisTLO3, with antimicrobial activity, has a stronger viability and may be better adapted to the complex environment of the oral.
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    Effects of N-Ac-L-Leu-PEI/microRNA-34a Complex on Osteogenisis of Rat Cranial Defects
    HU Tian-qi, SHEN Yu-qin, FEI Hong-bo, LI Yang-yang, GU Zhong-yi, BI Xue-ting, WANG Zhuo-ran, LI Wen-jie, LIN Chong-tao
    2018, 34(6): 605-608.  DOI: 10.13701/j.cnki.kqyxyj.2018.06.008
    Abstract ( 220 )   PDF (1788KB) ( 239 )  
    Objective: To study the effects of N-Ac-L-Leu-PEI/micro RNA(miR)-34a complex on osteogenisis of rat cranial defects. Methods: Thirty-two Wistar male rats were randomly divided into four groups and standard cranial defects were prepared on each rat. Biofilms loaded with PBS, N-Ac-L-Leu-PEI, N-Ac-L-Leu-PEI/miR-34a complex, and N-Ac-L-Leu-PEI/miR-34a NC complex covered the defect. After ten weeks culture, the rats were sacrificed and the development of osteogenisis was detected by CBCT, micro-CT and RT-PCR. Results: Bone formation was evident in the defects of N-Ac-L-Leu-PEI / miR-34a complex group. The BV/TV values and Tb.Th values of N-Ac-L-Leu-PEI/miR-34a complex group increased significantly, suggesting new bone formation. The expression of Runx2, SP7, and Col I mRNA was up-regulated by N-Ac-L-Leu-PEI/miR-34a complex, which were specific factors of osteogenisis. Conclusion: N-Ac-L-Leu-PEI/miR-34a complex can promote osteogenesis in rat cranial defects.
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    Effect of Pure Titanium Suface-loaded Cyclo (RGDfk) on Proliferation and Signal Transduction Pathway of Osteoblasts
    QUAN Yu-jian, LI De-chao, LI Mu-qin, ZHANG Hui-ming, LIU Yang
    2018, 34(6): 609-613.  DOI: 10.13701/j.cnki.kqyxyj.2018.06.009
    Abstract ( 197 )   PDF (969KB) ( 225 )  
    Objective: To study the proliferation and cell viability of osteoblasts in vitro by introducing microencapsulated MAO and dopamine into c(RGDfk) cyclic peptide. Methods: The effects of MC3T3-E1 osteoblasts proliferation and osteogenesis were detected by CCK-8 assay and ALP assay. Western blotting was used to analyze the focal adhesion kinase signal pathway and MAPK signaling pathway related protein content of MC3T3-E1 cells on different coatings. Results: The cell proliferation and activity were significantly affected by microarc oxidation-dopamine-cyclic peptide c (RGDfk) coating on pure titanium surface, which was better than those on pure titanium micro-arc oxidation-dopamine coating. The focal adhesion kinase and MAPK signaling pathway related protein levels and activity of cells in the composite coating were upregulated. Conclusion: The proliferation and signal transduction pathway of MC3T3-E1 osteoblasts were enhanced on the surface of pure titanium after microarc oxidation-dopamine-c (RGDfk) cyclic peptide coating.
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    Effects of PTH-FC on Proliferation and Osteogenic Differentiation of Rat Bone Marrow Mesenchymal Stem Cells
    LIU Yan-yun, XU Ning, LIU Yu-hui, LIU Jia-tao, ZHU Yi-dan, HE Miao-miao, YU Zhan-hai
    2018, 34(6): 614-618.  DOI: 10.13701/j.cnki.kqyxyj.2018.06.010
    Abstract ( 234 )   PDF (3239KB) ( 175 )  
    Objective: To evaluate the effects of PTH-FC on osteogenic differentiation of rat bone marrow mesenchymal stem cells (BMSCs), and to screen out the optimal time of BMSCs induced by PTH-FC. Methods: BMSCs from rats were isolated and cultured in vitro using the whole bone marrow adherence method and identified. BMSCs was induced with PTH-FC (10-9mol/L) osteogenic inducer for 6h (intermittent administration) and 48h (continuous administration). The best proliferation promoting and osteogenic differentiation promoting effect of PTH-FC on BMSCs was evaluated by means of MTT, ALP, and alizarin red staining. Results: ALP and alizarin red staining in the osteogenic culture medium of PTH-FC (10-9mol/L) which acted on BMSCs for 6h were significantly higher than those of other groups (P<0.05), however, the cell proliferation result was not significant different between the experimental groups. Conclusion: The combination of PTH-FC, which is used as a bone induced growth factor, with BMSCs to repair bone tissue defects is feasible.
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    Characteristics of Mandibular Cortical Bone Thickness in Type 1 Diabetic Mice
    SU Ye , SHAO Ling
    2018, 34(6): 619-622.  DOI: 10.13701/j.cnki.kqyxyj.2018.06.011
    Abstract ( 183 )   PDF (1847KB) ( 133 )  
    Objective: To observe the mandible cortical bone thickness changes in type 1 diabetes mellitus mice using Micro-CT. Methods: Twenty 6-week-old mice were randomly divided into control group and diabetes mellitus group. The diabetes mellitus group received intraperitoneal injection of 50 mg/kg streptozotocin for 5 days to establish a type 1 diabetes mellitus model, and the control group received intraperitoneal injection of citrate buffer. Then the mice were sacrificed after 12 weeks. The serum calcium and serum phosphorus levels were detected. Micro-CT technique was used to observe the cortical bone thickness changes of the mandible in type 1 diabetes mellitus mice and control group. Results: Compared with control group, the serum calcium level in diabetes mellitus group was decreased, the serum phosphorus level was increased, and the calcium-phosphorus product level was decreased significantly. Micro-CT showed that the thickness of mandible cortical bone in the interest region of type 1 diabetes mellitus mice was significantly decreased in contrast to that of control group (P<0.01). Conclusion: The streptozotocin -induced type 1 diabetes mellitus mice model suggested that the three-dimensional structure of the cortical bone were changed significantly. Micro-CT showed the thickness change of the cortical bone was more obvious.
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    Expression and Clinical Significance of EGFR, MTDH, and ERCC1 in Special Subtypes of Oral Squamous Carcinoma
    CHEN Wei-hua, PEI Jing, JIA Yun-xiang
    2018, 34(6): 623-626.  DOI: 10.13701/j.cnki.kqyxyj.2018.06.012
    Abstract ( 208 )   PDF (1664KB) ( 229 )  
    Objective: To explore the expression of EGFR, MTDH, and ERCC1 in special subtypes of oral squamous cell carcinoma. Methods: Immunohistochemistry polymer was applied to detect special subtypes of oral squamous cell carcinoma. Results: The expression of EGFR in oral adenosquamous carcinoma was higher than that of basaloid squamous cell carcinoma, papillary squamous cell carcinoma, and spindle cell carcinoma, and the expression in verrucous carcinoma was the weakest(P<0.05). The expressions of MTDH and ERCC1 in oral adenosquamous carcinoma were higher than those of basaloid squamous cell carcinoma, papillary squamous cell carcinoma, and verrucous carcinoma, and the expressions in spindle cell carcinoma was the weakest(P<0.05). The metastasis rate of lymph nodes in oral adenosquamous carcinoma was higher than that of basaloid squamous cell carcinoma, and the rates in papillary squamous cell carcinoma, spindle cell carcinoma, and verrucous carcinoma were weaker(P<0.05). Conclusion: The levels of EGFR, MTDH, and ERCC1 can be used as important biomarkers for evaluation,which may provide the basis for the treatments of special subtypes of oral squamous cell carcinoma.
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    Effects of Long Non-coding RNA HOTAIR on Proliferation, Migration, and Invasion of Tongue Squamous Cell Carcinoma
    DAI Meng-ying, ZHANG Yang, ZHANG Zhao-nan, SU Wei-peng, ZHANG Song-an, LIU Pan, ZHAO Hua-rong
    2018, 34(6): 627-631.  DOI: 10.13701/j.cnki.kqyxyj.2018.06.013
    Abstract ( 197 )   PDF (3167KB) ( 269 )  
    Objective: To investigate the effect of HOTAIR on proliferation, migration, and invasion of tongue squamous cell carcinoma cell line. Methods: The expression of HOTAIR was knocked down by liposome mediated siRNA, and the meaningless sequence was set as the control group. QRT-PCR was used to detect the silencing effect. MTT assay was used to detect the proliferation of each group. The changes of invasion and migration ability of each group was detected by scratch test and Transwell test. Results: HOTAIR was successfully knocked down in the 2 cell lines, and the difference was statistically significant (P<0.001). In the MTT assay, compared with the control group, the absorbance values after 24h, 48h, 72h, and 96h in the experimental group were decreased (P<0.05). In the Transwell test, the membrane cell number of the experimental group was significantly less than that of the control group (P<0.001). The migration distance of the experimental group in the scratch group was less than that of the control group (P<0.001). Conclusion: HOTAIR promotes the proliferation, migration, and invasion of tongue squamous cells carcinoma (Tca-8113, TSCCA), which involved in the development of tongue cancer.
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    Expressions and Clinical Significances of IL-24 in Oral Squamous Cell Carcinoma
    LIU Chong, LI Ji-chen, SAI Yin Wu-liji, PIAO Song-lin, CAO Bo, ZHAO Da-yong
    2018, 34(6): 632-635.  DOI: 10.13701/j.cnki.kqyxyj.2018.06.014
    Abstract ( 244 )   PDF (1482KB) ( 268 )  
    Objective: To study the expressions and clinical significances of interleukin-24 (IL-24) in the oral squamous cell carcinoma (OSCC). Methods: The protein and gene expressions of IL-24 were detected respectively by immunohistochemistry and real-time polymerase chain reaction (real-time PCR) technique method in 75 cases of OSCC patients and 30 cases of controls with normal oral mucosa tissues. The relationship between IL-24 expressions and the clinicopathologic features was analyzed. Results: Immunohistochemical staining showed that the positive expressions of IL-24 in the OSCC (45.33%, 34/75) were significantly lower than those of normal oral mucosa tissue (70.00%, 21/30)(P=0.022). The expressions of IL-24 were closely associated with the differentiation degree (P=0.002), lymph node metastasis (P=0.033) and clinical stage (P=0.035), but they were not correlated with the gender (P=0.917), age (P=0.812), smoking history (P=0.720), drinking history (P=0.847), and the site of lesion (P=0.959). Real-time PCR was used to detect the gene expressions of IL-24 in different pathological differentiation period of OSCC and in normal oral mucosa tissues. The average expressions of IL-24 in highly differentiated OSCC were 0.688, in moderately differentiated OSCC were 0.302, and in poorly differentiated OSCC were 0.153. All of them were lower than the average expressions of IL-24 in normal oral mucosa tissues, which were 1.000 (P<0.001). Conclusion: The expressions of IL-24 in OSCC were lower than that in normal oral mucosa tissues. This gene has certain correlation with clinical information.
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    Assessment of Alveolar Bone Thickness and Height surrounding Maxillary Anterior Teeth in Patients with Unilateral Cleft Lip and Palate: A Cone-beam Computed Tomography Study
    YANG Jia-xin, WANG Jian-guo, ZHANG Xi-zhong, WEI Zhi-qiang
    2018, 34(6): 636-639.  DOI: 10.13701/j.cnki.kqyxyj.2018.06.015
    Abstract ( 266 )   PDF (1254KB) ( 208 )  
    Objective: To study the alveolar bone thickness and height of patients with unilateral cleft lip and palate (UCLP) using cone-beam computed tomography (CBCT). Methods: The CBCT scans of 30 patients with UCLP were assessed. The thickness and height of alveolar bone surrounding the maxillary central incisors and canines were measured using the software Invivo. For each tooth analyzed, the thickness of the buccal and palate bone level at 3mm, 6mm, and 1mm were measured. Meanwhile, we measured the distance between the bone crest (AC) and the cementoenamel junction (CEJ) at the buccal and palate side. A paired t-test was performed to compare bone thickness and AC-CEJ distance at the cleft and non-cleft side of the central incisors and canines. Results: For central incisors, the thickness of the buccal and palate bone level at 3mm and 6mm had statistical difference between the cleft side and non-cleft side (P<0.05). The thickness of alveolar bone at the non-cleft side was larger than that at the cleft side. But for canines, the thickness of the buccal and palate bone had no statistical difference between the cleft side and non-cleft side. For both central incisors and canines, the AC-CEJ distance of the buccal bone had statistical difference between the cleft side and non-cleft side (P<0.05). The AC-CEJ distance at the cleft side was larger than that at the non-cleft side. The AC-CEJ distance of the palate bone had no statistical difference between the cleft side and non-cleft side. Conclusion: For UCLP, the teeth alveolar bone loss and thickness are both in relation to the existence of cleft. It is necessary to accurately assess the alveolar bone by CBCT before orthodontic treatment.
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    A Comparative Analysis of Craniofacial Morphology on Adult Patients with Incomplete Cleft Palate and Normal Adults
    GUO Ling-yan, CHEN Fen, LI Yu, CHEN Lin-lin
    2018, 34(6): 640-643.  DOI: 10.13701/j.cnki.kqyxyj.2018.06.016
    Abstract ( 217 )   PDF (1578KB) ( 210 )  
    Objective: To analysis the craniofacial morphology on adult unoperated incomplete cleft palate(UICP)patients. Methods: Thirty cases of adult UICP patients were selected from the Department of Oral and Maxillofacial Surgery of the Affiliated Stomatological Hospital of Nanchang University (2008-2014) as the research objects. Thirty cases of adults with normal occlusion were selected at the same region as the control group. Each object was taken the lateral and posteroanterior cephalograms. Winceph8.0 cephalometric analysis software was used to compare the UICP patients with the normal controls. Results: By comparing with the normal control group, S-N, N-ANS, ZAL-ZAR, and Gn-Co values were smaller (P<0.05). N-Ba, ANS-Ptm, Co-Go, and SNA were significantly smaller (P<0.01). SN/GoMe were significantly increased in the UICP patients group (P<0.01) . Conclusion: Cleft palate deformities had obvious inhibitory effect on the craniofacial development.
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    Serum Cytokine Response to Periodontal Infection in Overweight Mice
    YU Ting, ZHAO Li, ZHANG Jin-cai, XUAN Dong-ying
    2018, 34(6): 644-647.  DOI: 10.13701/j.cnki.kqyxyj.2018.06.017
    Abstract ( 225 )   PDF (1465KB) ( 171 )  
    Objective: To explore systemic immune response of overweight host to periodontal infection. Methods: Twenty-eight C57 BL/6J mice were divided into high-(HFD) or low-fat diet (LFD) groups to induce overweight or being as controls, respectively. After 8 weeks on diet, both two diet groups were subdivided into periodontitis (P) or periodontal-health controls (C) (n=7), with the P group for periodontal ligation and the C group as controls. The mice were killed after 10 days’ ligation. Linear bone loss fraction was analyzed by micro-CT. Twenty candidate serum cytokines were detected by protein arrays. Differences between the four groups were analyzed by one-way ANOVA. Results: The HFD group had 3.8g or 15.5% higher body weight than that of the LFD group (P<0.01). The P group had significantly higher linear bone loss fraction compared with that of the C group (P<0.01). Except for IL-6, the level of which was significantly higher in the HFDP group relative to the LFDC group (P<0.05), no any other serum cytokines showed significantly different levels between HFD and LFD groups or between P and C groups within the HFD mice. Conclusion: No significant mediating role of systemic inflammation is found in correlation between overweight and periodontitis.
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    Lipopolysaccharide Enhanced the Expressions of HR in Periodontal Ligment Stem Cells
    ZHANG Chen, YAO Rui, FAN Zhi-peng
    2018, 34(6): 648-652.  DOI: 10.13701/j.cnki.kqyxyj.2018.06.018
    Abstract ( 199 )   PDF (2305KB) ( 198 )  
    Objective: To investigate whether LPS could regulate the expression of histone demethylases in periodontal ligament stem cells (PDLSCs). Methods: Different doses of LPS were used to stimulate the PDLSCs. The qRT-PCR was used to detect the expression of histone demethylases at the mRNA level. Results: The qRT-PCR results showed that the expression levels of HR were up-regulated at 24 and 48h after LPS treatment in a time dependent manner. And 1, 5, and 10 μg/mL LPS could increase the expression level of HR in a dose dependent manner at 48h. 10 μg/mL LPS inhibited the mineralization of PDLSCs. Conclusion: LPS could induce the expression level of histone demethylase HR in PDLSCs.
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    Effects of Taccaloniolde on LPS - stimulated Proliferation of Periodontal Ligament Fibroblasts
    ZHOU Qian-wen, ZENG Hao, ZHANG Jia-xin, LIU Gong-qi, WANG Sha, WANG Lie
    2018, 34(6): 653-656.  DOI: 10.13701/j.cnki.kqyxyj.2018.06.019
    Abstract ( 225 )   PDF (1305KB) ( 207 )  
    Objective: To research the effect and mechanism of taccaloniolde on LPS-stimulated proliferation of human periodontal ligament fibroblasts. Methods: Human periodontal ligament fibroblasts were cultured in LPS-containing medium with or without different concentrations of taccaloniolde. At the same time, the blank control group without taccaloniolde was established. After 24, 48, and 72 hours, the cell proliferation was detected by CCK-8 assay and the cell apoptosis was detected by flow cytometry. Results: After 24, 48, and 72 h of drug addition, the relative cell viability in the different doses of taccaloniolde was significantly lower than that stimulated with the control and LPS. Compared with the control group, taccaloniolde could promote apoptosis significantly. Conclusion: Taccaloniolde can significantly inhibited cell proliferation of human periodontal ligament fibroblasts increased by LPS-stimulated, and its possible mechanism is due to promote apoptosis.
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    Influence of Spee's Curve and Crowding on 2D and 3D Measurement of Orthodontic Model
    GONG Cheng, WEN Juan, LI Jia-ling, LI Huang
    2018, 34(6): 657-661.  DOI: 10.13701/j.cnki.kqyxyj.2018.06.020
    Abstract ( 296 )   PDF (1419KB) ( 254 )  
    Objective: To investigate the effect of Spee's curve and crowding on the 2D and 3D measurement of orthodontic model. Methods: Study models of 30 patients with permanent dentition in clinical treatment were collected. They were measured by 2D (plaster models and photocopies) and 3D (didital models). The size of teeth, arch length and width, crowding and the depth of Spee curve were measured with grouping by crowding and the depth of Spee's curve. The results mentioned above were evaluated by one-way ANOVA and further compared by LSD method if difference was significant. Results: The differences of the teeth's sizes influenced by Spee's curve and crowding were statistically significant (P<0.05). Pairwise comparison results showed that the difference between digital and photocopies models was obvious. Maxillary mild crowding group was 1.16±1.34mm (P=0.000), mandibular mild crowding group was (1.14±1.40) mm (P=0.000), and mandibular severe crowding group was (1.35±2.19) mm (P=0.028). With the increasing of the depth of Spee's curve, there were statistically significant difference in the teeth's sizes of plaster models, digital models and photocopies. The difference of digital models and the photocopies in the mild Spee's curve group was (1.70±1.39) mm (P=0.001), and in the severe Spee's curve group was (1.66±1.46) mm (P=0 .002). Conclusion: Three measuring methods had good accuracy. Measuring differences were related to crowding and the depth of the Spee's curve.
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    Study on Cortical Bone Thickness and Safety in Maxillary Posterior Interdental Area in Adult of Skeletal Class Ⅱ Malocclusion by Cone Beam CT
    XUE Mei-rong, DU Bing-li, JING Xuan, WU Xiu-ping
    2018, 34(6): 662-665.  DOI: 10.13701/j.cnki.kqyxyj.2018.06.021
    Abstract ( 267 )   PDF (1209KB) ( 257 )  
    Objective: To provide some references of using micro-implant anchorage in adult of skeletal Class Ⅱ malocclusion by measuring the thickness of buccal cortical bone and the buccolingual bone of different insertion angle type in the maxillary second premolar and first molar area by cone-beam CT (CBCT). Methods: The maxillary posterior area of 30 adult patients (18<year<40) were scanned and reconstructed by CBCT. The cortical bone and the bone thickness were measured at 4, 6, and 8mm from the alveolar crest with 45°, 60°, 90° angle in maxillary posterior interdental area between first molar and second premolar. The data were analyzed statistically. Results: The cortical thickness of buccal bone in maxillary posterior interdental area was thicker at 8mm than 6mm. 4mm was the thinnest with the same insertion angle (P<0.05). The cortical bone thickness was the thickest with 45° insertion angle and the thinnest with 90° insertion angle at the same height level (P<0.05). Conclusion: The study suggested that the conical bone thickness is thicker when the implantation location was more away from the alveolar crest, and the insertion angle with 45° also can provides best cortical bone thickness at same height level.
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    Clinical Study of Maxillary Sinus Floor Elevation through Controlling the Tension of Maxillary Sinus Mucosa
    HE Xiao-ji, LIU Dang-li, SUN Gui-feng, CHENG Tao
    2018, 34(6): 666-670.  DOI: 10.13701/j.cnki.kqyxyj.2018.06.022
    Abstract ( 216 )   PDF (3420KB) ( 280 )  
    Objective: To evaluate the clinical effect of maxillary sinus floor elevation through controlling the tension of maxillary sinus mucosa. Methods: A total of 40 patients received the maxillary sinus floor elevation through controlling the tension of maxillary sinus mucosa from the top of alveolar or lateral window of the maxillary sinus,and the bone substitute material was placed instantly. The implants were placed simultaneously or in other time. The patients were analyzed by cone-beam computed tomography and evaluated by Visual Analogue Scale (VAS) and Verbal Rating Scale (VRS). The ISQ scales were compared between two groups. Results: Penetrating of sinus mucosa was observed in one case, but the case was successfully treated with absorbable collagen membrane (Bio-Gide). There was no complaining during two years following up. Significant statistic difference was found in the VAS and VRS (P<0.05), however, not in the ISQ scale between two groups. Conclusion: Through controlling the tension of maxillary sinus mucosa, the maxillary sinus floor elevation was predictably simple and minimally invasive, and the satisfactory rate of patients was high.
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    Application of Pedicle Palate Connective Tissue Grafts in Immediate Implantation in Maxillary Anterior Region
    SUN Li-bo,HUANG Hai-xia,LAN Yu-yan,HUANG Ting, LI Wang-yang
    2018, 34(6): 671-674.  DOI: 10.13701/j.cnki.kqyxyj.2018.06.023
    Abstract ( 256 )   PDF (1817KB) ( 286 )  
    Objective: To evaluate the clinical effect of single-tooth immediate implantation by pedicle palate connective tissue grafts in the maxillary anterior region. Methods: From June 2014 to May 2016,15 patients with thin gingival type in the maxillary anterior region were treated by single-tooth immediate implantation without flap. Pedicle palate connective tissue grafts were applied for soft tissue augmentation around implant. The survival of connective tissue grafts and the prospect of reconstruction of soft tissue around implants after operation were observed. Results: Pedicle palate connective tissue grafts healed very well and the clinical survival rates were100%. After the completion of repair on the very day,6 months, and 12 months,pink esthetic score (PES) in each period was (11.69±0.84),(11.94±0.70),and (11.97±0.68), respectively. The reconstruction of soft tissue around implants attained the aesthetic effect. Conclusion: For the patients with thin gingival type,single-tooth immediate implantation without flap by pedicle palate connective tissue grafts in the maxillary anterior region can achieve better aesthetic effects.
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    Short-term Clinical Effect Evaluation of Trausim Implants
    WANG Li-ping, GUO Xue-qi, DONG Yu, CHEN Xi-li, CHEN Wei-hong, GE Lin-hu
    2018, 34(6): 675-678.  DOI: 10.13701/j.cnki.kqyxyj.2018.06.024
    Abstract ( 258 )   PDF (2576KB) ( 255 )  
    Objective: To observe the short-term clinical effect of Trausim implants used and provide reference basis for its clinical application. Methods: A total of 24 subjects (46 implants) who received Trausim implants during June, 2016 to December, 2017 at the Implant Department, Stomatology Hospital of Guangzhou Medical University were selected. The success rate, the survival rate, marginal bone loss of dental implants, and dental implant-related complication were followed up. The relevant clinical data were analyzed. Results: All implants were clinically osseointegrated and restored, which were followed up 0.5-1.5 years. There was one case of peri-implantitis, the survival rate was 100% and the success rate was 97.83%. The mesial marginal bone height was (0.15±0.43) mm, and the distal marginal bone height was (0.30±0.47) mm after 0.5-1.5 years follow-up. The mean peri-implant bone resorption was (0.23±0.33) mm. Conclusion: Trausim implants had satisfactory clinical effects in the case of strict control of indications and skilled operation, and its long-term efficacy needs further follow-up.
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    A Case Report of Left Maxillary First Molar with Two Distobuccal Canals
    TENG Na-na, CHENG Yue,GUO Ji-hua
    2018, 34(6): 679-680.  DOI: 10.13701/j.cnki.kqyxyj.2018.06.025
    Abstract ( 307 )   PDF (1703KB) ( 292 )  
    The anatomy of maxillary first molar is complicated, and the variations of numbers of mesiobuccal canals, distobuccal canals, and palatal canals will increase in the difficulty of root canal therapy. This case presents a successful root canal treatment of a left maxillary first molar with five canals including two mesiobuccal canals, two distobuccal canals, and one palatal canal, assisted by opreating microscope and cone beam computed tomography (CBCT).
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    Primary Failure of Eruption with Anterior Teeth Affected: A Case Report
    ZHOU Lian, YAN Lei, XU Hai-tao, LI Min-ze, GUO Yu-dong
    2018, 34(6): 681-682.  DOI: 10.13701/j.cnki.kqyxyj.2018.06.026
    Abstract ( 224 )   PDF (1797KB) ( 192 )  
    This paper reports a case of primary failure of eruption with anterior teeth affected. A 15-year-old girl presented with difficulty chewing with a family history. Clinical examination revealed a left open bite with submerged upper left teeth. Panoramic radiograph revealed the upper left lateral incisor, canine, premolars, and molars were affected in the absence of mechanical obstruction. The diagnosis was primary failure of eruption (PFE).
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