Effect of Inhibition of Wnt/β-catenin Signaling Pathway on Development of Upper Lip

doi:10.13701/j.cnki.kqyxyj.2019.07.018

Abstract

Abstract: Objective: To investigate the effect of inhibiting the Wnt/β-catenin signal on the growth of upper lip. Methods: The maxillary prominence of chicken embryo was implanted the beads impregnated with XAV939 and DMSO and implanted without beads into in the H-H22 stage. The experimental group, control group, and sham group were constructed separately. The incubation was continued until the H-H38 stage. The development of the upper lip of the embryo was observed and recorded by stereomicroscopy and micro-CT. Results: After the Wnt/β-catenin signal was inhibited, the fusion of the maxillary prominence and the frontonasal mass and the lateral nasal prominence of the chicken embryo implanted at the H-H28 stage was delayed, and the upper lip of the chicken embryo implanted-side of the H-H38 stage was delayed in development. The midline was biased toward the implanted side. In addition, the development of the bilateral upper lip of the normal side of the experimental group, the control group, and the sham group was not affected. Conclusion: In the development of the upper lip of the chicken embryo, by inhibition of Wnt/β-catenin signal, the fusion of the maxillary prominence, and the frontonasal mass can be delayed so that the development of the upper lip was delayed, leading to the insufficient development or deformity of maxilla bone.

Key words: Wnt/β-catenin signal pathway , chick embryo, upper lip development

LI Zhicong, ZOU Rui, Hye-Jin Tak, Sang-Hwy Lee, PIAO Zhengguo. Effect of Inhibition of Wnt/β-catenin Signaling Pathway on Development of Upper Lip[J]. Journal of Oral Science Research, 2019, 35(7): 695-700.

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