Effect of Pure Titanium Suface-loaded Cyclo (RGDfk) on Proliferation and Signal Transduction Pathway of Osteoblasts

doi:10.13701/j.cnki.kqyxyj.2018.06.009

Abstract

Abstract: Objective: To study the proliferation and cell viability of osteoblasts in vitro by introducing microencapsulated MAO and dopamine into c(RGDfk) cyclic peptide. Methods: The effects of MC3T3-E1 osteoblasts proliferation and osteogenesis were detected by CCK-8 assay and ALP assay. Western blotting was used to analyze the focal adhesion kinase signal pathway and MAPK signaling pathway related protein content of MC3T3-E1 cells on different coatings. Results: The cell proliferation and activity were significantly affected by microarc oxidation-dopamine-cyclic peptide c (RGDfk) coating on pure titanium surface, which was better than those on pure titanium micro-arc oxidation-dopamine coating. The focal adhesion kinase and MAPK signaling pathway related protein levels and activity of cells in the composite coating were upregulated. Conclusion: The proliferation and signal transduction pathway of MC3T3-E1 osteoblasts were enhanced on the surface of pure titanium after microarc oxidation-dopamine-c (RGDfk) cyclic peptide coating.

Key words: Cyclic peptide c(RGDfk), Signal Pathway, Pure Titanium, Microarc Oxidation, Osteoblast

QUAN Yu-jian, LI De-chao, LI Mu-qin, ZHANG Hui-ming, LIU Yang. Effect of Pure Titanium Suface-loaded Cyclo (RGDfk) on Proliferation and Signal Transduction Pathway of Osteoblasts[J]. Journal of Oral Science Research, 2018, 34(6): 609-613.

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